2016
Occurrence and molecular characterization of Sapelovirus A in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm
BAK G, KANG M, SON K, PARK J, KIM D, SEO J, KIM J, ALFAJARO M, SOLIMAN M, BAEK Y, CHO E, Joseph K, CHOI J, PARK S, CHO K. Occurrence and molecular characterization of Sapelovirus A in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm. Journal Of Veterinary Medical Science 2016, 78: 16-0237. PMID: 27616554, PMCID: PMC5240775, DOI: 10.1292/jvms.16-0237.Peer-Reviewed Original Research
2014
Anti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract
Alfajaro M, Rho M, Kim H, Park J, Kim D, Hosmillo M, Son K, Lee J, Park S, Kang M, Ryu Y, Park K, Oh H, Lee S, Park S, Lee W, Cho K. Anti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract. Research In Veterinary Science 2014, 96: 567-575. PMID: 24704033, DOI: 10.1016/j.rvsc.2014.03.011.Peer-Reviewed Original ResearchConceptsCombination therapyAnti-rotaviral activityAnti-rotavirus effectIntestinal lesion scoresLethal dose 50Rotavirus enteritisCurative medicationsRotaviral enteritisSophora flavescensRotaviral diarrheaOrgan weightsPiglet modelPathological changesFecal virusBody weightTherapyVirus replicationLesion scoresSingle treatmentDose 50Porcine rotavirusAdverse effectsPreliminary dataDiarrheaEnteritis
2013
Molecular epidemiology of Korean porcine sapeloviruses
Son K, Kim D, Matthijnssens J, Kwon H, Park J, Hosmillo M, Alfajaro M, Ryu E, Kim J, Kang M, Cho K. Molecular epidemiology of Korean porcine sapeloviruses. Archives Of Virology 2013, 159: 1175-1180. PMID: 24232913, PMCID: PMC7087272, DOI: 10.1007/s00705-013-1901-6.Peer-Reviewed Original ResearchConceptsGenetic diversityPorcine sapelovirusPronounced genetic diversityVP1 geneDiarrhea fecal samplesPhylogenetic analysisPrimer pairsPSV infectionGenesPCR assaysDiversityRT-PCRKorean pigsSapelovirusFecal samplesMolecular epidemiologyDiarrhea samplesBacteriaInfectionEnteric virusesHigher proportionAssaysPigsStrains
2012
Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea
Alfajaro M, Kim H, Park J, Ryu E, Kim J, Jeong Y, Kim D, Hosmillo M, Son K, Lee J, Kwon H, Ryu Y, Park S, Park S, Lee W, Cho K. Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea. Virology Journal 2012, 9: 310. PMID: 23244491, PMCID: PMC3547719, DOI: 10.1186/1743-422x-9-310.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAnti-Inflammatory Agents, Non-SteroidalAntiviral AgentsCell LineColostrumDiarrheaDose-Response Relationship, DrugDrug Evaluation, PreclinicalFecesGlycyrrhiza uralensisInterleukin-8Intestine, SmallMAP Kinase Signaling SystemModels, AnimalNF-kappa BPhytotherapyPlant ExtractsPlant RootsRNA, MessengerRotavirusRotavirus InfectionsSpleenSwineSwine DiseasesTumor Necrosis Factor-alphaVirus SheddingConceptsInflammation-related cytokinesGlycyrrhiza uralensis extractMRNA expression levelsSmall intestineRotavirus diarrheaRotaviral enteritisAnti-rotaviral effectAnti-inflammatory effectsFecal virus sheddingOnset of diarrheaColostrum-deprived pigletsExpression levelsIntestinal lesion scoresFecal consistency scoreAntiviral efficacyVirus sheddingHistological changesSevere gastroenteritisFecal virusAntiviral agentsDiarrheaLesion scoresViable medicationsHerbal medicineAntiviral propertiesPathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets
Kim H, Park J, Alfajaro M, Kim D, Hosmillo M, Son K, Lee J, Bae Y, Park S, Kang M, Cho K. Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets. Veterinary Microbiology 2012, 159: 11-22. PMID: 22465801, DOI: 10.1016/j.vetmic.2012.03.017.Peer-Reviewed Original ResearchConceptsExtra-intestinal organsMesenteric lymph nodesCross-species pathogenicityReassortant rotavirusesLymph nodesIntestinal pathologyPersistent severe diarrheaSevere intestinal pathologyExtra-intestinal infectionsDiarrhea fecal samplesReal-time RT-PCRGreen real-time RT-PCRSYBR Green real-time RT-PCRSevere diarrheaCerebrospinal fluidChoroid plexusViral replicationRotavirusRT-PCRViral RNAFecal samplesPigletsDiarrheaPublic healthPathology
2011
Reassortment among bovine, porcine and human rotavirus strains results in G8P[7] and G6P[7] strains isolated from cattle in South Korea
Park S, Matthijnssens J, Saif L, Kim H, Park J, Alfajaro M, Kim D, Son K, Yang D, Hyun B, Kang M, Cho K. Reassortment among bovine, porcine and human rotavirus strains results in G8P[7] and G6P[7] strains isolated from cattle in South Korea. Veterinary Microbiology 2011, 152: 55-66. PMID: 21592683, DOI: 10.1016/j.vetmic.2011.04.015.Peer-Reviewed Original ResearchConceptsGenotype constellationT1-E1-H1 genotypesH1 genotype constellationFull-length ORFDetailed phylogenetic analysisGroup A rotavirusesC1-M2Phylogenetic analysisComplete genomeN2-T6Genome constellationM2-A3VP1-4I5-R1Bovine group A rotavirusesHeterologous speciesI2-R2A1-N1ReassortmentG8 genotypeSingle strainReassortant strainsGenotypesStrainsGenome
2010
Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
Hosmillo M, Jeong Y, Kim H, Collantes T, Alfajaro M, Park J, Kim H, Kwon H, Park S, Kang M, Park S, Cho K. Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses. Journal Of Virological Methods 2010, 168: 212-217. PMID: 20558206, PMCID: PMC7112831, DOI: 10.1016/j.jviromet.2010.06.001.Peer-Reviewed Original Research