1995
Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor‐α, and insulinlike growth factor‐II on Na+/H+ exchange activity
Strazzabosco M, Poci C, Spirlì C, Zsembery A, Granato A, Massimino M, Crepaldi G. Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor‐α, and insulinlike growth factor‐II on Na+/H+ exchange activity. Hepatology 1995, 22: 588-597. PMID: 7635429, DOI: 10.1002/hep.1840220232.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmilorideAmmonium ChlorideAnimalsCarcinoma, HepatocellularCarrier ProteinsCattleCell DivisionDNAEpidermal Growth FactorFetal BloodFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationInsulin-Like Growth Factor IILiver NeoplasmsSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersTransforming Growth Factor alphaTumor Cells, CulturedConceptsInsulinlike growth factor IIEpidermal growth factorTGF-alphaGrowth factor IIHep G2 cellsGrowth factorFactor IIG2 cellsAdministration of EGFLiver growth factorCl/HCO3 exchangeExtrahepatic tumorsAcid/base homeostasisHepatic tumorsAcid loaderFetal calf serumAcid extrudersHepatoma cell linePresence of FCSAcid loadExchange activityMetabolic activationHCO3- exchangeProliferative stimulusAmilorideIntracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor-α, and insulinlike growth factor-II on Na+H+ exchange activity
Strazzabosco M, Poci C, Spirlì C, Zsembery A, Granato A, Massimino M, Crepaldi G. Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor-α, and insulinlike growth factor-II on Na+H+ exchange activity. Hepatology 1995, 22: 588-597. DOI: 10.1016/0270-9139(95)90584-7.Peer-Reviewed Original ResearchInsulinlike growth factor IIEpidermal growth factorGrowth factor IIHep G2 cellsGrowth factorFactor IIG2 cellsAdministration of EGFLiver growth factorCl/HCO3 exchangeExtrahepatic tumorsAcid/base homeostasisHepatic tumorsAcid loaderFetal calf serumAcid extrudersHepatoma cell linePresence of FCSAcid loadExchange activityMetabolic activationHCO3- exchangeProliferative stimulusTGFαAmiloride
1994
Effect of ursodeoxycholic acid on intracellular pH in a bile duct epithelium‐like cell line
Strazzabosco M, Poci C, Spirlí C, Sartori L, Knuth A, Crepaldi G. Effect of ursodeoxycholic acid on intracellular pH in a bile duct epithelium‐like cell line. Hepatology 1994, 19: 145-154. PMID: 8276351, DOI: 10.1002/hep.1840190124.Peer-Reviewed Original ResearchConceptsUrsodeoxycholic acidDuct epitheliumUnconjugated ursodeoxycholic acidSK-ChA-1 cellsBile duct epitheliumSK-ChA-1Acid-induced increaseFluorescent indicator 2Human cholangiocarcinoma cell linesCell linesIntracellular pH regulatory mechanismsCholangiocarcinoma cell linesCl/HCO3 exchangePreincubation of cellsAbsence of HCO3Furosemide administrationPH regulatory mechanismsAcid/base transportersDuct levelUDCAHCO3- exchange
1991
Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers
Strazzabosco M, Sakisaka S, Hayakawa T, Boyer J. Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers. American Journal Of Physiology 1991, 260: g58-g69. PMID: 1987808, DOI: 10.1152/ajpgi.1991.260.1.g58.Peer-Reviewed Original ResearchConceptsBiliary HCO3- concentrationUrsodeoxycholic acidRat hepatocyte coupletsBiliary pHAcid loadEffect of UDCABile duct lumenHepatocyte coupletsIsolated rat hepatocyte coupletsBiliary epitheliumHCO3- concentrationDuct lumenRemoval of ClHCO3- exchangeRat liverLiverIPRLHepatocytesHypercholeresisPerfusateBileExcretionEpitheliumAmiloride