1990
Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium.
Ghosh S, Freitag A, Martin-Vasallo P, Coca-Prados M. Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium. Journal Of Biological Chemistry 1990, 265: 2935-2940. PMID: 1689295, DOI: 10.1016/s0021-9258(19)39891-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCattleCell MembraneCell Transformation, ViralCiliary BodyFlow CytometryFluorescent Antibody TechniqueGene ExpressionIsoenzymesMacromolecular SubstancesMicroscopy, ElectronPigment Epithelium of EyeRatsRNASimian virus 40Sodium-Potassium-Exchanging ATPaseConceptsAlpha-subunit isoformsAlpha isoformSubunit isoformsK-ATPaseBasolateral plasma membrane domainsK-ATPase alpha subunitPlasma membrane domainsImmunoreactive signalPE cellsDifferential gene expressionAlpha 3Alpha 3 polypeptidesNPE cellsCell linesAlpha 2Alpha 1 polypeptideAlpha 1Pattern of expressionAlpha 3 isoformAlpha 1 isoformMembrane domainsSpecific cDNA probesSimian virus 40Differential immunostaining patternAlpha 2 isoform
1986
Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.
Coca-Prados M, Wax M. Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 8754-8758. PMID: 3022303, PMCID: PMC387010, DOI: 10.1073/pnas.83.22.8754.Peer-Reviewed Original ResearchConceptsSimian virus 40Ciliary epithelial cellsSouthern blot analysisLarge tumor antigenHuman ciliary epithelial cellsEpithelial cellsFunctional conservationProtein phosphorylationDirect bindingCell DNAParent cellsBlot analysisCell proliferationCellular cAMP productionReceptor-mediated processEpithelial cell DNAViral DNAImmunoprecipitationDNACAMP productionSelective drugsCell clonesMembrane preparationsCellsReceptors
1980
Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA.
Ford J, Coca-Prados M, Hsu M. Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA. Journal Of Biological Chemistry 1980, 255: 7544-7547. PMID: 6249806, DOI: 10.1016/s0021-9258(19)43861-1.Peer-Reviewed Original ResearchConceptsEukaryotic gene expressionSimian virus 40 DNAEukaryotic cellsEukaryotic DNASimian virus 40Gene expressionHost DNAViral genomeCell DNADNAMonkey kidney cellsIntracellular viral DNAEnzymatic analysisViral DNAKidney cellsGenomeCellsAcid hydrolysis procedureThymineExact quantitationDeaminationExpressionEnzymatic methodVivo