2016
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast
Laplante C, Huang F, Tebbs IR, Bewersdorf J, Pollard TD. Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5876-e5885. PMID: 27647921, PMCID: PMC5056082, DOI: 10.1073/pnas.1608252113.Peer-Reviewed Original ResearchConceptsFluorescence photoactivation localization microscopyContractile ringLive fission yeast cellsLive fission yeastFission yeast cellsCytokinetic contractile ringSuper-resolution fluorescence microscopyCytokinesis nodesPhotoactivation localization microscopyFission yeastFormin Cdc12pActin networkIQ motifPlasma membraneCytokinesisConstituent proteinsMyosin-II tailYeast cellsActin ringsActin strandsAssembly precursorsDiscrete structural unitsFluorescence microscopyMolecular organizationProteinAvoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins
Courtemanche N, Pollard TD, Chen Q. Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins. Nature Cell Biology 2016, 18: 676-683. PMID: 27159499, PMCID: PMC5509211, DOI: 10.1038/ncb3351.Peer-Reviewed Original Research
2015
Three Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring
Laplante C, Berro J, Karatekin E, Hernandez-Leyva A, Lee R, Pollard TD. Three Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring. Current Biology 2015, 25: 1955-1965. PMID: 26144970, PMCID: PMC4526439, DOI: 10.1016/j.cub.2015.06.018.Peer-Reviewed Original ResearchConceptsContractile ringActin filamentsHeavy chain geneMyosin IIContractile ring assemblyFission yeast cellsMyosin heavy chain geneCytokinetic contractile ringConventional myosin IICytokinetic nodesMyo2Myo51Myp2Ring assemblyYeast cellsMyosin functionCytokinesisDeletion mutationsComplete assemblyMyosin VConstriction rateGenesMutationsCellsFilaments
2014
Mechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast
Stachowiak MR, Laplante C, Chin HF, Guirao B, Karatekin E, Pollard TD, O’Shaughnessy B. Mechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast. Developmental Cell 2014, 29: 547-561. PMID: 24914559, PMCID: PMC4137230, DOI: 10.1016/j.devcel.2014.04.021.Peer-Reviewed Original ResearchCytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase
Akamatsu M, Berro J, Pu KM, Tebbs IR, Pollard TD. Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase. Journal Of Cell Biology 2014, 204: 977-988. PMID: 24637325, PMCID: PMC3998791, DOI: 10.1083/jcb.201307174.Peer-Reviewed Original Research
2007
Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast
Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast. Science 2007, 319: 97-100. PMID: 18079366, DOI: 10.1126/science.1151086.Peer-Reviewed Original ResearchConceptsContractile ringActin filamentsLive fission yeast cellsIndividual daughter cellsFission yeast cellsDynamic actin filamentsFission yeastMotor protein myosinCell equatorDaughter cellsYeast cellsAssembly mechanismProtein myosinFluorescence microscopyCytokinesisMyosinFilamentsTransient connectionsCellsYeastFungiMechanismMeshwork