2013
MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels
Hotchkiss A, Pasumarthi K, Coca‐Prados M, Pelis R. MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels. The FASEB Journal 2013, 27: 732.3-732.3. DOI: 10.1096/fasebj.27.1_supplement.732.3.Peer-Reviewed Original ResearchMultidrug resistance-associated proteinCAMP levelsExtracellular cAMP levelsCiliary epitheliumAqueous humor dynamicsResistance-associated proteinCiliary epithelial cell lineHuman ciliary bodyCE cellsEpithelial cell lineMRP inhibitorsCiliary bodyAdrenergic stimulationIntracellular cAMPIntracellular accumulationPotential roleCell linesIndomethacinFurosemideMRP4Cyclic nucleotidesMRP5EpitheliumIbuprofenCellsBicarbonate-Dependent Secretion and Proteolytic Processing of Recombinant Myocilin
Aroca-Aguilar JD, Martínez-Redondo F, Martín-Gil A, Pintor J, Coca-Prados M, Escribano J. Bicarbonate-Dependent Secretion and Proteolytic Processing of Recombinant Myocilin. PLOS ONE 2013, 8: e54385. PMID: 23342144, PMCID: PMC3547000, DOI: 10.1371/journal.pone.0054385.Peer-Reviewed Original ResearchConceptsIrreversible visual lossElevated intraocular pressureIntracellular accumulationC-terminal fragmentVisual lossOptic neuropathyIntraocular pressureIntracellular proteolytic processingProteolytic processingOcular tissuesRecombinant myocilinCalpain IICellular modelMyocilinSecretionPossible factorsExtracellular glycoproteinNeuropathyGlaucoma
2012
Functional Expression of MRP4 in the Ciliary Epithelium of the Eye
Pelis R, Coca-Prados M. Functional Expression of MRP4 in the Ciliary Epithelium of the Eye. The FASEB Journal 2012, 26: 694.10-694.10. DOI: 10.1096/fasebj.26.1_supplement.694.10.Peer-Reviewed Original ResearchFunctional expressionOrganic anion transportCiliary epitheliumAnion transportPara-aminohippurateMRP4 protein levelsCiliary body epithelial cellsHuman ciliary epitheliumCultured cellsFunctional assaysProtein 4Dose-dependent mannerProtein levelsCell linesEfflux pumpsHuman ciliary bodyUseful systemSimian virusEpithelial cellsWestern blottingIntracellular accumulationCiliary bodyMrp4 mRNAMRP4 expressionExpression
2009
Localization of Multidrug Resistance-Associated Protein 2 in the Nonpigmented Ciliary Epithelium of the Eye
Pelis RM, Shahidullah M, Ghosh S, Coca-Prados M, Wright SH, Delamere NA. Localization of Multidrug Resistance-Associated Protein 2 in the Nonpigmented Ciliary Epithelium of the Eye. Journal Of Pharmacology And Experimental Therapeutics 2009, 329: 479-485. PMID: 19201990, PMCID: PMC2672870, DOI: 10.1124/jpet.108.149625.Peer-Reviewed Original ResearchConceptsNonpigmented epitheliumMicroM MK571Ciliary bodyMultidrug resistance associated protein 2Therapeutic drugsMrp2 proteinIntracellular accumulationBlood-aqueous barrierNonpigmented ciliary epitheliumProtein 2Human ciliary bodyMicroM indomethacinMRP inhibitorsIntraocular tissuesAqueous humorApical membraneMRP2 mRNAWestern blotMultidrug resistanceCiliary epitheliumMicroM cyclosporinPorcine eyesMRP2Cell layerNative human