2005
The Cross-Species A3 Adenosine-Receptor Antagonist MRS 1292 Inhibits Adenosine-Triggered Human Nonpigmented Ciliary Epithelial Cell Fluid Release and Reduces Mouse Intraocular Pressure
Yang H, Avila MY, Peterson-Yantorno K, Coca-Prados M, Stone RA, Jacobson KA, Civan MM. The Cross-Species A3 Adenosine-Receptor Antagonist MRS 1292 Inhibits Adenosine-Triggered Human Nonpigmented Ciliary Epithelial Cell Fluid Release and Reduces Mouse Intraocular Pressure. Current Eye Research 2005, 30: 747-754. PMID: 16146920, PMCID: PMC3471215, DOI: 10.1080/02713680590953147.Peer-Reviewed Original Research
2004
Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells
Al-Nakkash L, Iserovich P, Coca-Prados M, Yang H, Reinach P. Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells. The Journal Of Membrane Biology 2004, 201: 41-49. PMID: 15635811, DOI: 10.1007/s00232-004-0706-5.Peer-Reviewed Original ResearchConceptsCorneal epithelial cellsRabbit corneal epithelial cellsVolume-regulated anion channelsEpithelial cellsVoltage-dependent inhibitionHypotonic challengeVolume-activated chloride channelsRegulatory volume decrease (RVD) responseInactive phorbol ester analoguePresence of DIDSExtracellular calciumChloride currentsNiflumic acidRVD responseCell swellingDecrease responseNorthern blot analysisBlot analysisPhorbol dibutyratePhorbol ester analogsChloride channelsVACCNPPBMOsm mediumSV40-immortalized rabbit corneal epithelial cells
2002
Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium
Cullinane A, Leung P, Ortego J, Coca-Prados M, Harvey B. Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium. British Journal Of Ophthalmology 2002, 86: 676. PMID: 12034692, PMCID: PMC1771149, DOI: 10.1136/bjo.86.6.676.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IICalciumCell SizeCells, CulturedCiliary BodyElectrophysiologyHumansLarge-Conductance Calcium-Activated Potassium ChannelsPatch-Clamp TechniquesPigment Epithelium of EyePotassium Channels, Calcium-ActivatedReceptors, AngiotensinRenin-Angiotensin SystemReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsHNPE cellsAngiotensin IINon-pigmented epitheliumSecretory functionAddition of AIIRenin-angiotensin system componentsRenin-angiotensin system expressionIon channel activityPotassium ion channel activityActive BK channelsExpression of angiotensinogenAqueous humor dynamicsTreatment of glaucomaChannel activitySingle-channel patch-clamp techniquesPatch-clamp techniqueBK channel activationLarge-conductance potassium channelsHuman ciliary bodyPolymerase chain reaction analysisReceptor antagonistCurrent armamentariumCiliary bodyChain reaction analysisRAS expression
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMP
2000
Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells
Mitchell C, Peterson‐Yantorno K, Coca‐Prados M, Civan M. Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells. The Journal Of Physiology 2000, 525: 183-193. PMID: 10811736, PMCID: PMC2269939, DOI: 10.1111/j.1469-7793.2000.00183.x.Peer-Reviewed Original Research
1998
Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume
Reeves R, Sanchez-Torres J, Coca-Prados M, Cammarata P. Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume. Current Eye Research 1998, 17: 861-869. PMID: 9746432, DOI: 10.1076/ceyr.17.9.861.5140.Peer-Reviewed Original ResearchConceptsBovine lens epithelial cellsCultured bovine lens epithelial cellsLens epithelial cellsHypertonic mediumChannel regulatory proteinRapid cellular swellingEpithelial cellsNorthern blot analysisTranscriptional regulationMyo-inositol effluxRegulatory proteinsMRNA expressionAnion channelCellular shrinkageCell volume changesCell volumeBlot analysisCell volume reductionExpression levelsMRNAChloride movementExpressionCellsMedium conditionsCellular swelling
1996
Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells
CIVAN M, COCA-PRADOS M, PETERSON-YANTORNO K. Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells. Experimental Eye Research 1996, 62: 627-640. PMID: 8983945, DOI: 10.1006/exer.1996.0074.Peer-Reviewed Original ResearchConceptsRegulatory volume increaseCiliary epithelial cellsEpithelial cellsNon-pigmented ciliary epithelial cellsPKC inhibitor staurosporineHuman non-pigmented ciliary epithelial cellsElectronic cell sizingRegulatory volume responsesAcid metabolismSame cellsTransport inhibitorsStaurosporineHuman NonSymport
1994
Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells.
Civan M, Coca-Prados M, Peterson-Yantorno K. Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1994, 35: 2876-86. PMID: 8188483.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseNonpigmented ciliary epithelial cellsProtein phosphatase inhibitor okadaic acidCiliary epithelial cellsPhosphatase inhibitor okadaic acidCultured nonpigmented ciliary epithelial cellsInhibitor okadaic acidEpithelial cellsNPE cellsHuman nonpigmented ciliary epithelial cellsCl- channelsCyclic AMPPKC inhibitor staurosporineEndogenous PKC activityOkadaic acidRegulatory responsesIntracellular cAMP concentrationCell typesPKC activatorCalcium-calmodulinPKC activityCell sortingElectronic cell sortingPathwayStaurosporine