2012
Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations
Jain R, Behrens AJ, Kaever V, Kazmierczak BI. Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations. Journal Of Bacteriology 2012, 194: 4285-4294. PMID: 22685276, PMCID: PMC3416225, DOI: 10.1128/jb.00803-12.Peer-Reviewed Original ResearchConceptsType IV piliPilus assemblyDiguanylate cyclasesSurface organellesGGDEF/EAL domain proteinsType IV pilus assemblyPolar type IV piliEAL domain proteinsAssembly of piliGMP concentrationSuppressor screenCaulobacter crescentusDiguanylate cyclaseDomain proteinsSuppressor mutationsAdditional regulatorsSuppressor strainsRobust biofilmsDifferent environmental conditionsAssembly defectsNegative regulatorMicrocolony formationSurface piliFimXP. aeruginosa
2000
The Arginine Finger Domain of ExoT Contributes to Actin Cytoskeleton Disruption and Inhibition of Internalization ofPseudomonas aeruginosa by Epithelial Cells and Macrophages
Garrity-Ryan L, Kazmierczak B, Kowal R, Comolli J, Hauser A, Engel J. The Arginine Finger Domain of ExoT Contributes to Actin Cytoskeleton Disruption and Inhibition of Internalization ofPseudomonas aeruginosa by Epithelial Cells and Macrophages. Infection And Immunity 2000, 68: 7100-7113. PMID: 11083836, PMCID: PMC97821, DOI: 10.1128/iai.68.12.7100-7113.2000.Peer-Reviewed Original ResearchConceptsEpithelial cellsImportant nosocomial pathogenType III secretion systemHost cellsMacrophage-like cellsAcute pneumoniaMouse modelNosocomial pathogenOfPseudomonas aeruginosaType IIIJ774.1 macrophage-like cellsSecretion systemStrain PA103ExoTPseudomonas aeruginosaCellsCytoskeleton disruptionNegative regulatorPA103Reduced colonizationActin cytoskeleton disruptionPrevious studiesAeruginosaPneumoniaVirulence