2017
Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations
Kazmierczak BI. Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations. Methods In Molecular Biology 2017, 1657: 23-29. PMID: 28889283, DOI: 10.1007/978-1-4939-7240-1_3.Peer-Reviewed Original Research
2012
Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations
Jain R, Behrens AJ, Kaever V, Kazmierczak BI. Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations. Journal Of Bacteriology 2012, 194: 4285-4294. PMID: 22685276, PMCID: PMC3416225, DOI: 10.1128/jb.00803-12.Peer-Reviewed Original ResearchConceptsType IV piliPilus assemblyDiguanylate cyclasesSurface organellesGGDEF/EAL domain proteinsType IV pilus assemblyPolar type IV piliEAL domain proteinsAssembly of piliGMP concentrationSuppressor screenCaulobacter crescentusDiguanylate cyclaseDomain proteinsSuppressor mutationsAdditional regulatorsSuppressor strainsRobust biofilmsDifferent environmental conditionsAssembly defectsNegative regulatorMicrocolony formationSurface piliFimXP. aeruginosa