2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2001
The Yeast Class V Myosins, Myo2p and Myo4p, Are Nonprocessive Actin-Based Motors
Reck-Peterson S, Tyska M, Novick P, Mooseker M. The Yeast Class V Myosins, Myo2p and Myo4p, Are Nonprocessive Actin-Based Motors. Journal Of Cell Biology 2001, 153: 1121-1126. PMID: 11381095, PMCID: PMC2174330, DOI: 10.1083/jcb.153.5.1121.Peer-Reviewed Original ResearchActinsAdenosine TriphosphateAnimalsAntibodiesBrainCalciumCalmodulin-Binding ProteinsCarrier ProteinsChickensFungal ProteinsKineticsMicroscopy, VideoMolecular Motor ProteinsMovementMyosin Heavy ChainsMyosin Type IIMyosin Type VMyosinsNerve Tissue ProteinsProtein BindingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe Proteins
2000
Myosin-V stepping kinetics: A molecular model for processivity
Rief M, Rock R, Mehta A, Mooseker M, Cheney R, Spudich J. Myosin-V stepping kinetics: A molecular model for processivity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 9482-9486. PMID: 10944217, PMCID: PMC16890, DOI: 10.1073/pnas.97.17.9482.Peer-Reviewed Original Research
1999
The Tail of a Yeast Class V Myosin, Myo2p, Functions as a Localization Domain
Reck-Peterson S, Novick P, Mooseker M. The Tail of a Yeast Class V Myosin, Myo2p, Functions as a Localization Domain. Molecular Biology Of The Cell 1999, 10: 1001-1017. PMID: 10198053, PMCID: PMC25227, DOI: 10.1091/mbc.10.4.1001.Peer-Reviewed Original ResearchActinsCarrier ProteinsCell FractionationCell PolarityFungal ProteinsKineticsMutagenesis, Site-DirectedMyosin Heavy ChainsMyosin Type IIMyosin Type VMyosinsPhenotypePolymerase Chain ReactionRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsSubcellular Fractions
1996
Enzymatic Characterization and Functional Domain Mapping of Brain Myosin-V*
Nascimento A, Cheney R, Tauhata S, Larson R, Mooseker M. Enzymatic Characterization and Functional Domain Mapping of Brain Myosin-V*. Journal Of Biological Chemistry 1996, 271: 17561-17569. PMID: 8663447, DOI: 10.1074/jbc.271.29.17561.Peer-Reviewed Original Research
1993
Calcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry
Wolenski J, Hayden S, Forscher P, Mooseker. Calcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry. Journal Of Cell Biology 1993, 122: 613-621. PMID: 8335688, PMCID: PMC2119657, DOI: 10.1083/jcb.122.3.613.Peer-Reviewed Original Research
1990
Binding of brush border myosin I to phospholipid vesicles.
Hayden S, Wolenski J, Mooseker M. Binding of brush border myosin I to phospholipid vesicles. Journal Of Cell Biology 1990, 111: 443-451. PMID: 2143194, PMCID: PMC2116197, DOI: 10.1083/jcb.111.2.443.Peer-Reviewed Original ResearchConceptsBB myosin IMyosin IBrush border myosin IMyosin I heavy chainMembrane-binding domainActin filament corePhospholipid vesiclesATP-sensitive mannerPlasma membraneIntestinal epithelial cellsF-actinMembrane interactionsPhosphatidylglycerol vesiclesFree proteinCOOH-terminalAnionic phospholipidsImmunoblot analysisVesiclesEpithelial cellsImmunoblot stainingM. SimilarHeavy chainActinStructural informationNeutral phospholipids
1989
Phosphorylation of the tight-junction protein ZO-1 in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance
Stevenson B, Anderson J, Braun I, Mooseker M. Phosphorylation of the tight-junction protein ZO-1 in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance. Biochemical Journal 1989, 263: 597-599. PMID: 2597123, PMCID: PMC1133468, DOI: 10.1042/bj2630597.Peer-Reviewed Original Research
1988
Characterization of villin from the intestinal brush border of the rat, and comparative analysis with avian villin
Alicea H, Mooseker M. Characterization of villin from the intestinal brush border of the rat, and comparative analysis with avian villin. Cytoskeleton 1988, 9: 60-72. PMID: 3356045, DOI: 10.1002/cm.970090107.Peer-Reviewed Original Research
1987
Erythrocyte adducin: a calmodulin-regulated actin-bundling protein that stimulates spectrin-actin binding.
