Phosphatidylinositol 3-Kinase/Akt and MEK/ERK Signaling Pathways Facilitate Sapovirus Trafficking and Late Endosomal Acidification for Viral Uncoating in LLC-PK Cells
Soliman M, Kim D, Park J, Kim J, Alfajaro M, Baek Y, Cho E, Park C, Kang M, Park S, Cho K. Phosphatidylinositol 3-Kinase/Akt and MEK/ERK Signaling Pathways Facilitate Sapovirus Trafficking and Late Endosomal Acidification for Viral Uncoating in LLC-PK Cells. Journal Of Virology 2018, 92: 10.1128/jvi.01674-18. PMID: 30282712, PMCID: PMC6258943, DOI: 10.1128/jvi.01674-18.Peer-Reviewed Original ResearchConceptsMEK/ERKCell surface carbohydrate receptorsLate endosomesPI3K/AktSmall interfering RNAsEndosomal acidificationPI3KEarly endosomesExtracellular signal-regulated kinaseViral uncoatingSignal-regulated kinaseV-ATPase proton pumpCell surface receptorsHost cell entryEarly activationEntry processERK moleculesInterfering RNAsEndosomesCarbohydrate receptorsUse of inhibitorsProton pumpERKSurface receptorsAktRotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells
Soliman M, Cho E, Park J, Kim J, Alfajaro M, Baek Y, Kim D, Kang M, Park S, Cho K. Rotavirus-Induced Early Activation of the RhoA/ROCK/MLC Signaling Pathway Mediates the Disruption of Tight Junctions in Polarized MDCK Cells. Scientific Reports 2018, 8: 13931. PMID: 30224682, PMCID: PMC6141481, DOI: 10.1038/s41598-018-32352-y.Peer-Reviewed Original ResearchConceptsTJ protein distributionTJ integrityTJ proteinsTight junctionsIntestinal epithelial tight junctionsEarly disruptionMDCK cellsEpithelial tight junctionsPrecise molecular mechanismsBovine NCDVRotavirus strainsEarly activationParacellular permeabilityCellular receptorsPerijunctional actomyosin ringTransepithelial resistanceEntry portalReversible decreaseSignaling pathwaysInfectionCoreceptorHarmful factorsMolecular mechanismsPresent studyCells