1984
Method for determining whether a gene of Escherichia coli is essential: application to the polA gene
Joyce C, Grindley N. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. Journal Of Bacteriology 1984, 158: 636-643. PMID: 6233260, PMCID: PMC215477, DOI: 10.1128/jb.158.2.636-643.1984.Peer-Reviewed Original ResearchConceptsPolA geneTarget genesRich mediumEscherichia coliPhage lambda vectorTarget lociBacterial chromosomeHomologous recombinationPhysical mapProphage excisionDNA segmentsMinimal mediumAntibiotic resistance genesPolymerase 3Recombinational eventsLambda vectorGenesResistance genesChromosomal deletionsDeletionFunctional fragmentsExonucleaseMarked deletionPhagesPresence of plasmids
1976
Effects of different alleles of the E. coli K12 polA gene on the replication of non-transferring plasmids
Grindley N, Kelley W. Effects of different alleles of the E. coli K12 polA gene on the replication of non-transferring plasmids. Molecular Genetics And Genomics 1976, 143: 311-318. PMID: 765763, DOI: 10.1007/bf00269409.Peer-Reviewed Original ResearchMeSH KeywordsAllelesChloramphenicolDNA NucleotidyltransferasesDNA ReplicationDrug Resistance, MicrobialEscherichia coliExtrachromosomal InheritancePhenotypePlasmidsTransduction, Genetic