2022
Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation
Habertheuer A, Chatterjee S, Sada Japp A, Ram C, Korutla L, Ochiya T, Li W, Terada Y, Takahashi T, Nava RG, Puri V, Kreisel D, Vallabhajosyula P. Donor extracellular vesicle trafficking via the pleural space represents a novel pathway for allorecognition after lung transplantation. American Journal Of Transplantation 2022, 22: 1909-1918. PMID: 35285127, DOI: 10.1111/ajt.17023.Peer-Reviewed Original ResearchConceptsMediastinal lymph nodesLymph nodesLung transplantationDonor antigensPleural spaceBronchial anastomosisGraft-draining lymph nodesLocoregional lymph nodesLung transplant recipientsPeripheral lymph nodesTransplant recipientsAllorecognition pathwaysPulmonary transplantationMHC-IIPleural fluidRat modelT cellsLymphatic drainageTransplantationDonor extracellular vesiclesPleural lymphaticsCell traffickingRapid rejectionAlternative pathwayAntigen
2021
Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model
Habertheuer A, Ram C, Schmierer M, Chatterjee S, Hu R, Freas A, Zielinski P, Rogers W, Silvestro EM, McGrane M, Moore JS, Korutla L, Siddiqui S, Xin Y, Rizi R, Tao J, Kreisel D, Naji A, Ochiya T, Vallabhajosyula P. Circulating Donor Lung-specific Exosome Profiles Enable Noninvasive Monitoring of Acute Rejection in a Rodent Orthotopic Lung Transplantation Model. Transplantation 2021, 106: 754-766. PMID: 33993180, DOI: 10.1097/tp.0000000000003820.Peer-Reviewed Original ResearchConceptsAcute rejectionDonor exosomesExosome profileSyngeneic controlsRat orthotopic lung transplant modelChronic lung allograft dysfunctionOrthotopic lung transplantation modelLung transplant modelPosttransplant time pointsAcute rejection episodesLung allograft dysfunctionLung transplant patientsLung transplant rejectionLung transplantation modelEarly therapeutic interventionMajor risk factorTime-sensitive diagnosisDevelopment of biomarkersAllograft dysfunctionRejection episodesLewis recipientsTransplant patientsPulmonary allograftsRecipient bloodAllogeneic grafts
2012
Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis
Scofield M, Korutla L, Jackson T, Kalivas P, Mackler S. Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis. Neuroscience 2012, 227: 44-54. PMID: 23022214, PMCID: PMC3505276, DOI: 10.1016/j.neuroscience.2012.09.043.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAspartic AcidCell DeathCholine O-AcetyltransferaseCytoplasmDNA-Binding ProteinsEmbryo, MammalianGene Expression RegulationGlial Fibrillary Acidic ProteinGlutamic AcidImmunoprecipitationNeoplasm ProteinsNeuronsPhosphopyruvate HydrataseProtein BindingRatsRepressor ProteinsSpinal CordTransfectionUbiquitinationConceptsNucleus accumbens-1Amyotrophic lateral sclerosisPOZ/BTB domainUbiquitin-proteasome systemTDP-43Protein 43Lateral sclerosisBTB domainPrimary spinal cord culturesDevelopment of ALSTAR DNA-binding protein 43POZ/BTB proteinDNA-binding protein 43Extracellular glutamate levelsTDP-43 expressionFull-length TDP-43Spinal cord culturesGST pulldown assaysPost-translational modificationsUbiquitinated protein aggregatesCholinergic neuronsGlutamate toxicityCord culturesGlutamate levelsSpinal cord
2007
NAC1 Regulates the Recruitment of the Proteasome Complex into Dendritic Spines
Shen H, Korutla L, Champtiaux N, Toda S, LaLumiere R, Vallone J, Klugmann M, Blendy J, Mackler S, Kalivas P. NAC1 Regulates the Recruitment of the Proteasome Complex into Dendritic Spines. Journal Of Neuroscience 2007, 27: 8903-8913. PMID: 17699672, PMCID: PMC6672176, DOI: 10.1523/jneurosci.1571-07.2007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBicucullineCell Cycle ProteinsCells, CulturedCerebral CortexCullin ProteinsCysteine Proteinase InhibitorsDendritic SpinesEmbryo, MammalianGABA AntagonistsGreen Fluorescent ProteinsIntracellular Signaling Peptides and ProteinsLeupeptinsMiceMice, KnockoutNerve Tissue ProteinsNeuronsProteasome Endopeptidase ComplexProtein TransportRatsRepressor ProteinsRNA-Binding ProteinsTransfectionUbiquitinConceptsUbiquitin-proteasome systemDendritic spinesPostsynaptic densityProteolysis machineryProteasome complexTranscriptional proteinsProteasomeGene deletionSynaptic proteinsProteinCortical neuronsSynaptic remodelingSynaptic activitySynaptic plasticityBicucullinePotential missing linkRecruitmentSpineNeuronsNaC1Progressive accumulationMov34CullinMachineryProteolysis
2005
The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures
