2006
Somatostatin modulates PI3K-Akt, eNOS and NHE activity in the ciliary epithelium
Ghosh S, Choritz L, Geibel J, Coca-Prados M. Somatostatin modulates PI3K-Akt, eNOS and NHE activity in the ciliary epithelium. Molecular And Cellular Endocrinology 2006, 253: 63-75. PMID: 16764985, DOI: 10.1016/j.mce.2006.05.002.Peer-Reviewed Original ResearchConceptsP-Akt Ser473Ciliary epitheliumIntracellular cyclic GMP productionInvolvement of somatostatinDose-dependent attenuationReceptor mRNA expressionCyclic GMP productionSST-like immunoreactivityLack of responseOcular ciliary epitheliumAqueous humorSomatostatinNHE activityExchanger activityMRNA expressionCiliary processesNeuroendocrine cellsPC2 antibodyPI3K-AktMultiple intracellularGMP productionConvertases PC1Increases phosphorylationTissue extractsEpithelium
1996
Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells
CIVAN M, COCA-PRADOS M, PETERSON-YANTORNO K. Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells. Experimental Eye Research 1996, 62: 627-640. PMID: 8983945, DOI: 10.1006/exer.1996.0074.Peer-Reviewed Original ResearchConceptsRegulatory volume increaseCiliary epithelial cellsEpithelial cellsNon-pigmented ciliary epithelial cellsPKC inhibitor staurosporineHuman non-pigmented ciliary epithelial cellsElectronic cell sizingRegulatory volume responsesAcid metabolismSame cellsTransport inhibitorsStaurosporineHuman NonSymport
1995
Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium.
Coca-Prados M, Fernández-Cabezudo M, Sánchez-Torres J, Crabb J, Ghosh S. Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1995, 36: 2717-28. PMID: 7499094.Peer-Reviewed Original ResearchConceptsBeta 2 isoformNonpigmented ciliary epithelial cellsOcular tissuesCiliary epitheliumBeta 1NPE cell linesNonpigmented ciliary epitheliumNPE cellsHuman ocular tissuesSubunit isoformsBlot analysisATPase subunit isoformsSpecific anti-peptide antibodiesRestricted cellular distributionPolymerase chain reactionCiliary epithelial cellsBeta-subunit isoformsAlpha-subunit isoformsAnti-peptide antibodiesODM-2Polymerase chain reaction amplificationChain reaction amplificationHuman ciliary epitheliumCell-specific distributionPattern of expression
1994
Nucleotide sequence of a cDNA for the β2 subunit isoform of Na+, K+-ATPase from human retina
Hernando N, Martin-Vasallo P, Ghosh S, Ghosh P, Swaroop A, Coca-Prados M. Nucleotide sequence of a cDNA for the β2 subunit isoform of Na+, K+-ATPase from human retina. Biochimica Et Biophysica Acta 1994, 1189: 109-111. PMID: 8305453, DOI: 10.1016/0005-2736(94)90287-9.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsBase SequenceDNA, ComplementaryHumansMolecular Sequence DataRetinaSodium-Potassium-Exchanging ATPaseInhibition of Na(+)-K(+)-ATPase activates Na(+)-K(+)-2Cl- cotransporter activity in cultured ciliary epithelium
Dong J, Delamere N, Coca-Prados M. Inhibition of Na(+)-K(+)-ATPase activates Na(+)-K(+)-2Cl- cotransporter activity in cultured ciliary epithelium. American Journal Of Physiology 1994, 266: c198-c205. PMID: 8304416, DOI: 10.1152/ajpcell.1994.266.1.c198.Peer-Reviewed Original ResearchConceptsCotransporter activityCiliary epitheliumATPase inhibitionPotassium channel blockerNonpigmented ciliary epitheliumChannel blockersOuabain treatmentStimulatory effectPotassium effluxExtracellular concentrationCellular sodiumPotassium channelsCellular potassiumOuabain-induced activationIntracellular potassiumPotassium influxEpitheliumCell linesCotransporter stimulationCotransporterInhibitionPotassium efflux rateEfflux rateATPase activityUptake component
1993
Calcium-dependent regulation of cation transport in cultured human nonpigmented ciliary epithelial cells
