2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
2001
Myosin-VIIb, a Novel Unconventional Myosin, Is a Constituent of Microvilli in Transporting Epithelia
Chen Z, Hasson T, Zhang D, Schwender B, Derfler B, Mooseker M, Corey D. Myosin-VIIb, a Novel Unconventional Myosin, Is a Constituent of Microvilli in Transporting Epithelia. Genomics 2001, 72: 285-296. PMID: 11401444, DOI: 10.1006/geno.2000.6456.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsBase SequenceBiological TransportBlotting, NorthernBlotting, WesternChromosome MappingCloning, MolecularDNA, ComplementaryEpitheliumExonsFemaleGenesImmunohistochemistryIntestinesIntronsKidneyMiceMice, Inbred BALB CMicrovilliMolecular Sequence DataMyosinsPhylogenyProtein IsoformsRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTissue Distribution
1999
Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine
Skowron J, Mooseker M. Cloning and characterization of mouse brush border myosin‐I in adult and embryonic intestine. Journal Of Experimental Zoology 1999, 283: 242-257. PMID: 9933937, DOI: 10.1002/(sici)1097-010x(19990215)283:3<242::aid-jez3>3.0.co;2-f.Peer-Reviewed Original ResearchConceptsBrush border myosinMicrovillar actin bundlesEmbryonic intestineSubcellular localization profileBasolateral membrane domainsGenomic clonesCalmodulin light chainsVertebrate speciesMembrane domainsActin corePlasma membraneKnockout strategiesActin bundlesImmunolocalization studiesIntestinal epithelial cellsGolgi apparatusNorthern analysisPrimary structureNorthern blotSitu localizationLocalization profilesRNA expressionEmbryosDirect targetingEmbryogenesis
1998
Human brush border myosin‐I and myosin‐Ic expression in human intestine and Caco‐2BBe cells
Skowron J, Bement W, Mooseker M. Human brush border myosin‐I and myosin‐Ic expression in human intestine and Caco‐2BBe cells. Cytoskeleton 1998, 41: 308-324. PMID: 9858156, DOI: 10.1002/(sici)1097-0169(1998)41:4<308::aid-cm4>3.0.co;2-j.Peer-Reviewed Original Research
1996
Molecular Cloning and Domain Structure of Human Myosin-VIIa, the Gene Product Defective in Usher Syndrome 1B
Chen Z, Hasson T, Kelley P, Schwender B, Schwartz M, Ramakrishnan M, Kimberling W, Mooseker M, Corey D. Molecular Cloning and Domain Structure of Human Myosin-VIIa, the Gene Product Defective in Usher Syndrome 1B. Genomics 1996, 36: 440-448. PMID: 8884267, DOI: 10.1006/geno.1996.0489.Peer-Reviewed Original ResearchConceptsUsher syndrome 1BIntestinal epithelium cell lineProgressive retinal degenerationEpithelium cell lineVestibular functionMyosin VIIaPigment epitheliumRetinal degenerationInner earReceptor cellsRT-PCRBand 4.1 familyRestricted expressionShort formCell linesMembrane-associated portionHuman myosin VIIaMolecular cloningLong transcriptsTestisShort transcriptsUnconventional myosinLong repeatsHigh abundanceAmino acids
1995
The mouse Snell's waltzer deafness gene encodes an unconventional myosin required for structural integrity of inner ear hair cells
Avraham K, Hasson T, Steel K, Kingsley D, Russell L, Mooseker M, Copeland N, Jenkins N. The mouse Snell's waltzer deafness gene encodes an unconventional myosin required for structural integrity of inner ear hair cells. Nature Genetics 1995, 11: 369-375. PMID: 7493015, DOI: 10.1038/ng1295-369.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceChromosome InversionCloning, MolecularDeafnessDNA Mutational AnalysisGenes, RecessiveHair Cells, Auditory, InnerHumansMiceMice, Inbred C57BLMice, Mutant StrainsMolecular Sequence DataMyosin Heavy ChainsOrgan of CortiRestriction MappingRNA, MessengerSequence DeletionConceptsMyosin VIUnconventional myosin heavy chainPositional cloning approachInner ear hair cellsHuman deafness disordersExcellent model systemEar hair cellsSensory hair cellsHair cellsDeafness disordersCloning approachUnconventional myosinDeafness mutationsDeafness mutantsDeafness genesMyosin heavy chainGenesGenetic deafnessModel systemHeavy chainStructural integrityWaltzerInner earCellsMutantsCharacterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton
Morgan N, Heintzelman M, Mooseker M. Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton. Developmental Biology 1995, 172: 51-71. PMID: 7589814, DOI: 10.1006/dbio.1995.0005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCloning, MolecularCytoskeletonDrosophila melanogasterElectrophoresis, Polyacrylamide GelEmbryo, NonmammalianFemaleGene Expression RegulationImmunoblottingImmunohistochemistryLarvaMicroscopy, ElectronMicroscopy, ImmunoelectronMicrovilliMyosinsOogenesisOvumPupaRecombinant ProteinsConceptsMyosin IBAdult gutATP-dependent extractionMyosin IASomatic follicle cellsExogenous F-actinBrush border cytoskeletonNovel unconventional myosinDrosophila melanogasterMicrovillus assemblyEgg chambersApical domainMicrovillar componentsMicrovillar domainApical microvillar domainBasolateral domainExpression patternsUnconventional myosinF-actinSubcellular distributionFollicle cellsFunctional myosinDifferentiated enterocytesBiochemical propertiesBrush border
1994
Porcine myosin-VI: characterization of a new mammalian unconventional myosin.
