2012
Remote control of DNA-acting enzymes by varying the Brownian dynamics of a distant DNA end
Bai H, Kath J, Zörgiebel F, Sun M, Ghosh P, Hatfull G, Grindley N, Marko J. Remote control of DNA-acting enzymes by varying the Brownian dynamics of a distant DNA end. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 16546-16551. PMID: 23011800, PMCID: PMC3478594, DOI: 10.1073/pnas.1203118109.Peer-Reviewed Original Research
1990
Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.
Polesky A, Steitz T, Grindley N, Joyce C. Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli. Journal Of Biological Chemistry 1990, 265: 14579-14591. PMID: 2201688, DOI: 10.1016/s0021-9258(18)77342-0.Peer-Reviewed Original ResearchConceptsCluster of residuesIdentification of residuesSite-directed mutagenesisActive site residuesAmino acid residuesFuture mutational studiesImportant active site residuesDNA-binding propertiesActive site regionDNA polymerase IGenetic screenPosition 849Polymerase active siteMutant proteinsDNA substratesMutational studiesPolymerase IBiochemical experimentsSite residuesAcid residuesSite regionEscherichia coliPolymerase activityMutationsPolymerase reaction