Mito T, Delamere N, Coca-Prados M. Calcium-dependent regulation of cation transport in cultured human nonpigmented ciliary epithelial cells. American Journal Of Physiology 1993, 264: c519-c526. PMID: 8384781, DOI: 10.1152/ajpcell.1993.264.3.c519.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportBumetanideCalcimycinCalciumCarrier ProteinsCationsCell Line, TransformedCiliary BodyEgtazic AcidEpithelial CellsEpitheliumHumansOuabainPigment Epithelium of EyePotassium ChannelsQuinidineRubidiumSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseTerpenesThapsigarginConceptsPotassium channelsIntracellular calcium bufferPotassium channel activityOuabain-sensitive potassium (86Rb) uptakeOuabain-sensitive componentCalcium-induced activationOuabain-insensitive componentNa-K-ATPaseCalcium-induced stimulationCiliary epithelial cellsNa-K-adenosinetriphosphataseMM quinidineThapsigargin stimulationIntracellular calciumPotassium lossCiliary epitheliumCalcium-dependent regulationEpithelial cellsAcetoxymethyl esterCalcium bufferCultured humanChannel activityCell linesCotransporterBumetanide
1991
Expression of multiple Na+,K+‐ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion
Ghosh S, Hernando N, Martín‐Alonso J, Martin‐Vasallo P, Coca‐Prados M. Expression of multiple Na+,K+‐ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion. Journal Of Cellular Physiology 1991, 149: 184-194. PMID: 1660898, DOI: 10.1002/jcp.1041490203.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernBlotting, WesternCattleCell MembraneCiliary BodyEpitheliumGene ExpressionMicroscopy, FluorescenceModels, BiologicalRNA, MessengerSodium-Potassium-Exchanging ATPase
1990
Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium.
Ghosh S, Freitag A, Martin-Vasallo P, Coca-Prados M. Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium. Journal Of Biological Chemistry 1990, 265: 2935-2940. PMID: 1689295, DOI: 10.1016/s0021-9258(19)39891-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCattleCell MembraneCell Transformation, ViralCiliary BodyFlow CytometryFluorescent Antibody TechniqueGene ExpressionIsoenzymesMacromolecular SubstancesMicroscopy, ElectronPigment Epithelium of EyeRatsRNASimian virus 40Sodium-Potassium-Exchanging ATPaseConceptsAlpha-subunit isoformsAlpha isoformSubunit isoformsK-ATPaseBasolateral plasma membrane domainsK-ATPase alpha subunitPlasma membrane domainsImmunoreactive signalPE cellsDifferential gene expressionAlpha 3Alpha 3 polypeptidesNPE cellsCell linesAlpha 2Alpha 1 polypeptideAlpha 1Pattern of expressionAlpha 3 isoformAlpha 1 isoformMembrane domainsSpecific cDNA probesSimian virus 40Differential immunostaining patternAlpha 2 isoform
1989
Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells
Martin‐Vasallo P, Ghosh S, Coca‐Prados M. Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells. Journal Of Cellular Physiology 1989, 141: 243-252. PMID: 2553750, DOI: 10.1002/jcp.1041410203.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCell LineCells, CulturedCiliary BodyHumansImmunohistochemistryIsoenzymesMicroscopy, ElectronPigment Epithelium of EyeRNA, MessengerSodium-Potassium-Exchanging ATPaseConceptsK-ATPase alpha subunit isoformsAlpha-subunit isoformsSubunit isoformsCultured cellsK-ATPase alpha subunitPE cellsExpression of NaBasolateral membrane domainsNorthern hybridization analysisNPE cell linesAlpha 2 mRNACell linesAlpha 3 isoformMembrane domainsAlpha 3 mRNAAlpha 1Alpha subunitCultured ciliary epithelial cellsWestern blot analysisHybridization analysisIsoformsCiliary epithelial cellsNPE layerBlot analysisK-ATPase
1987
Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye
Coca-Prados M, López-Briones L. Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye. Biochemical And Biophysical Research Communications 1987, 145: 460-466. PMID: 3036129, DOI: 10.1016/0006-291x(87)91343-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCattleCell MembraneCells, CulturedCiliary BodyEpitheliumHistocytochemistryIsoenzymesLamininMacromolecular SubstancesSodium-Potassium-Exchanging ATPaseConceptsCatalytic subunitCiliary epithelial cellsEpithelial cellsNPE cellsCultured ciliary epithelial cellsBasolateral surfaceImmunoblot analysisCiliary processesIntact ciliary processesMammalian eyeATPasePE cellsSubunitsPolyclonal antibodiesCanine kidneyCellsAlpha formAlphaIndirect immunofluorescenceIsoformsProteinBovine eyes