2017
Muscarinic acetylcholine receptors participate in small intestinal mucosal homeostasis
Greig CJ, Cowles RA. Muscarinic acetylcholine receptors participate in small intestinal mucosal homeostasis. Journal Of Pediatric Surgery 2017, 52: 1031-1034. PMID: 28359586, DOI: 10.1016/j.jpedsurg.2017.03.037.Peer-Reviewed Original ResearchConceptsCrypt proliferation indexIntestinal mucosal homeostasisMuscarinic acetylcholine receptorsMucosal homeostasisIleal mucosaAcetylcholine receptorsDistal ileal segmentCholinergic nervous systemWild-type miceCrypt cell proliferationCrypt base stem cellsFunctional mucosaMAChR subtypesMuscarinic signalingIleal segmentCrypt depthProliferation indexNervous systemVillus heightMucosal morphometryMucosaHistologic sectionsMiceMouse linesEpithelial cell types
2016
Distribution of muscarinic acetylcholine receptor subtypes in the murine small intestine
Muise ED, Gandotra N, Tackett JJ, Bamdad MC, Cowles RA. Distribution of muscarinic acetylcholine receptor subtypes in the murine small intestine. Life Sciences 2016, 169: 6-10. PMID: 27866962, DOI: 10.1016/j.lfs.2016.10.030.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescent Antibody TechniqueIntestinal MucosaIntestine, SmallMaleMice, Inbred C57BLMicroscopy, FluorescenceReceptors, MuscarinicStem CellsConceptsSmall intestineReceptor subtypesMuscarinic acetylcholine receptor subtypesShort bowel syndromeEffects of acetylcholineStem cell compartmentMuscarinic receptor subtypesAcetylcholine receptor subtypesSmall intestinal mucosaPotential therapeutic targetMurine small intestineCell compartmentSpecific mAChRBowel syndromeCholinergic pathwaysMyenteric plexusAnatomic distributionMuscarinic receptorsEntire alimentary tractIntestinal diseaseIntestinal mucosaEnterocyte turnoverIntestinal growthTherapeutic targetCrypt bases
2001
Cholinergic stimulation of rat acinar cells increases c-fos and c-jun expression via a mitogen-activated protein kinase-dependent pathway
Turner DJ, Cowles RA, Segura BJ, Mulholland MW. Cholinergic stimulation of rat acinar cells increases c-fos and c-jun expression via a mitogen-activated protein kinase-dependent pathway. Journal Of Gastrointestinal Surgery 2001, 5: 661-672. PMID: 12086906, DOI: 10.1016/s1091-255x(01)80110-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, WesternCalciumCalcium SignalingCarbacholCells, CulturedCholinergic AgonistsDiaminesDose-Response Relationship, DrugEnzyme ActivationGene ExpressionMolecular Sequence DataPancreasPiperidinesPirenzepinePolymerase Chain ReactionProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junRatsReceptors, MuscarinicSensitivity and SpecificityConceptsC-jun early response geneMitogen-activated protein kinase activityProtein kinase-dependent pathwayMitogen-activated protein kinase-dependent pathwayProtein kinase activityAR42J acinar cellsEarly response genesKinase-dependent pathwayDose-dependent increaseC-fosAcetylcholine analogue carbacholC-jun expressionAcinar cellsKinase activityGene expressionM2 inhibitionRat acinar cellsMuscarinic receptor subtypes M1Enzyme activationSpecific inhibitorM3 receptor subtypePolymerase chain reaction analysisChain reaction analysisPancreatic exocrine functionIntracellular calcium release