2003
Expression of cell surface transmembrane carbonic anhydrase genes CA9 and CA12 in the human eye: overexpression of CA12 (CAXII) in glaucoma
Liao S, Ivanov S, Ivanova A, Ghosh S, Cote M, Keefe K, Coca-Prados M, Stanbridge E, Lerman M. Expression of cell surface transmembrane carbonic anhydrase genes CA9 and CA12 in the human eye: overexpression of CA12 (CAXII) in glaucoma. Journal Of Medical Genetics 2003, 40: 257. PMID: 12676895, PMCID: PMC1735430, DOI: 10.1136/jmg.40.4.257.Peer-Reviewed Original ResearchConceptsNon-pigmented ciliary epithelial cellsGlaucoma patientsEpithelium of corneaNPE cell linesNPE cellsAqueous humor productionCultured NPE cellsGlaucoma eyesIntraocular pressureAqueous humor secretionGlaucomatous eyesCAIX expressionCiliary epithelial cellsCAXII expressionEye donorsCA9 mRNANormal eyesPlasma membrane proteinsEmbryonic eye developmentFundamental physiological processesGlaucoma donorsGlaucomaCiliary epitheliumCAIXEpithelial cells
1999
Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells
Shi C, Ryan J, French A, Coca‐Prados M, Kelly M. Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells. The Journal Of Physiology 1999, 521: 57-67. PMID: 10562334, PMCID: PMC2269649, DOI: 10.1111/j.1469-7793.1999.00057.x.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimalsCalciumCell LineChloride ChannelsCiliary BodyEnzyme InhibitorsEpithelial CellsNiflumic AcidOsmotic PressurePatch-Clamp TechniquesPhosphorylationProtein Kinase InhibitorsRabbitsSecond Messenger SystemsStilbenesConceptsNon-pigmented ciliary epithelial cellsWhole-cell currentsCiliary epithelial cellsWhole-cell patch-clamp recording techniquePatch-clamp recording techniquesEpithelial cellsTime courseSlower time courseDisulfonic acidSmall conductanceWhole-cell conductanceCl- current amplitudeExposure of cellsVoltage-sensitive blockCl- currentVoltage-independent blockNiflumic acidRecording techniquesDepolarized potentialsCurrent activationAmplitude of currentsOutward rectificationCell swellingNPCE cellsHyposmotic shock
1996
Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells
CIVAN M, COCA-PRADOS M, PETERSON-YANTORNO K. Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells. Experimental Eye Research 1996, 62: 627-640. PMID: 8983945, DOI: 10.1006/exer.1996.0074.Peer-Reviewed Original ResearchConceptsRegulatory volume increaseCiliary epithelial cellsEpithelial cellsNon-pigmented ciliary epithelial cellsPKC inhibitor staurosporineHuman non-pigmented ciliary epithelial cellsElectronic cell sizingRegulatory volume responsesAcid metabolismSame cellsTransport inhibitorsStaurosporineHuman NonSymport
1991
Differential expression of HLA DR, DP and DQ in cultivated, human ciliary body epithelial cells.
Helbig H, Kittredge K, Coca-Prados M, Nussenblatt R. Differential expression of HLA DR, DP and DQ in cultivated, human ciliary body epithelial cells. Die Ophthalmologie 1991, 88: 295-8. PMID: 1889783.Peer-Reviewed Original ResearchConceptsHLA-DRMHC-IIGamma-IFNHLA-DQClass IIMajor histocompatibility complex class IIHistocompatibility complex class IICiliary epitheliumEpithelial cellsHuman non-pigmented ciliary epithelial cellsT helper lymphocytesAntigen-specific activationMHC-II expressionCultured human non-pigmented ciliary epithelial cellsMHC class IIMeans of immunohistochemistryPresentation of antigensHuman MHC IINon-pigmented ciliary epithelial cellsOcular autoimmunityUveitic eyesCiliary epithelial cellsCiliary body epithelial cellsLymphoid cellsDifferential expression
1986
Growth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media
Coca-Prados M, Chatt G. Growth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media. Experimental Eye Research 1986, 43: 617-629. PMID: 3792464, DOI: 10.1016/s0014-4835(86)80028-8.Peer-Reviewed Original ResearchConceptsNPE cellsClathrin-coated vesiclesCiliary epithelial cellsNerve growth factorEpithelial cellsNon-pigmented ciliary epithelial cellsCellular polarityGrowth factorCellular junctionsPrimary culturesCoated pitsArtificial substratesTransport activityCulture mediumMicrofilament bundlesCell growthSerum-free hormone-supplemented mediumJunctional complexesIntermediate filamentsCell proliferationAdjacent cellsCellsSerum-free culture medium