2007
Roles for Drosophila melanogaster Myosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial Pathogen Pseudomonas entomophila
Hegan PS, Mermall V, Tilney LG, Mooseker MS. Roles for Drosophila melanogaster Myosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial Pathogen Pseudomonas entomophila. Molecular Biology Of The Cell 2007, 18: 4625-4636. PMID: 17855510, PMCID: PMC2043548, DOI: 10.1091/mbc.e07-02-0191.Peer-Reviewed Original ResearchConceptsMutant larvaePseudomonas entomophilaTail domainMyosin IBMidgut epithelial cellsActin filament coreDrosophila genomeApical brush borderMidgut enterocytesBrush borderCytoskeletal defectsLarval gutResponse pathwaysApical localizationMV membraneBrush border structureDeletion mutationsFood uptakeGram-negative bacterial infectionsInnate immune responseMyo1bGut physiologyEntomophilaEpithelial cellsMidgut
1997
An actin-related protein in Drosophila colocalizes with heterochromatin protein 1 in pericentric heterochromatin
Frankel S, Sigel E, Craig C, Elgin S, Mooseker M, Artavanis-Tsakonas S. An actin-related protein in Drosophila colocalizes with heterochromatin protein 1 in pericentric heterochromatin. Journal Of Cell Science 1997, 110: 1999-2012. PMID: 9378752, DOI: 10.1242/jcs.110.17.1999.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAntibodiesBacterial ProteinsCarrier ProteinsCell NucleusChromatinChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneChromosomesDrosophila melanogasterEmbryo, NonmammalianEuchromatinGene Expression Regulation, DevelopmentalGenes, InsectHeterochromatinMutagenesisNuclear ProteinsRatsConceptsHeterochromatin protein 1Actin-related proteinsPolytene chromosome spreadsLocalization patternsCentric heterochromatinChromosome spreadsEuchromatic bandsProtein 1Heterochromatin-mediated geneNuclear localization patternPericentric heterochromatinHeterochromatin functionChromatin structureNuclear functionsFunctional diversitySequence similarityPolytene nucleiArp4Larval tissuesPrepupal developmentGenetic evidenceMutant formsGenetic dataHeterochromatinCell cycle
1995
Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton
Morgan N, Heintzelman M, Mooseker M. Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton. Developmental Biology 1995, 172: 51-71. PMID: 7589814, DOI: 10.1006/dbio.1995.0005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCloning, MolecularCytoskeletonDrosophila melanogasterElectrophoresis, Polyacrylamide GelEmbryo, NonmammalianFemaleGene Expression RegulationImmunoblottingImmunohistochemistryLarvaMicroscopy, ElectronMicroscopy, ImmunoelectronMicrovilliMyosinsOogenesisOvumPupaRecombinant ProteinsConceptsMyosin IBAdult gutATP-dependent extractionMyosin IASomatic follicle cellsExogenous F-actinBrush border cytoskeletonNovel unconventional myosinDrosophila melanogasterMicrovillus assemblyEgg chambersApical domainMicrovillar componentsMicrovillar domainApical microvillar domainBasolateral domainExpression patternsUnconventional myosinF-actinSubcellular distributionFollicle cellsFunctional myosinDifferentiated enterocytesBiochemical propertiesBrush border
1994
The Molecular Cloning and Characterization of Drosophila melanogaster Myosin-IA and Myosin-IB
Morgan N, Skovronsky D, Artavanis-Tsakonas S, Mooseker M. The Molecular Cloning and Characterization of Drosophila melanogaster Myosin-IA and Myosin-IB. Journal Of Molecular Biology 1994, 239: 347-356. PMID: 8201616, DOI: 10.1006/jmbi.1994.1376.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCloning, MolecularConsensus SequenceConserved SequenceDrosophila melanogasterGenes, InsectLarvaMolecular Sequence DataMyosinsProtein Structure, TertiaryRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTranscription, GeneticConceptsDeduced amino acid sequenceKilobase cDNA cloneAmino acid sequenceMyosin IBCDNA clonesIQ motifGene productsAcid sequenceMyosin I familyDrosophila cDNA libraryPrimary sequence analysisMyosin IANorthern blot analysisVertebrate homologsEmbryonic libraryDrosophila melanogasterMolecular cloningSequence comparisonNovel myosinCDNA libraryTail domainHead libraryUnique tailDegenerate primersUnconventional myosin
1993
Cloning of a Secretory Gelsolin from Drosophila melanogaster
Heintzelman M, Frankel S, Artavanis-Tsakonas S, Mooseker M. Cloning of a Secretory Gelsolin from Drosophila melanogaster. Journal Of Molecular Biology 1993, 230: 709-716. PMID: 8386771, DOI: 10.1006/jmbi.1993.1191.Peer-Reviewed Original ResearchConceptsSecretory gelsolinAcid sequenceThird instar Drosophila larvaeDeduced amino acid sequenceFull-length complementary DNAInstar Drosophila larvaeClass of proteinsActin-binding proteinsAmino acid sequenceAmino acid residuesGelsolin sequenceThird chromosomeDrosophila melanogasterGelsolin familyCytoplasmic proteinsSignal peptideSingle geneDrosophila larvaeRepeat structureActin filamentsMonomeric actinAcid residuesBarbed endsPrimary structureNorthern blot