2003
Sex steroid hormone metabolism takes place in human ocular cells
Coca-Prados M, Ghosh S, Wang Y, Escribano J, Herrala A, Vihko P. Sex steroid hormone metabolism takes place in human ocular cells. The Journal Of Steroid Biochemistry And Molecular Biology 2003, 86: 207-216. PMID: 14568574, DOI: 10.1016/j.jsbmb.2003.08.001.Peer-Reviewed Original ResearchConceptsSex steroid hormone metabolismCiliary epitheliumHuman ocular cellsSex steroid hormonesHuman ciliary epithelial cellsSteroid hormone metabolismAndrogen agonistsCiliary epithelial cellsEstrogenic milieuOcular diseasesOcular cellsSteroid hormonesMRNA expressionImportant mediatorOxidative enzymatic activityHuman ciliary epitheliumGene expressionEpithelial cellsHormone metabolismHormone-responsive genesSecretory epitheliumEpitheliumH time courseCell linesTime courseA3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway
Shi C, Szczesniak A, Mao L, Jollimore C, Coca‐Prados M, Hung O, Kelly ME. A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway. British Journal Of Pharmacology 2003, 139: 475-486. PMID: 12788807, PMCID: PMC1573867, DOI: 10.1038/sj.bjp.0705266.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TransformedChloride ChannelsCiliaDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsEpithelial CellsGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsHumansMAP Kinase Signaling SystemProtein Kinase CReceptor, Adenosine A3Receptor, Cannabinoid, CB1ConceptsCB1 receptorsCl- currentSR 141716Receptor agonistNPCE cellsA3 adenosineWhole-cell patch-clamp recordingsCB1 receptor inverse agonistBasal Cl(-) currentCB1 receptor agonistA3 receptor agonistPatch-clamp recordingsHuman CB1 receptorReceptor-transfected cellsReceptor inverse agonistHuman nonpigmented ciliary epithelial cellsSignaling pathwaysAdenosine receptor antagonistNonpigmented ciliary epithelial cellsCiliary epithelial cell linePI3K inhibitorsMitogen-activated protein kinase kinase inhibitorBeta-adrenergic receptor kinaseProtein kinase kinase inhibitorEpithelial cell line
2000
Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2000, 279: c440-c451. PMID: 10913011, DOI: 10.1152/ajpcell.2000.279.2.c440.Peer-Reviewed Original Research
1999
Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells
Ortego J, Coca-Prados M. Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells. Biochemical And Biophysical Research Communications 1999, 258: 21-28. PMID: 10222228, DOI: 10.1006/bbrc.1999.0573.Peer-Reviewed Original ResearchConceptsNatriuretic peptide systemNPR-B receptorNPR-C receptorCiliary epithelial cellsReceptor mRNACiliary epitheliumEpithelial cellsNPE cellsCGMP responseAutocrine/paracrine modulatorPeptide systemHuman ocular ciliary epitheliumNonpigmented ciliary epithelial cellsOcular ciliary epitheliumCultured NPE cellsLong-term downregulationC-ANP4Paracrine modulatorEye pressureBNPActivation of PKCBasal levelsInhibitory effectRT-PCRReceptors
1995
PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln
Coca-Prados M, Anguita J, Chalfant M, Civan M. PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln. American Journal Of Physiology 1995, 268: c572-c579. PMID: 7534980, DOI: 10.1152/ajpcell.1995.268.3.c572.Peer-Reviewed Original ResearchMeSH KeywordsAlkaloidsAnimalsBase SequenceBlotting, NorthernCells, CulturedChild, PreschoolChloride ChannelsCiliaCystic Fibrosis Transmembrane Conductance RegulatorElectric ConductivityEpitheliumHumansIon ChannelsKineticsMembrane ProteinsMolecular Sequence DataPolymerase Chain ReactionProtein Kinase CRatsRNA, MessengerStaurosporine