Dietary protein increases intestinal calcium absorption in part by increasing tight junction expression of Claudin‐2
Gaffney‐Stomberg E, Cucchi C, Sun B, Bataille A, Renfro L, Kerstetter J, Insogna K. Dietary protein increases intestinal calcium absorption in part by increasing tight junction expression of Claudin‐2. The FASEB Journal 2011, 25: 983.30-983.30. DOI: 10.1096/fasebj.25.1_supplement.983.30.Peer-Reviewed Original ResearchHigh-protein dietCaco-2 BBe cellsClaudin-2 expressionCa absorptionCalcium absorptionProtein dietClaudin-2BBE cellsFemale Sprague-Dawley ratsIntestinal calcium absorptionDietary proteinSprague-Dawley ratsTight junction expressionCa transportTight junction proteinsTranscellular Ca transportDietary protein effectsCa-transporting proteinsDuodenal mucosaAcute effectsDay 7MRNA expressionJunction expressionJunction proteinsAmino acidsDietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression
Cucchi C, Gaffney‐Stomberg E, Sun B, O'Brien K, Kerstetter J, Insogna K. Dietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression. The FASEB Journal 2011, 25: lb193-lb193. DOI: 10.1096/fasebj.25.1_supplement.lb193.Peer-Reviewed Original ResearchProtein dietDietary proteinProtein expressionCaco-2 BBe cellsIntestinal Fe absorptionFe absorption studiesIntestinal iron absorptionTranscript expressionAmino acid-free mediumDMT1 protein levelsAmino acid treatmentDMT1 protein expressionProtein doseIron absorptionBBE cellsIron deficiencyAcid-free mediumRatsProtein levelsDietDMT1 transcriptsAmino acidsFe absorptionWestern analysisKey regulator