2013
MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels
Hotchkiss A, Pasumarthi K, Coca‐Prados M, Pelis R. MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels. The FASEB Journal 2013, 27: 732.3-732.3. DOI: 10.1096/fasebj.27.1_supplement.732.3.Peer-Reviewed Original ResearchMultidrug resistance-associated proteinCAMP levelsExtracellular cAMP levelsCiliary epitheliumAqueous humor dynamicsResistance-associated proteinCiliary epithelial cell lineHuman ciliary bodyCE cellsEpithelial cell lineMRP inhibitorsCiliary bodyAdrenergic stimulationIntracellular cAMPIntracellular accumulationPotential roleCell linesIndomethacinFurosemideMRP4Cyclic nucleotidesMRP5EpitheliumIbuprofenCells
2010
Neuroendocrine Properties of the Ciliary Epithelium
Coca-Prados M. Neuroendocrine Properties of the Ciliary Epithelium. 2010, 107-112. DOI: 10.1016/b978-0-12-374203-2.00088-9.Peer-Reviewed Original ResearchCiliary epitheliumAqueous humorOcular hypotensive actionSame embryological originRetinal pigment epitheliumEye aqueous humorOcular ciliary epitheliumHypotensive actionAqueous humor secretionAnterior segmentEndocrine factorsPigment epitheliumNeuroendocrine propertiesEndocrine systemEmbryological originNeuroepithelial cellsEpitheliumAvascular tissueNeuropeptidesBioactive peptidesCellsCell communicationRetinaHormoneSecretion
2006
Somatostatin modulates PI3K-Akt, eNOS and NHE activity in the ciliary epithelium
Ghosh S, Choritz L, Geibel J, Coca-Prados M. Somatostatin modulates PI3K-Akt, eNOS and NHE activity in the ciliary epithelium. Molecular And Cellular Endocrinology 2006, 253: 63-75. PMID: 16764985, DOI: 10.1016/j.mce.2006.05.002.Peer-Reviewed Original ResearchConceptsP-Akt Ser473Ciliary epitheliumIntracellular cyclic GMP productionInvolvement of somatostatinDose-dependent attenuationReceptor mRNA expressionCyclic GMP productionSST-like immunoreactivityLack of responseOcular ciliary epitheliumAqueous humorSomatostatinNHE activityExchanger activityMRNA expressionCiliary processesNeuroendocrine cellsPC2 antibodyPI3K-AktMultiple intracellularGMP productionConvertases PC1Increases phosphorylationTissue extractsEpithelium
2004
The bovine iris–ciliary epithelium expresses components of rod phototransduction
Ghosh S, Salvador-Silva M, Coca-Prados M. The bovine iris–ciliary epithelium expresses components of rod phototransduction. Neuroscience Letters 2004, 370: 7-12. PMID: 15489008, DOI: 10.1016/j.neulet.2004.07.026.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornArrestinBlotting, NorthernBlotting, WesternCattleCiliary BodyEpitheliumEye ProteinsGene ExpressionG-Protein-Coupled Receptor Kinase 1IrisLight Signal TransductionNeuronsPromoter Regions, GeneticProtein KinasesRetinal Rod Photoreceptor CellsReverse Transcriptase Polymerase Chain ReactionRhodopsinRNA, MessengerTransfectionConceptsCiliary epitheliumIris cellsBasal activityCommon embryonic originOcular ciliary epitheliumNeural retinaWestern blotRT-PCR amplificationRetinaEpitheliumSignificant stimulationBovine irisEmbryonic originStimulationBlotRod phototransductionTransient transfectionNorthern blotRhodopsin kinasePromoter activityDistal promoter elementLow levelsIrisReporter constructsLower vertebrates
2003
Sex steroid hormone metabolism takes place in human ocular cells
Coca-Prados M, Ghosh S, Wang Y, Escribano J, Herrala A, Vihko P. Sex steroid hormone metabolism takes place in human ocular cells. The Journal Of Steroid Biochemistry And Molecular Biology 2003, 86: 207-216. PMID: 14568574, DOI: 10.1016/j.jsbmb.2003.08.001.Peer-Reviewed Original ResearchConceptsSex steroid hormone metabolismCiliary epitheliumHuman ocular cellsSex steroid hormonesHuman ciliary epithelial cellsSteroid hormone metabolismAndrogen agonistsCiliary epithelial cellsEstrogenic milieuOcular diseasesOcular cellsSteroid hormonesMRNA expressionImportant mediatorOxidative enzymatic activityHuman ciliary epitheliumGene expressionEpithelial cellsHormone metabolismHormone-responsive genesSecretory epitheliumEpitheliumH time courseCell linesTime courseCRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells
