2001
Extracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Extracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 448-454. PMID: 12045895, DOI: 10.1076/ceyr.23.6.448.6964.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCalciumCalcium-Transporting ATPasesCells, CulturedCiliary BodyFluorescent DyesFura-2HumansMicroscopy, FluorescencePigment Epithelium of EyeReceptors, Purinergic P2Receptors, Purinergic P2Y2Signal TransductionSodium-Calcium ExchangerSpectrometry, FluorescenceThapsigarginConceptsNon-pigmented ciliary body epitheliumCiliary body epitheliumHNPE cellsIntracellular storesBody epitheliumFluorescent dye fura-2Extracellular ATP effectP2Y2 receptor activationDye fura-2Calcium signalsATPase pumpPresence of thapsigarginCapacitative calcium entrySodium-free solutionIntracellular calciumPurinergic receptorsFura-2Therapeutic targetCalcium entryPotency orderReceptor activationSustained increaseL-typeExtracellular ATPOscillatory increases
1989
Effect of acetylcholine on membrane potential of cultured human nonpigmented ciliary epithelial cells.
Helbig H, Korbmacher C, Wohlfarth J, Coroneo M, Lindschau C, Quass P, Haller H, Coca-Prados M, Wiederholt M. Effect of acetylcholine on membrane potential of cultured human nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1989, 30: 890-6. PMID: 2722445.Peer-Reviewed Original ResearchConceptsAcetylcholine-induced responsesCiliary epithelial cellsCytoplasmic Ca2Epithelial cellsEffects of acetylcholineAddition of acetylcholineMembrane potentialMobilization of Ca2M atropineRole of Ca2M acetylcholineMM quinidineChannel blockersFura-2Sustained depolarizationAcetylcholineIntracellular storesInitial hyperpolarizationTransient hyperpolarizationCell membrane potentialMicroelectrode techniquesCultured humanHyperpolarizationFree mediumOrigin-defective mutant