2012
Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets
Kim H, Park J, Alfajaro M, Kim D, Hosmillo M, Son K, Lee J, Bae Y, Park S, Kang M, Cho K. Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets. Veterinary Microbiology 2012, 159: 11-22. PMID: 22465801, DOI: 10.1016/j.vetmic.2012.03.017.Peer-Reviewed Original ResearchConceptsExtra-intestinal organsMesenteric lymph nodesCross-species pathogenicityReassortant rotavirusesLymph nodesIntestinal pathologyPersistent severe diarrheaSevere intestinal pathologyExtra-intestinal infectionsDiarrhea fecal samplesReal-time RT-PCRGreen real-time RT-PCRSYBR Green real-time RT-PCRSevere diarrheaCerebrospinal fluidChoroid plexusViral replicationRotavirusRT-PCRViral RNAFecal samplesPigletsDiarrheaPublic healthPathology
2011
Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets
Kim H, Park J, Matthijnssens J, Lee J, Bae Y, Alfajaro M, Park S, Kang M, Cho K. Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. Veterinary Microbiology 2011, 152: 291-303. PMID: 21658866, DOI: 10.1016/j.vetmic.2011.05.017.Peer-Reviewed Original ResearchConceptsGroup A rotavirusesExtra-intestinal spreadCross-species pathogenicityPost-inoculation day 1Intestinal villous atrophyVirus-inoculated calvesExtra-intestinal organsMesenteric lymph nodesBovine group A rotavirusesColostrum-deprived calvesViral RNAReal-time RT-PCRNSP4 gene segmentsGreen real-time RT-PCRSYBR Green real-time RT-PCRVillous atrophyLymph nodesClinical symptomsGene segmentsSevere diarrheaCerebrospinal fluidChoroid plexusDay 1Viral replicationReassortant rotaviruses
2010
Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
Hosmillo M, Jeong Y, Kim H, Collantes T, Alfajaro M, Park J, Kim H, Kwon H, Park S, Kang M, Park S, Cho K. Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses. Journal Of Virological Methods 2010, 168: 212-217. PMID: 20558206, PMCID: PMC7112831, DOI: 10.1016/j.jviromet.2010.06.001.Peer-Reviewed Original ResearchConceptsGreen real-time RT-PCRSYBR Green real-time RT-PCRCopies/reactionReal-time RT-PCRRapid detectionRT-PCRMolecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows
Hosmillo M, Jeong Y, Kim H, Park J, Nayak M, Alfajaro M, Collantes T, Park S, Ikuta K, Yunoki M, Kang M, Park S, Cho K. Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows. Archives Of Virology 2010, 155: 1157-1161. PMID: 20458510, DOI: 10.1007/s00705-010-0679-z.Peer-Reviewed Original ResearchConceptsPorcine circovirus type 2Polymerase chain reactionHepatitis E virus RNAGenotype 3 HEVHEV-positive samplesHepatitis E virusReal-time RT-PCRViral copies/HEV infectionHEV RNATransplacental infectionCircovirus type 2E virusCopies/Type 2PCV2 coinfectionSerum samplesFetusesRT-PCRVirus RNAChain reactionReproductive failureInfectionReverse transcriptionMolecular detection