2003
Epithelial Cell Polarity Alters Rho-GTPase Responses to Pseudomonas aeruginosa
Kazmierczak BI, Mostov K, Engel JN. Epithelial Cell Polarity Alters Rho-GTPase Responses to Pseudomonas aeruginosa. Molecular Biology Of The Cell 2003, 15: 411-419. PMID: 14595106, PMCID: PMC329196, DOI: 10.1091/mbc.e03-08-0559.Peer-Reviewed Original ResearchConceptsRho family GTPasesPolarized MDCK monolayersActin polymerizationP. aeruginosa entryRho family GTPase activationMDCK monolayersCdc42-GTP levelsClostridium difficile toxin BOpportunistic human pathogenMadin-Darby canine kidney cellsActivation of RhoAEpithelial cellsDifficile toxin BCanine kidney cellsCell polarityBasolateral infectionMDCK cell monolayersGTPase activationDifferentiation stateP. aeruginosaPseudomonas aeruginosaSurface proteinsBasolateral surfaceHuman pathogensGTPases
2001
Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair
Geiser T, Kazmierczak B, Garrity‐Ryan L, Matthay M, Engel J. Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair. Cellular Microbiology 2001, 3: 223-236. PMID: 11298646, DOI: 10.1046/j.1462-5822.2001.00107.x.Peer-Reviewed Original ResearchConceptsGTPase-activating proteinsEpithelial wound repairPseudomonas aeruginosa ExoTRho family GTPasesWound repairPathogen Pseudomonas aeruginosaNosocomial pathogen Pseudomonas aeruginosaP. aeruginosa internalizationEpithelial cellsP. aeruginosaGAP domainGAP activityBacterial proteinsCell roundingCytoskeleton collapseLung epithelial wound repairExoTCell detachmentVivo virulenceProteinEpithelial tissue damageImmune effector cellsLocal host defenseIntact epithelial barrierHost defenseRho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells
Kazmierczak B, Jou T, Mostov K, Engel J. Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells. Cellular Microbiology 2001, 3: 85-98. PMID: 11207623, DOI: 10.1046/j.1462-5822.2001.00091.x.Peer-Reviewed Original ResearchConceptsRho-GTP levelsP. aeruginosa internalizationRho familyEpithelial cellsGTP levelsRho-specific inhibitorRho family GTPasesC3 ADP-ribosyltransferaseRho GTPase activityPathogen Pseudomonas aeruginosaGram-negative pathogen Pseudomonas aeruginosaMadin-Darby canine kidney cellsPseudomonas aeruginosa internalizationCanine kidney cellsNative RhoActive alleleBacterial internalizationGTPase activityP. aeruginosa strain PA103Fusion constructsADP-ribosyltransferaseLatrunculin A.Active Rac1Bacterial uptakeHeLa cells