Kennedy BN, Li C, Ortego J, Coca-Prados M, Sarthy VP, Crabb JW. CRALBP transcriptional regulation in ciliary epithelial, retinal Müller and retinal pigment epithelial cells. Experimental Eye Research 2003, 76: 257-260. PMID: 12565814, DOI: 10.1016/s0014-4835(02)00308-1.Peer-Reviewed Original ResearchConceptsTranscriptional regulationBinding Protein FunctionsCell-specific expressionPromoter analysisProtein functionRepressor elementEnhancer elementsPromoter constructsReporter activityRetinal pigment epithelial cellsPigment epithelial cellsCiliary epitheliumEpithelial cellsVisual cycleRPE cellsBPRegulationRetinal pigment epitheliumCellsMüller cellsGenesMutationsRLBP1 geneEpitheliumWildtype
2002
Adult-Onset Primary Open-Angle Glaucoma Caused by Mutations in Optineurin
Rezaie T, Child A, Hitchings R, Brice G, Miller L, Coca-Prados M, Héon E, Krupin T, Ritch R, Kreutzer D, Crick RP, Sarfarazi M. Adult-Onset Primary Open-Angle Glaucoma Caused by Mutations in Optineurin. Science 2002, 295: 1077-1079. PMID: 11834836, DOI: 10.1126/science.1066901.Peer-Reviewed Original ResearchMeSH KeywordsAdultAlternative SplicingAmino Acid SequenceBrainCell Cycle ProteinsChromosome MappingChromosomes, Human, Pair 10Ciliary BodyExonsEye ProteinsFemaleGlaucoma, Open-AngleGolgi ApparatusHeterozygoteHumansIntraocular PressureMaleMembrane Transport ProteinsMiddle AgedMutationMutation, MissenseNerve Tissue ProteinsOcular HypertensionPedigreePolymorphism, Single-Stranded ConformationalRetinaTrabecular MeshworkTranscription Factor TFIIIAZinc FingersConceptsPrimary open-angle glaucomaHereditary primary open-angle glaucomaNormal intraocular pressureOpen-angle glaucomaAdult-onset primary open-angle glaucomaNeuroprotective roleIntraocular pressureAngle glaucomaTumor necrosisLeading causeTrabecular meshworkOPTN geneCiliary epitheliumCausative genesGlaucomaChromosome 10p14OptineurinSequence alterationsNecrosisRetinaIndividualsBrainEpithelium
2001
Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction
Bertazolli-Filho R, Ghosh S, Huang W, Wollmann G, Coca-Prados M. Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction. Biochemical And Biophysical Research Communications 2001, 284: 317-325. PMID: 11394879, DOI: 10.1006/bbrc.2001.4970.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArrestinBlotting, WesternCalcium-Binding ProteinsCattleCell LineCiliary BodyCyclic Nucleotide-Gated Cation ChannelsEpithelial CellsEye ProteinsFluorescent Antibody Technique, IndirectG-Protein-Coupled Receptor Kinase 1HippocalcinHumansImmunoblottingImmunohistochemistryIon ChannelsLight Signal TransductionLipoproteinsNerve Tissue ProteinsPhosphoric Diester HydrolasesProtein KinasesProtein SubunitsRecoverinRetinaReverse Transcriptase Polymerase Chain ReactionRhodopsinRNA, MessengerSequence Analysis, DNATransducinConceptsHuman ocular ciliary epitheliumCiliary epitheliumOcular ciliary epitheliumNPE cellsCiliary epithelial cell linePars plicata regionIntact ciliary epitheliumWestern blot analysisCultured NPE cellsEpithelial cell lineExpression of rhodopsinIndirect immunofluorescenceVisual arrestinHuman retinaMonoclonal antibodiesHuman ciliary epitheliumThree- to fourfoldEpitheliumRT-PCRPhotoreceptor cellsRhodopsin mRNABlot analysisCell linesRetinaRhodopsin kinaseChloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Chloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 443-447. PMID: 12045894, DOI: 10.1076/ceyr.23.6.443.6967.