Hasson T, Mooseker M. Porcine myosin-VI: characterization of a new mammalian unconventional myosin. Journal Of Cell Biology 1994, 127: 425-440. PMID: 7929586, PMCID: PMC2120210, DOI: 10.1083/jcb.127.2.425.Peer-Reviewed Original ResearchCloning and mRNA Expression of Human Unconventional Myosin-IC A Homologue of Amoeboid Myosins-I With a Single IQ Motif and an SH3 Domain
Bement W, Wirth J, Mooseker M. Cloning and mRNA Expression of Human Unconventional Myosin-IC A Homologue of Amoeboid Myosins-I With a Single IQ Motif and an SH3 Domain. Journal Of Molecular Biology 1994, 243: 356-363. PMID: 7932763, DOI: 10.1006/jmbi.1994.1662.Peer-Reviewed Original ResearchConceptsSrc homology 3 domainSingle IQ motifIQ motifComplete deduced amino acid sequenceDeduced amino acid sequenceSingle open reading frameC-terminal tailOpen reading frameATP-binding siteAmino acid sequenceMembrane-binding sitesMultiple sequence alignmentNorthern blot analysisSH3 domainCDNA clonesReading frameAcid sequenceSequence alignmentMyosin ICThe Molecular Cloning and Characterization of Drosophila melanogaster Myosin-IA and Myosin-IB
Morgan N, Skovronsky D, Artavanis-Tsakonas S, Mooseker M. The Molecular Cloning and Characterization of Drosophila melanogaster Myosin-IA and Myosin-IB. Journal Of Molecular Biology 1994, 239: 347-356. PMID: 8201616, DOI: 10.1006/jmbi.1994.1376.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCloning, MolecularConsensus SequenceConserved SequenceDrosophila melanogasterGenes, InsectLarvaMolecular Sequence DataMyosinsProtein Structure, TertiaryRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTranscription, GeneticConceptsDeduced amino acid sequenceKilobase cDNA cloneAmino acid sequenceMyosin IBCDNA clonesIQ motifGene productsAcid sequenceMyosin I familyDrosophila cDNA libraryPrimary sequence analysisMyosin IANorthern blot analysisVertebrate homologsEmbryonic libraryDrosophila melanogasterMolecular cloningSequence comparisonNovel myosinCDNA libraryTail domainHead libraryUnique tailDegenerate primersUnconventional myosin
1993
Cloning of a Secretory Gelsolin from Drosophila melanogaster
Heintzelman M, Frankel S, Artavanis-Tsakonas S, Mooseker M. Cloning of a Secretory Gelsolin from Drosophila melanogaster. Journal Of Molecular Biology 1993, 230: 709-716. PMID: 8386771, DOI: 10.1006/jmbi.1993.1191.Peer-Reviewed Original ResearchConceptsSecretory gelsolinAcid sequenceThird instar Drosophila larvaeDeduced amino acid sequenceFull-length complementary DNAInstar Drosophila larvaeClass of proteinsActin-binding proteinsAmino acid sequenceAmino acid residuesGelsolin sequenceThird chromosomeDrosophila melanogasterGelsolin familyCytoplasmic proteinsSignal peptideSingle geneDrosophila larvaeRepeat structureActin filamentsMonomeric actinAcid residuesBarbed endsPrimary structureNorthern blot
1989
Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family.
Garcia A, Coudrier E, Carboni J, Anderson J, Vandekerkhove J, Mooseker M, Louvard D, Arpin M. Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family. Journal Of Cell Biology 1989, 109: 2895-2903. PMID: 2687288, PMCID: PMC2115973, DOI: 10.1083/jcb.109.6.2895.Peer-Reviewed Original Research