2006
Analysis of FimX, a phosphodiesterase that governs twitching motility in Pseudomonas aeruginosa
Kazmierczak BI, Lebron MB, Murray TS. Analysis of FimX, a phosphodiesterase that governs twitching motility in Pseudomonas aeruginosa. Molecular Microbiology 2006, 60: 1026-1043. PMID: 16677312, PMCID: PMC3609419, DOI: 10.1111/j.1365-2958.2006.05156.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsCell MovementCyclic GMPEscherichia coli ProteinsFemaleFimbriae, BacterialHeLa CellsHumansMiceMice, Inbred C57BLPhosphoric Diester HydrolasesPhosphorus-Oxygen LyasesPneumonia, BacterialPoint MutationProtein Structure, TertiaryPseudomonas aeruginosaSequence DeletionVirulenceConceptsEAL domainBacterial poleGGDEF-EAL proteinsCyclic dimeric guanosine monophosphateDiguanylate cyclase activityPolar surface structuresType IV piliWild-type strainGGDEF domainDiguanylate cyclasesREC domainLocalization signalPilus assemblyGGDEFNon-polar sitesFimXSurface piliPseudomonas aeruginosaPhosphodiesterase activityBiofilm formationProteinMutantsPiliMotilityDomain
2003
Epithelial Cell Polarity Alters Rho-GTPase Responses to Pseudomonas aeruginosa
Kazmierczak BI, Mostov K, Engel JN. Epithelial Cell Polarity Alters Rho-GTPase Responses to Pseudomonas aeruginosa. Molecular Biology Of The Cell 2003, 15: 411-419. PMID: 14595106, PMCID: PMC329196, DOI: 10.1091/mbc.e03-08-0559.Peer-Reviewed Original ResearchConceptsRho family GTPasesPolarized MDCK monolayersActin polymerizationP. aeruginosa entryRho family GTPase activationMDCK monolayersCdc42-GTP levelsClostridium difficile toxin BOpportunistic human pathogenMadin-Darby canine kidney cellsActivation of RhoAEpithelial cellsDifficile toxin BCanine kidney cellsCell polarityBasolateral infectionMDCK cell monolayersGTPase activationDifferentiation stateP. aeruginosaPseudomonas aeruginosaSurface proteinsBasolateral surfaceHuman pathogensGTPases
2002
Pseudomonas aeruginosa ExoT Acts In Vivo as a GTPase-Activating Protein for RhoA, Rac1, and Cdc42
Kazmierczak B, Engel J. Pseudomonas aeruginosa ExoT Acts In Vivo as a GTPase-Activating Protein for RhoA, Rac1, and Cdc42. Infection And Immunity 2002, 70: 2198-2205. PMID: 11895987, PMCID: PMC127837, DOI: 10.1128/iai.70.4.2198-2205.2002.Peer-Reviewed Original Research
2000
The Arginine Finger Domain of ExoT Contributes to Actin Cytoskeleton Disruption and Inhibition of Internalization ofPseudomonas aeruginosa by Epithelial Cells and Macrophages
Garrity-Ryan L, Kazmierczak B, Kowal R, Comolli J, Hauser A, Engel J. The Arginine Finger Domain of ExoT Contributes to Actin Cytoskeleton Disruption and Inhibition of Internalization ofPseudomonas aeruginosa by Epithelial Cells and Macrophages. Infection And Immunity 2000, 68: 7100-7113. PMID: 11083836, PMCID: PMC97821, DOI: 10.1128/iai.68.12.7100-7113.2000.Peer-Reviewed Original ResearchConceptsEpithelial cellsImportant nosocomial pathogenType III secretion systemHost cellsMacrophage-like cellsAcute pneumoniaMouse modelNosocomial pathogenOfPseudomonas aeruginosaType IIIJ774.1 macrophage-like cellsSecretion systemStrain PA103ExoTPseudomonas aeruginosaCellsCytoskeleton disruptionNegative regulatorPA103Reduced colonizationActin cytoskeleton disruptionPrevious studiesAeruginosaPneumoniaVirulence