2003
Expression of cell surface transmembrane carbonic anhydrase genes CA9 and CA12 in the human eye: overexpression of CA12 (CAXII) in glaucoma
Liao S, Ivanov S, Ivanova A, Ghosh S, Cote M, Keefe K, Coca-Prados M, Stanbridge E, Lerman M. Expression of cell surface transmembrane carbonic anhydrase genes CA9 and CA12 in the human eye: overexpression of CA12 (CAXII) in glaucoma. Journal Of Medical Genetics 2003, 40: 257. PMID: 12676895, PMCID: PMC1735430, DOI: 10.1136/jmg.40.4.257.Peer-Reviewed Original ResearchConceptsNon-pigmented ciliary epithelial cellsGlaucoma patientsEpithelium of corneaNPE cell linesNPE cellsAqueous humor productionCultured NPE cellsGlaucoma eyesIntraocular pressureAqueous humor secretionGlaucomatous eyesCAIX expressionCiliary epithelial cellsCAXII expressionEye donorsCA9 mRNANormal eyesPlasma membrane proteinsEmbryonic eye developmentFundamental physiological processesGlaucoma donorsGlaucomaCiliary epitheliumCAIXEpithelial cells
2001
Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction
Bertazolli-Filho R, Ghosh S, Huang W, Wollmann G, Coca-Prados M. Molecular Evidence That Human Ocular Ciliary Epithelium Expresses Components Involved in Phototransduction. Biochemical And Biophysical Research Communications 2001, 284: 317-325. PMID: 11394879, DOI: 10.1006/bbrc.2001.4970.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArrestinBlotting, WesternCalcium-Binding ProteinsCattleCell LineCiliary BodyCyclic Nucleotide-Gated Cation ChannelsEpithelial CellsEye ProteinsFluorescent Antibody Technique, IndirectG-Protein-Coupled Receptor Kinase 1HippocalcinHumansImmunoblottingImmunohistochemistryIon ChannelsLight Signal TransductionLipoproteinsNerve Tissue ProteinsPhosphoric Diester HydrolasesProtein KinasesProtein SubunitsRecoverinRetinaReverse Transcriptase Polymerase Chain ReactionRhodopsinRNA, MessengerSequence Analysis, DNATransducinConceptsHuman ocular ciliary epitheliumCiliary epitheliumOcular ciliary epitheliumNPE cellsCiliary epithelial cell linePars plicata regionIntact ciliary epitheliumWestern blot analysisCultured NPE cellsEpithelial cell lineExpression of rhodopsinIndirect immunofluorescenceVisual arrestinHuman retinaMonoclonal antibodiesHuman ciliary epitheliumThree- to fourfoldEpitheliumRT-PCRPhotoreceptor cellsRhodopsin mRNABlot analysisCell linesRetinaRhodopsin kinase
1999
Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells
Ortego J, Coca-Prados M. Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells. Biochemical And Biophysical Research Communications 1999, 258: 21-28. PMID: 10222228, DOI: 10.1006/bbrc.1999.0573.Peer-Reviewed Original ResearchConceptsNatriuretic peptide systemNPR-B receptorNPR-C receptorCiliary epithelial cellsReceptor mRNACiliary epitheliumEpithelial cellsNPE cellsCGMP responseAutocrine/paracrine modulatorPeptide systemHuman ocular ciliary epitheliumNonpigmented ciliary epithelial cellsOcular ciliary epitheliumCultured NPE cellsLong-term downregulationC-ANP4Paracrine modulatorEye pressureBNPActivation of PKCBasal levelsInhibitory effectRT-PCRReceptors
1995
PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln
Coca-Prados M, Anguita J, Chalfant M, Civan M. PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln. American Journal Of Physiology 1995, 268: c572-c579. PMID: 7534980, DOI: 10.1152/ajpcell.1995.268.3.c572.Peer-Reviewed Original ResearchMeSH KeywordsAlkaloidsAnimalsBase SequenceBlotting, NorthernCells, CulturedChild, PreschoolChloride ChannelsCiliaCystic Fibrosis Transmembrane Conductance RegulatorElectric ConductivityEpitheliumHumansIon ChannelsKineticsMembrane ProteinsMolecular Sequence DataPolymerase Chain ReactionProtein Kinase CRatsRNA, MessengerStaurosporine