2016
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast
Laplante C, Huang F, Tebbs IR, Bewersdorf J, Pollard TD. Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5876-e5885. PMID: 27647921, PMCID: PMC5056082, DOI: 10.1073/pnas.1608252113.Peer-Reviewed Original ResearchConceptsFluorescence photoactivation localization microscopyContractile ringLive fission yeast cellsLive fission yeastFission yeast cellsCytokinetic contractile ringSuper-resolution fluorescence microscopyCytokinesis nodesPhotoactivation localization microscopyFission yeastFormin Cdc12pActin networkIQ motifPlasma membraneCytokinesisConstituent proteinsMyosin-II tailYeast cellsActin ringsActin strandsAssembly precursorsDiscrete structural unitsFluorescence microscopyMolecular organizationProtein
2011
Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast
Arasada R, Pollard TD. Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast. Current Biology 2011, 21: 1450-1459. PMID: 21885283, PMCID: PMC3350781, DOI: 10.1016/j.cub.2011.07.046.Peer-Reviewed Original ResearchConceptsF-BAR proteinsNucleation-promoting factorsActin patchesFission yeastArp2/3 complexActin polymerizationActin filamentsGenetic interaction experimentsSites of endocytosisPeripheral membrane proteinsActin binding proteinsClathrin-mediated endocytosisMembrane scissionYeast showSH3 domainCdc15pMembrane proteinsMembrane tubulesPlasma membraneGenetic analysisBinding proteinDistinct rolesEndocytosisProteinClathrin