2018
The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells
Rodríguez-Menéndez S, García M, Fernández B, Álvarez L, Fernández-Vega-Cueto A, Coca-Prados M, Pereiro R, González-Iglesias H. The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients 2018, 10: 1874. PMID: 30513827, PMCID: PMC6315569, DOI: 10.3390/nu10121874.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumOxidative stressRetinal pigment epithelial cellsOxidative damagePre-treated cellsInduces oxidative stressPigment epithelial cellsNon-treated cellsReactive oxygen intermediatesΜM of zincPigment epitheliumRPE cellsZn-MTProtective mechanismEpithelial cellsFree radical generatorMT levelsAAPH treatmentOxygen intermediates
2017
Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies
Rodríguez-Menéndez S, Fernández B, García M, Álvarez L, Fernández M, Sanz-Medel A, Coca-Prados M, Pereiro R, González-Iglesias H. Quantitative study of zinc and metallothioneins in the human retina and RPE cells by mass spectrometry-based methodologies. Talanta 2017, 178: 222-230. PMID: 29136815, DOI: 10.1016/j.talanta.2017.09.024.Peer-Reviewed Original Research
2010
Neuroendocrine Properties of the Ciliary Epithelium
Coca-Prados M. Neuroendocrine Properties of the Ciliary Epithelium. 2010, 107-112. DOI: 10.1016/b978-0-12-374203-2.00088-9.Peer-Reviewed Original ResearchCiliary epitheliumAqueous humorOcular hypotensive actionSame embryological originRetinal pigment epitheliumEye aqueous humorOcular ciliary epitheliumHypotensive actionAqueous humor secretionAnterior segmentEndocrine factorsPigment epitheliumNeuroendocrine propertiesEndocrine systemEmbryological originNeuroepithelial cellsEpitheliumAvascular tissueNeuropeptidesBioactive peptidesCellsCell communicationRetinaHormoneSecretion
2003
CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells
Kennedy BN, Li C, Ortego J, Coca-Prados M, Sarthy VP, Crabb JW. CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells. Experimental Eye Research 2003, 76: 257-260. PMID: 12565814, DOI: 10.1016/s0014-4835(02)00308-1.Peer-Reviewed Original ResearchConceptsTranscriptional regulationBinding Protein FunctionsCell-specific expressionPromoter analysisProtein functionRepressor elementEnhancer elementsPromoter constructsReporter activityRetinal pigment epithelial cellsPigment epithelial cellsCiliary epitheliumEpithelial cellsVisual cycleRPE cellsBPRegulationRetinal pigment epitheliumCellsMüller cellsGenesMutationsRLBP1 geneEpitheliumWildtype
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor