Structural insights into peptide bond formation
Hansen JL, Schmeing TM, Moore PB, Steitz TA. Structural insights into peptide bond formation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 11670-11675. PMID: 12185246, PMCID: PMC129327, DOI: 10.1073/pnas.172404099.Peer-Reviewed Original ResearchConceptsP-site substrateBond formationHydrogen bondsSecond hydrogen bondPeptide bond formationCarbonyl carbonNucleophilic attackProduct analoguesAmino groupsAlpha-amino groupIntermediate analogueSubstrate complexStructural insightsP siteBondsSite substrateOHHaloarcula marismortui 50S ribosomal subunitAnaloguesSubstrateRibosomal subunitPresence of sparsomycinFormationP-loopComplexesA pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits
Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA. A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits. Nature Structural & Molecular Biology 2002, 9: 225-230. PMID: 11828326, DOI: 10.1038/nsb758.Peer-Reviewed Original ResearchConceptsBond formationFormation of productsPeptide bond formationA-siteSynthesisSubstrate resultsCrystals