2019
Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in mice
Veit M, Ahrens B, Seidel J, Sommer A, Bhakdi S, Reiss K. Mutagenesis of the ADAM17-phosphatidylserine–binding motif leads to embryonic lethality in mice. Life Science Alliance 2019, 2: e201900430. PMID: 31455669, PMCID: PMC6712283, DOI: 10.26508/lsa.201900430.Peer-Reviewed Original ResearchConceptsEmbryonic lethalityVital cellular functionsAnionic phospholipid headgroupsTransmembrane substratesCellular functionsProtease functionBinding motifAmino acidsCell surfaceMutant proteasePrimary hepatocytesMotifMutagenesisPhosphatidylserinePhospholipid headgroupsProminent memberCentral roleEssential triggerLethalitySurface exposureOncogenesisProteaseADAM17DisintegrinMice
2012
Melittin Modulates Keratinocyte Function through P2 Receptor-dependent ADAM Activation*
Sommer A, Fries A, Cornelsen I, Speck N, Koch-Nolte F, Gimpl G, Andrä J, Bhakdi S, Reiss K. Melittin Modulates Keratinocyte Function through P2 Receptor-dependent ADAM Activation*. Journal Of Biological Chemistry 2012, 287: 23678-23689. PMID: 22613720, PMCID: PMC3390642, DOI: 10.1074/jbc.m112.362756.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAM10 ProteinADAM17 ProteinAdenosine TriphosphateAmyloid Precursor Protein SecretasesAnimalsBlotting, WesternCadherinsCell LineCell SurvivalCells, CulturedDose-Response Relationship, DrugEmbryo, MammalianErbB ReceptorsExtracellular Signal-Regulated MAP KinasesFibroblastsHEK293 CellsHumansKeratinocytesMelittenMembrane ProteinsMiceMice, KnockoutModels, BiologicalPhosphorylationReceptors, Purinergic P2X7Reverse Transcriptase Polymerase Chain ReactionConceptsAnti-cancer agentsP2 receptorsADAM activationP2 receptor antagonistsRelease of TGFMultiple cellular functionsPurinergic P2 receptorsAdhesion molecule E-cadherinE-cadherinReceptor antagonistP2X7 receptorHuman HaCaT keratinocytesCellular functionsPresence of ATPasesDownstream eventsATP releaseEGFR phosphorylationHuman neutrophilsEGF receptorEndothelial cellsERK1/2 phosphorylationKeratinocyte proliferationReceptorsBee venomCell migration