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnti-Bacterial AgentsCalciumCells, CulturedChloride-Bicarbonate AntiportersChloridesCiliary BodyFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationMacrolidesMicroscopy, FluorescencePigment Epithelium of EyeProton-Translocating ATPasesReceptors, PurinergicSpectrometry, FluorescenceSuraminUp-RegulationConceptsNon-pigmented ciliary body epitheliumHNPE cellsCiliary body epitheliumAcetoxymethyl ester formBody epitheliumIntracellular calcium levelsCell-permeable calcium chelatorFluorescent probe BCECFReceptor inhibitorsIntracellular calciumPurinergic receptorsCalcium levelsDiisothiocyanatostilbene-2Extracellular adenosineSustained increaseMM suraminInhibitor amilorideExtracellular ATPCalcium chelatorEster formMicroM ATPATPase inhibitorEpitheliumInhibitorsIntracellular
1994
Inhibition of Na(+)-K(+)-ATPase activates Na(+)-K(+)-2Cl- cotransporter activity in cultured ciliary epithelium
Dong J, Delamere N, Coca-Prados M. Inhibition of Na(+)-K(+)-ATPase activates Na(+)-K(+)-2Cl- cotransporter activity in cultured ciliary epithelium. American Journal Of Physiology 1994, 266: c198-c205. PMID: 8304416, DOI: 10.1152/ajpcell.1994.266.1.c198.Peer-Reviewed Original ResearchConceptsCotransporter activityCiliary epitheliumATPase inhibitionPotassium channel blockerNonpigmented ciliary epitheliumChannel blockersOuabain treatmentStimulatory effectPotassium effluxExtracellular concentrationCellular sodiumPotassium channelsCellular potassiumOuabain-induced activationIntracellular potassiumPotassium influxEpitheliumCell linesCotransporter stimulationCotransporterInhibitionPotassium efflux rateEfflux rateATPase activityUptake component
1992
Isolation of a cDNA encoding a glutathione S-transferase (GST) class-π from the bovine ocular ciliary epithelium
Hernando N, Martin-Alonso J, Ghosh S, Coca-Prados M. Isolation of a cDNA encoding a glutathione S-transferase (GST) class-π from the bovine ocular ciliary epithelium. Experimental Eye Research 1992, 55: 711-718. PMID: 1478280, DOI: 10.1016/0014-4835(92)90175-r.Peer-Reviewed Original Research
1991
Mammalian ciliary-body epithelial cells in culture produce transforming growth factor-beta
Helbig H, Kittredge K, Coca-Prados M, Davis J, Palestine A, Nussenblatt R. Mammalian ciliary-body epithelial cells in culture produce transforming growth factor-beta. Graefe's Archive For Clinical And Experimental Ophthalmology 1991, 229: 84-87. PMID: 2004731, DOI: 10.1007/bf00172268.Peer-Reviewed Original Research
1989
Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium.
Helbig H, Korbmacher C, Wohlfarth J, Coca-Prados M, Wiederholt M. Electrical membrane properties of a cell clone derived from human nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1989, 30: 882-9. PMID: 2470692.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidCalciumCell MembraneChloridesCiliary BodyClone CellsCyclamatesElectric ConductivityEpithelial CellsEpitheliumExtracellular SpaceHumansHydrogen-Ion ConcentrationOuabainPigmentationPotassiumSodiumConceptsIntracellular potentialsCiliary epitheliumCell clonesPresence of SITSPresence of ouabainElectrical membrane propertiesAddition of ouabainCiliary epithelial cellsMM EGTAEpithelial cellsTransient depolarizationMicroelectrode techniquesMembrane voltageCultured humanExtracellular ClCl- conductanceEpitheliumOuabainMembrane potentialIntracellular voltageFree solutionPotential response
1988
Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium
Helbig H, Korbmacher C, Stumpff F, Coca‐Prados M, Wiederholt M. Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium. Journal Of Cellular Physiology 1988, 137: 384-389. PMID: 2848042, DOI: 10.1002/jcp.1041370225.Peer-Reviewed Original ResearchConceptsPigmented ciliary epitheliumMM amilorideAcid loadCiliary epitheliumCell clonesPH-sensitive absorbanceBicarbonate-free mediumRegulation of intracellularCiliary bodyAmilorideExtracellular protonsBovine ciliary bodyEpitheliumConfluent monolayersIntracellularCellsCellular metabolismSlow acidificationAlkalinizationPlastic coverslips