Korutla L, Wang P, Mackler S. The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures. Journal Of Neurochemistry 2005, 94: 786-793. PMID: 16033423, DOI: 10.1111/j.1471-4159.2005.03206.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternButyratesCells, CulturedEnzyme InhibitorsGene Expression RegulationHippocampusHistone DeacetylasesHumansHydroxamic AcidsImmunoprecipitationMiceNerve Tissue ProteinsNeuroblastomaNeuronsPlasmidsProtein BindingProtein IsoformsRatsRepressor ProteinsTranscription, GeneticTransfectionTwo-Hybrid System TechniquesConceptsPOZ/BTB proteinProtein-protein interactionsBTB proteinsHistone deacetylasesPOZ domainTwo-hybridGlutathione S-transferase pulldownRecruit histone deacetylasesDifferent histone deacetylasesTranscriptional repressionGST pulldownSNAC1Histone deacetylase inhibitionCorepressorDeacetylasesRepressionDeacetylase inhibitionProteinPulldownHDAC-3NaC1MSin3ANucleus accumbensCNS regionsCoimmunoprecipitation
2003
The mouse nac1 gene, encoding a cocaine-regulated bric-a-brac tramtrac broad complex/pox virus and zinc finger protein, is regulated by ap1
Mackler S, Homan Y, Korutla L, Conti A, Blendy J. The mouse nac1 gene, encoding a cocaine-regulated bric-a-brac tramtrac broad complex/pox virus and zinc finger protein, is regulated by ap1. Neuroscience 2003, 121: 355-361. PMID: 14521994, DOI: 10.1016/s0306-4522(03)00376-2.Peer-Reviewed Original ResearchAnimalsBlotting, NorthernBlotting, SouthernCell DifferentiationCell Line, TumorChromosomes, Human, Pair 8Cloning, MolecularColforsinDNA ProbesDNA-Binding ProteinsDrosophilaDrosophila ProteinsExonsGene ExpressionHumansMiceMice, Inbred C57BLMutagenesisNerve Tissue ProteinsNeuroblastomaPromoter Regions, GeneticRatsRepressor ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSequence AlignmentSequence AnalysisTranscription Factor AP-1Transcription FactorsTransfectionZinc FingersNAC1, a POZ/BTB protein present in the adult mammalian brain, triggers apoptosis after adenovirus-mediated overexpression in PC-12 cells
Korutla L, Neustadter J, Fournier K, Mackler S. NAC1, a POZ/BTB protein present in the adult mammalian brain, triggers apoptosis after adenovirus-mediated overexpression in PC-12 cells. Neuroscience Research 2003, 46: 33-39. PMID: 12725910, DOI: 10.1016/s0168-0102(03)00024-5.Peer-Reviewed Original ResearchConceptsPC-12 cellsCell deathPOZ/BTB proteinPro-apoptotic protein BaxPOZ/BTBAnti-apoptotic protein Bcl-2Mammalian brainAdenoviral-mediated gene transferProtein Bcl-2BTB proteinsTranscription factorsCellular developmentGenomic DNAAdult mammalian brainNAC1 expressionProtein BaxCell survivalGene transferUpstream mediatorDown regulationNAC1 overexpressionBcl-2Examples actProteinOverexpression
2002
Differences in expression, actions and cocaine regulation of two isoforms for the brain transcriptional regulator NAC1
Korutla L, Wang P, Lewis D, Neustadter J, Stromberg M, Mackler S. Differences in expression, actions and cocaine regulation of two isoforms for the brain transcriptional regulator NAC1. Neuroscience 2002, 110: 421-429. PMID: 11906783, DOI: 10.1016/s0306-4522(01)00518-8.Peer-Reviewed Original ResearchConceptsBTB/POZ proteinPOZ proteinShort isoformDNA binding domainsPost-transcriptional processingSemi-quantitative reverse transcription-polymerase chain reaction analysisPeripheral tissuesRat brainTranscriptional repressionSubnuclear localizationTranscription-polymerase chain reaction analysisBinding domainsReverse transcription-polymerase chain reaction analysisReporter systemCell cycleCocaine-induced locomotionRat nucleus accumbensWestern blot analysisTranscriptionAmino acidsIsoformsProteinChain reaction analysisBlot analysisNeuronal function
2000
NAC-1 Is a Brain POZ/BTB Protein That Can Prevent Cocaine-Induced Sensitization in the Rat
Mackler S, Korutla L, Cha X, Koebbe M, Fournier K, Bowers M, Kalivas P. NAC-1 Is a Brain POZ/BTB Protein That Can Prevent Cocaine-Induced Sensitization in the Rat. Journal Of Neuroscience 2000, 20: 6210-6217. PMID: 10934270, PMCID: PMC6772573, DOI: 10.1523/jneurosci.20-16-06210.2000.Peer-Reviewed Original ResearchConceptsPOZ/BTB proteinBTB proteinsTranscriptional regulatorsNAC-1POZ/BTB domainZinc finger regionTwo-hybrid studiesDNA-binding proteinsBTB domainTranscription factorsGene transcriptionFinger regionReporter geneProteinNuclei of neuronsMammalian brainTranscriptionBehavioral sensitizationPox virusRegulatorCell culturesAdministration of cocaineRat nucleus accumbensExpressionLong-term neuroadaptationsActivation of surfactant protein‐B transcription: Signaling through the SP‐A receptor utilizing the PI3 kinase pathway
