2024
Vascular Endothelial Cells Derived from Transgene-Free Pig Induced Pluripotent Stem Cells for Vascular Tissue Engineering
Batty L, Park J, Qin L, Riaz M, Lin Y, Xu Z, Gao X, Li X, Lopez C, Zhang W, Hoareau M, Fallon M, Huang Y, Luo H, Luo J, Ménoret S, Li P, Jiang Z, Smith P, Sachs D, Tellides G, Anegon I, Pober J, Liu P, Qyang Y. Vascular Endothelial Cells Derived from Transgene-Free Pig Induced Pluripotent Stem Cells for Vascular Tissue Engineering. Acta Biomaterialia 2024 PMID: 39681154, DOI: 10.1016/j.actbio.2024.12.033.Peer-Reviewed Original ResearchInduced pluripotent stem cellsVascular tissue engineeringPig induced pluripotent stem cellsPluripotent stem cellsEndothelial cellsLarge animal modelStem cellsAnimal modelsTissue engineeringInferior vena cava graftHuman induced pluripotent stem cellsEffective differentiation protocolsPreclinical large animal modelExpression of endothelial markersCell-based therapiesExtensive preclinical testingPig endothelial cellsFunctional endothelial cellsIn vivo functional studiesTreatment of cardiovascular diseasesVascular endothelial cellsTissue engineering therapiesTransplant therapeuticsEfficacy of tissueImmunodeficient rats
2021
Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Li ML, Luo J, Ellis MW, Riaz M, Ajaj Y, Qyang Y. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells. Methods In Molecular Biology 2021, 2375: 21-34. PMID: 34591296, DOI: 10.1007/978-1-0716-1708-3_3.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsVascular smooth muscle cellsPluripotent stem cellsLateral plate mesodermEarly embryonic developmentStem cellsSmooth muscle cellsHuman pluripotent stem cellsInduced pluripotent stem cellsExtracellular matrix proteinsMuscle cellsMesoderm lineagePlate mesodermEmbryonic developmentVascular cell sourceEmbryoid bodiesEB formationMatrix proteinsCellular interactionsDisease modelingPhysiological characteristicsVascular tissueTissue-engineered vascular graftsCell-based therapiesCell replacement
2011
High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells
Lee MY, Bozkulak E, Schliffke S, Amos PJ, Ren Y, Ge X, Ehrlich BE, Qyang Y. High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells. Biochemical And Biophysical Research Communications 2011, 416: 51-57. PMID: 22079290, PMCID: PMC3237870, DOI: 10.1016/j.bbrc.2011.10.140.Peer-Reviewed Original ResearchConceptsMurine embryonic stem cellsEmbryonic stem cellsBone morphogenetic proteinEB culturesCardiac differentiationCardiomyocyte productionEmbryoid body culturesStem cellsCardiac-specific genesEarly cardiac developmentCardiogenic growth factorsNK2 transcription factorCardiac regenerative medicineMESC linesTranscription factorsCardiac developmentMorphogenetic proteinsEmbryoid bodiesDifferentiation systemDifferentiation periodFunctional cardiomyocytesHigh-density culturesMyosin light chain 2vRegenerative medicineCell-based therapies