Mische S, Mooseker M, Morrow J. Erythrocyte adducin: a calmodulin-regulated actin-bundling protein that stimulates spectrin-actin binding. Journal Of Cell Biology 1987, 105: 2837-2845. PMID: 3693401, PMCID: PMC2114693, DOI: 10.1083/jcb.105.6.2837.Peer-Reviewed Original ResearchNucleation of actin polymerization by villin and elongation at subcritical monomer concentration.
Weber A, Northrop J, Bishop M, Ferrone F, Mooseker M. Nucleation of actin polymerization by villin and elongation at subcritical monomer concentration. Biochemistry 1987, 26: 2528-36. PMID: 3607030, DOI: 10.1021/bi00383a019.Peer-Reviewed Original ResearchKinetics of actin elongation and depolymerization at the pointed end.
Weber A, Northrop J, Bishop M, Ferrone F, Mooseker M. Kinetics of actin elongation and depolymerization at the pointed end. Biochemistry 1987, 26: 2537-44. PMID: 3607031, DOI: 10.1021/bi00383a020.Peer-Reviewed Original Research
1986
Different calcium dependence of the capping and cutting activities of villin.
Northrop J, Weber A, Mooseker M, Franzini-Armstrong C, Bishop M, Dubyak G, Tucker M, Walsh T. Different calcium dependence of the capping and cutting activities of villin. Journal Of Biological Chemistry 1986, 261: 9274-9281. PMID: 3087992, DOI: 10.1016/s0021-9258(18)67650-1.Peer-Reviewed Original ResearchCytochalasin B slows but does not prevent monomer addition at the barbed end of the actin filament.
Bonder E, Mooseker M. Cytochalasin B slows but does not prevent monomer addition at the barbed end of the actin filament. Journal Of Cell Biology 1986, 102: 282-288. PMID: 3941155, PMCID: PMC2114038, DOI: 10.1083/jcb.102.1.282.Peer-Reviewed Original Research
1985
Effects of actin filament cross-linking and filament length on actin-myosin interaction.
Coleman T, Mooseker M. Effects of actin filament cross-linking and filament length on actin-myosin interaction. Journal Of Cell Biology 1985, 101: 1850-1857. PMID: 2932451, PMCID: PMC2113970, DOI: 10.1083/jcb.101.5.1850.Peer-Reviewed Original Research
1984
Calcium dependence of villin-induced actin depolymerization.
Walsh T, Weber A, Davis K, Bonder E, Mooseker M. Calcium dependence of villin-induced actin depolymerization. Biochemistry 1984, 23: 6099-102. PMID: 6525347, DOI: 10.1021/bi00320a030.Peer-Reviewed Original ResearchEffect of villin on the kinetics of actin polymerization.
Walsh T, Higgins J, Weber A, Bonder E, Mooseker M. Effect of villin on the kinetics of actin polymerization. Biochemistry 1984, 23: 2613-21. PMID: 6432033, DOI: 10.1021/bi00307a012.Peer-Reviewed Original Research
1983
Effects of villin on the polymerization and subunit exchange of actin
Wang Y, Bonder E, Mooseker M, Taylor D. Effects of villin on the polymerization and subunit exchange of actin. Cytoskeleton 1983, 3: 151-165. PMID: 6883468, DOI: 10.1002/cm.970030205.Peer-Reviewed Original Research
1982
Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro.
Keller T, Mooseker M. Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro. Journal Of Cell Biology 1982, 95: 943-959. PMID: 6897550, PMCID: PMC2112925, DOI: 10.1083/jcb.95.3.943.Peer-Reviewed Original ResearchConceptsBrush border contractionBrush borderIntestinal epithelial cellsEpithelial cellsCalmodulin activityBrush border proteinsMyosin light chain kinaseContractionDegrees CLight chain kinaseLight chainCalmodulin-dependent phosphorylationBrush border myosinPhosphorylationDalton light chainChain kinaseTerminal web