2019
Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment
Schniederberend M, Williams JF, Shine E, Shen C, Jain R, Emonet T, Kazmierczak BI. Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment. PLOS Pathogens 2019, 15: e1008149. PMID: 31682637, PMCID: PMC6855561, DOI: 10.1371/journal.ppat.1008149.Peer-Reviewed Original ResearchConceptsFlagellar rotationSurface-attached bacteriaGram-negative opportunistic pathogen Pseudomonas aeruginosaOpportunistic pathogen Pseudomonas aeruginosaSwitch complex proteinsSingle polar flagellumBiofilm formationSurface-associated behaviorsSurface-associated structuresType IV piliPathogen Pseudomonas aeruginosaGenetic screenPolar flagellumTranscriptional programsBiofilm initiationComplex proteinsMutant bacteriaFlagellar attachmentSecond messengerP. aeruginosaFlhFBacteriaFlagellaPathwayAltered behavior
2017
Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations
Kazmierczak BI. Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations. Methods In Molecular Biology 2017, 1657: 23-29. PMID: 28889283, DOI: 10.1007/978-1-4939-7240-1_3.Peer-Reviewed Original ResearchInteraction of the cyclic-di-GMP binding protein FimX and the Type 4 pilus assembly ATPase promotes pilus assembly
Jain R, Sliusarenko O, Kazmierczak BI. Interaction of the cyclic-di-GMP binding protein FimX and the Type 4 pilus assembly ATPase promotes pilus assembly. PLOS Pathogens 2017, 13: e1006594. PMID: 28854278, PMCID: PMC5595344, DOI: 10.1371/journal.ppat.1006594.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCarrier ProteinsChromatography, GelCyclic GMPFimbriae, BacterialImage Processing, Computer-AssistedIntracellular Signaling Peptides and ProteinsMicroscopy, Electron, TransmissionOxidoreductasesPolymerase Chain ReactionPseudomonas aeruginosaSurface Plasmon ResonanceVirulenceVirulence FactorsConceptsT4P assemblyAssembly ATPaseTwitching motilityPoint mutant alleleBacterial cell envelopeType IVa piliGMP receptorPilus assemblyBacterial surface structuresCell envelopeFimXLocalization patternsMutant allelesSecond messengerPilBBiofilm formationATPase activityDirectional movementIntracellular levelsPilTT4PATPaseBacteriaMotilityAssembly