2024
Noninvasive single‐cell‐based prenatal genetic testing: A proof of concept clinical study
Bellair M, Amaral E, Ouren M, Roark C, Kim J, O'Connor A, Soriano A, Schindler M, Wapner R, Stone J, Tavella N, Merriam A, Perley L, Breman A, Beaudet A. Noninvasive single‐cell‐based prenatal genetic testing: A proof of concept clinical study. Prenatal Diagnosis 2024, 44: 304-316. PMID: 38411249, DOI: 10.1002/pd.6529.Peer-Reviewed Original ResearchMeSH KeywordsAmniocentesisAneuploidyFemaleGenetic TestingHumansMosaicismPlacentaPregnancyPrenatal DiagnosisConceptsNoninvasive prenatal genetic testingLow-risk pregnanciesConcept clinical studyCopy number analysisPrenatal genetic testingNext-generation sequencingPlacental mosaicismMaternal bloodAneuploidy resultsMeasures of test performanceCK stainingCVS casesClinical studiesGenetic testingNucleated cellsTrophoblastRed cellsNumber analysisPregnancyBloodDeletions/duplicationsAneuploidyDeletionAmniocentesisCells
2022
Sex differences in early and term placenta are conserved in adult tissues
Olney K, Plaisier S, Phung T, Silasi M, Perley L, O’Bryan J, Ramirez L, Kliman H, Wilson M. Sex differences in early and term placenta are conserved in adult tissues. Biology Of Sex Differences 2022, 13: 74. PMID: 36550527, PMCID: PMC9773522, DOI: 10.1186/s13293-022-00470-y.Peer-Reviewed Original ResearchConceptsFirst trimester placentaLate first trimesterTerm placentaTrimester placentaFirst trimesterAdult tissuesGene expressionSex differencesLifelong health outcomesSex-differential gene expressionGenetic sexInnate immune responseFull-term placentaSex-linked genesInnate immune genesImmune responsePlacenta samplesHealth outcomesBrain tissuePlacentaGametologous genesDifferent timepointsSex chromosomesImmune genesSexInsulin increases placental triglyceride as a potential mechanism for fetal adiposity in maternal obesity
Anam AK, Cooke KM, Dratver MB, O'Bryan JV, Perley LE, Guller SM, Hwang JJ, Taylor HS, Goedeke L, Kliman HJ, Vatner DF, Flannery CA. Insulin increases placental triglyceride as a potential mechanism for fetal adiposity in maternal obesity. Molecular Metabolism 2022, 64: 101574. PMID: 35970449, PMCID: PMC9440306, DOI: 10.1016/j.molmet.2022.101574.Peer-Reviewed Original ResearchConceptsDe novo lipogenesisFetal adiposityFatty acid uptakeMaternal obesityObese womenNormal weightExcess adiposityVillous explantsInsulin receptorNewborns of mothersNormal-weight womenAcid uptakePlacental villous explantsTranscription factor SREBP-1Insulin-like growth factor 2Effect of insulinFatty acid oxidationPlacental triglyceridesPlacental responsesElevated triglyceridesMaternal hyperglycemiaWeight womenGrowth factor 2Diabetes riskPlacental metabolismSeizing the Window of Opportunity Within 1 Year Postpartum: Early Cardiovascular Screening
Ackerman‐Banks C, Grechukhina O, Spatz E, Lundsberg L, Chou J, Smith G, Greenberg VR, Reddy UM, Xu X, O’Bryan J, Smith S, Perley L, Lipkind HS. Seizing the Window of Opportunity Within 1 Year Postpartum: Early Cardiovascular Screening. Journal Of The American Heart Association 2022, 11: e024443. PMID: 35411781, PMCID: PMC9238464, DOI: 10.1161/jaha.121.024443.Peer-Reviewed Original ResearchConceptsHDP groupBody mass indexChronic hypertensionMonths postpartumHypertension 6Metabolic syndromeMass indexYear postpartumFamily historyMedical conditionsNew diagnosisHigh low-density lipoprotein cholesterolLow-density lipoprotein cholesterolEffective primary prevention strategiesPreexisting medical conditionsProspective cohort studyCVD risk scoreUnderlying medical conditionsCardiovascular disease riskMultidisciplinary care modelMultivariable logistic regressionDevelopment of CVDFirst year postpartumPrimary prevention strategiesHypertensive disorders
2017
Early sonographic detection of a succenturiate placenta after IVF in a 42-year-old woman with multiple comorbidities
Stelzl PW, Yadav G, Perley L, Silasi M. Early sonographic detection of a succenturiate placenta after IVF in a 42-year-old woman with multiple comorbidities. BMJ Case Reports 2017, 2017: bcr-2017-222189. PMID: 29021145, PMCID: PMC5652863, DOI: 10.1136/bcr-2017-222189.Peer-Reviewed Original ResearchConceptsPlacental lobeArrest of laborEarly sonographic detectionVelamentous cord insertionAdvanced maternal ageSuccenturiate lobeSuccenturiate placentaMultiple comorbiditiesPlacenta previaVaginal bleedingCaesarean sectionCord insertionElective inductionHypercoagulable stateVasa previaWeeks' gestationSingleton placentasMaternal ageMedical historySonographic detectionSpontaneous abortionHealthy girlsUmbilical cordGross examinationPlacentaTime course analysis of RNA quality in placenta preserved by RNAlater or flash freezing
Martin NM, Cooke KM, Radford CC, Perley LE, Silasi M, Flannery CA. Time course analysis of RNA quality in placenta preserved by RNAlater or flash freezing. American Journal Of Reproductive Immunology 2017, 77 PMID: 28138995, PMCID: PMC5397349, DOI: 10.1111/aji.12637.Peer-Reviewed Original Research