2021
Distinct functions of POT1 proteins contribute to the regulation of telomerase recruitment to telomeres
Gu P, Jia S, Takasugi T, Tesmer VM, Nandakumar J, Chen Y, Chang S. Distinct functions of POT1 proteins contribute to the regulation of telomerase recruitment to telomeres. Nature Communications 2021, 12: 5514. PMID: 34535663, PMCID: PMC8448735, DOI: 10.1038/s41467-021-25799-7.Peer-Reviewed Original ResearchConceptsDNA damage responseTelomerase recruitmentPOT1 proteinsDamage responseATR-dependent DNA damage responseNon-homologous end-joining DNA repair pathwayRecruitment of telomeraseC-strand fillAmino acidsDNA repair pathwaysUnique amino acidsTEN1 (CST) complexTelomere extensionCTC1-STN1Stable heterodimerRepair pathwaysC-terminusDistinct functionsPOT1bPOT1aTelomeresC-strandG-strandTPP1Protein
2017
Mechanisms of precise genome editing using oligonucleotide donors
Kan Y, Ruis B, Takasugi T, Hendrickson E. Mechanisms of precise genome editing using oligonucleotide donors. Genome Research 2017, 27: 1099-1111. PMID: 28356322, PMCID: PMC5495063, DOI: 10.1101/gr.214775.116.Peer-Reviewed Original ResearchConceptsPrecise genome editingSynthesis-dependent strand annealingGenome editingHuman cellsStrand annealingConversion tractsDouble-stranded DNA donorsGenomic lesionsHomology-directed repair (HDR) pathwayCRISPR/Cas9Short conversion tractsFunctional genomicsGene conversionRepair pathwaysShort conversionIncorporation pathwayOligonucleotide donorsHomology donorsDNA donorPathwayCas9EditingUnprecedented easeCellsMeganucleases