Strayer D, Korutla L. Activation of surfactant protein‐B transcription: Signaling through the SP‐A receptor utilizing the PI3 kinase pathway. Journal Of Cellular Physiology 2000, 184: 229-238. PMID: 10867648, DOI: 10.1002/1097-4652(200008)184:2<229::aid-jcp11>3.0.co;2-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDNA-Binding ProteinsFemaleGlycoproteinsHepatocyte Nuclear Factor 3-alphaLungNuclear ProteinsPhosphatidylinositol 3-KinasesPromoter Regions, GeneticProtein IsoformsProteolipidsPulmonary Surfactant-Associated Protein APulmonary Surfactant-Associated ProteinsPulmonary SurfactantsRatsReceptors, Cell SurfaceThyroid Nuclear Factor 1Transcription FactorsTranscription, GeneticConceptsSP-B promoterSP-B transcriptionPI3-kinaseHNF-3Consensus recognition elementSurfactant proteinsPI3-kinase pathwaySP-A receptorGel shift analysisCell transcriptional activityKinase localizationCellular functionsInteraction of SPTranscription factorsCell biologyNuclear localizationPlasma membraneKinase pathwayTranscriptional activityTranscriptionProteinSpCognate receptorsPromoterType II cells
1999
SP‐A as a cytokine: Surfactant protein‐A–regulated transcription of surfactant proteins and other genes
Korutla L, Strayer D. SP‐A as a cytokine: Surfactant protein‐A–regulated transcription of surfactant proteins and other genes. Journal Of Cellular Physiology 1999, 178: 379-386. PMID: 9989784, DOI: 10.1002/(sici)1097-4652(199903)178:3<379::aid-jcp12>3.0.co;2-c.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCarrier ProteinsCells, CulturedFemaleGene Expression RegulationGenes, fosGenes, junMacrophages, PeritonealProteolipidsPulmonary AlveoliPulmonary Surfactant-Associated Protein APulmonary Surfactant-Associated ProteinsPulmonary SurfactantsRatsRats, Sprague-DawleyRNA, MessengerTranscription, GeneticConceptsType II cellsSurfactant secretionProtein gene transcriptionSurfactant proteinsSurfactant gene expressionOverall transcript levelsC-fos transcriptionSP-A receptorII cellsSP-C mRNA levelsHost antimicrobial defenseSurfactant proteins SPMRNA levelsTranscript stabilityGene transcriptionSP-A effectsTranscript levelsGene expressionTranscriptionC-JunAlveolar type II cellsNew transcriptionSurfactant-associated proteinsSpProtein
1997
Surfactant protein-A receptor-mediated inhibition of calcium signaling in alveolar type II cells.
Strayer D, Korutla L, Thomas A. Surfactant protein-A receptor-mediated inhibition of calcium signaling in alveolar type II cells. Receptors & Signal Transduction 1997, 7: 111-20. PMID: 9392439.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsBiological TransportCalciumIonomycinMalePhospholipidsProteolipidsPulmonary AlveoliPulmonary Surfactant-Associated Protein APulmonary Surfactant-Associated ProteinsPulmonary SurfactantsRatsRats, Sprague-DawleyReceptors, Cell SurfaceSignal TransductionThapsigarginConceptsReceptor-mediated inhibitionType II cellsII cellsSP-A receptorSurfactant proteinsType II alveolar cellsReceptor-mediated mechanismAlveolar type II cellsCell membrane receptorsQuantitative fluorescence microscopyAbsence of SPMembrane Ca channelsAction of SPFura-2Membrane receptorsCa storesBiphasic increaseIntracellular storesAlveolar cellsElicit Ca2Physiologic regulatorCalcium ionophoreCa channelsCell membraneIntracellular Ca
1994
Evaluation of Recombinant Platelet Factor 4 and Protamine Sulfate for Heparin Neutralization: Clotting and Clearance Studies in Rat
Korutla L, Stewart G, Lasz E, Maione T, Niewiarowski S. Evaluation of Recombinant Platelet Factor 4 and Protamine Sulfate for Heparin Neutralization: Clotting and Clearance Studies in Rat. Thrombosis And Haemostasis 1994, 71: 609-614. PMID: 8091389, DOI: 10.1055/s-0038-1642491.Peer-Reviewed Original ResearchConceptsRecombinant platelet factor 4Platelet factor 4Protamine sulfatePartial thromboplastin timeFactor 4Heparin clearanceHeparin neutralizationThromboplastin timeVitro additionClearance studiesDifferent physiological effectsAdverse effectsRatsOrgan depositionHeparinAnticoagulant activityPhysiological effectsGreater percentageLoss of radioactivityHalf lifeHeparin-agarose chromatographyInjectionMinLarge percentageRadioactivity