Metabolism of cancer cells commonly responds to irradiation by a transient early mitochondrial shutdown
Krysztofiak A, Szymonowicz K, Hlouschek J, Xiang K, Waterkamp C, Larafa S, Goetting I, Vega-Rubin-de-Celis S, Theiss C, Matschke V, Hoffmann D, Jendrossek V, Matschke J. Metabolism of cancer cells commonly responds to irradiation by a transient early mitochondrial shutdown. IScience 2021, 24: 103366. PMID: 34825138, PMCID: PMC8603201, DOI: 10.1016/j.isci.2021.103366.Peer-Reviewed Original ResearchCommon metabolic responseCompensatory glycolysisDSB repair kineticsMitochondrial respiratory chainRepair kineticsRadiation-induced DNAFuture mechanistic studiesDNA repairDNA repair kineticsRespiratory chainCancer metabolismMitochondrial functionStress conditionsMetabolic vulnerabilitiesCancer cell linesMetabolic targetsCancer cellsCell linesMitochondrial recoveryGlycolysisNew hypothesisMetabolic responseMetabolismMechanistic studiesDiscoveryHigh-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation
Oeck S, Malewicz NM, Krysztofiak A, Turchick A, Jendrossek V, Glazer PM. High-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation. Scientific Reports 2019, 9: 3148. PMID: 30816253, PMCID: PMC6395640, DOI: 10.1038/s41598-019-39760-8.Peer-Reviewed Original ResearchConceptsDNA damage sitesDNA repair proteinsProtein of interestHistone dynamicsHistone phosphorylationProtein fociHigh-throughput analysisProtein modificationRepair proteinsDNA damageDamage sitesSingle nucleusMultiple fluorescence channelsHigh-throughput evaluationRepair kineticsProtein migrationProteinAnalysis of accumulationFluorescence channelsPhosphorylationDNACellsAccumulationLocalizationMigration