Mechanisms of precise genome editing using oligonucleotide donors
Kan Y, Ruis B, Takasugi T, Hendrickson E. Mechanisms of precise genome editing using oligonucleotide donors. Genome Research 2017, 27: 1099-1111. PMID: 28356322, PMCID: PMC5495063, DOI: 10.1101/gr.214775.116.Peer-Reviewed Original ResearchConceptsPrecise genome editingSynthesis-dependent strand annealingGenome editingHuman cellsStrand annealingConversion tractsDouble-stranded DNA donorsGenomic lesionsHomology-directed repair (HDR) pathwayCRISPR/Cas9Short conversion tractsFunctional genomicsGene conversionRepair pathwaysShort conversionIncorporation pathwayOligonucleotide donorsHomology donorsDNA donorPathwayCas9EditingUnprecedented easeCellsMeganucleases