2011
Interaction of Recombinant Myocilin with the Matricellular Protein SPARC: Functional Implications
Aroca-Aguilar JD, Sánchez-Sánchez F, Ghosh S, Fernández-Navarro A, Coca-Prados M, Escribano J. Interaction of Recombinant Myocilin with the Matricellular Protein SPARC: Functional Implications. Investigative Ophthalmology & Visual Science 2011, 52: 179-189. PMID: 20926826, PMCID: PMC3053273, DOI: 10.1167/iovs.09-4866.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBlotting, WesternCalcium-Binding ProteinsCell LineChromatography, High Pressure LiquidCytoskeletal ProteinsExtracellular Matrix ProteinsEye ProteinsGene Expression ProfilingGlycoproteinsHumansKidneyMicroscopy, FluorescenceMolecular Sequence DataOsteonectinPolymerase Chain ReactionProtein BindingRecombinant Fusion ProteinsRecombinant ProteinsTwo-Hybrid System TechniquesConceptsHEK 293T cellsSolid-phase binding assaysInteraction of myocilinRecombinant proteinsRecombinant myocilinMatricellular proteinEC domainTerminal olfactomedin domainTwo-hybrid analysisTwo-hybrid systemProtein-protein interactionsFull-length myocilinC-terminal domainN-terminal domainC-terminal regionCalcium binding domainsBinding assaysOlfactomedin domainC-terminal fragmentBinding domainsLinker regionUnknown functionExtracellular glycoproteinIntracellular interactionsSPARC family
2009
Functional Role of Proteolytic Processing of Recombinant Myocilin in Self-Aggregation
Aroca-Aguilar JD, Martínez-Redondo F, Sánchez-Sánchez F, Coca-Prados M, Escribano J. Functional Role of Proteolytic Processing of Recombinant Myocilin in Self-Aggregation. Investigative Ophthalmology & Visual Science 2009, 51: 72-78. PMID: 19696176, PMCID: PMC2869055, DOI: 10.1167/iovs.09-4118.Peer-Reviewed Original Research
2005
Interaction of myocilin with the C-terminal region of hevin
Li Y, Aroca-Aguilar JD, Ghosh S, Sánchez-Sánchez F, Escribano J, Coca-Prados M. Interaction of myocilin with the C-terminal region of hevin. Biochemical And Biophysical Research Communications 2005, 339: 797-804. PMID: 16316624, DOI: 10.1016/j.bbrc.2005.11.082.Peer-Reviewed Original ResearchFOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner
Berry FB, O'Neill MA, Coca-Prados M, Walter MA. FOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner. Molecular And Cellular Biology 2005, 25: 1415-1424. PMID: 15684392, PMCID: PMC548007, DOI: 10.1128/mcb.25.4.1415-1424.2005.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationCell NucleusChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneContractile ProteinsDNA-Binding ProteinsEye AbnormalitiesFilaminsForkhead Transcription FactorsHeLa CellsHeterochromatinHumansMicrofilament ProteinsMutationProtein BindingProto-Oncogene ProteinsTranscription FactorsTumor Cells, CulturedConceptsFilamin AHuman nonpigmented ciliary epithelial cellsFunction mutationsNonpigmented ciliary epithelial cellsAxenfeld-Rieger syndromeAutosomal dominant disorderCiliary epithelial cellsMelanoma cellsSkeletal disordersElevated levelsDominant disorderEpithelial cellsImportant transcription factorSkeletal phenotypeProtein filamin AFOXC1Inhibitory activityDisorders