2000
Dihydrofolate Reductase from Kaposi's Sarcoma-Associated Herpesvirus
Cinquina C, Grogan E, Sun R, Lin S, Beardsley G, Miller G. Dihydrofolate Reductase from Kaposi's Sarcoma-Associated Herpesvirus. Virology 2000, 268: 201-217. PMID: 10683342, DOI: 10.1006/viro.1999.0165.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusDihydrofolate reductaseEarly lytic cycle genesNucleotide metabolism genesDHFR activityHuman DHFRViral enzymesPrimary effusion lymphomaLytic cycle genesKSHV lytic cycleMethionine biosynthesisCycle genesHuman dihydrofolate reductaseCultured B-cell linesMetabolism genesPEL cell linesOwn enzymesB cell linesAmino acidsPEL cellsChemical inductionLytic cycleE. coliInfected cells
1999
Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes
Ragoczy T, Miller G. Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes. Journal Of Virology 1999, 73: 9858-9866. PMID: 10559298, PMCID: PMC113035, DOI: 10.1128/jvi.73.12.9858-9866.1999.Peer-Reviewed Original ResearchConceptsLytic cycle genesRaji cellsEpstein–Barr virus Rta proteinEpstein-Barr virus (EBV) lytic cycleViral targetsLytic cycleVirus lytic cycleLymphoma cell line RajiBurkitt's lymphoma cell line RajiBZLF1 expressionB cell linesCell line RajiBRLF1 geneImmediate early genesInhibitory effectCycle genesViral lytic cycle genesEBVRta proteinViral genesBLRF2CellsExpressionTransactivation functionLate genesAmino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator
Francis A, Ragoczy T, Gradoville L, Heston L, El-Guindy A, Endo Y, Miller G. Amino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator. Journal Of Virology 1999, 73: 4543-4551. PMID: 10233912, PMCID: PMC112494, DOI: 10.1128/jvi.73.6.4543-4551.1999.Peer-Reviewed Original ResearchConceptsLytic cycle genesLatent virusZEBRA proteinOverexpression of RtaEpstein-Barr virusLatent EBV genomeBRLF1 expressionEarly lytic cycle genesAmino acid substitutionsEBV genomeBRLF1BRLF1 promoterCycle genesAcid substitutionsVirusEpstein-Barr virus R transactivatorSerine 186Distinct phenotypesLytic life cycleR transactivatorActivationDifferent amino acid substitutionsResponse elementS186ExpressionCellular Tropism and Viral Interleukin-6 Expression Distinguish Human Herpesvirus 8 Involvement in Kaposi’s Sarcoma, Primary Effusion Lymphoma, and Multicentric Castleman’s Disease
Staskus K, Sun R, Miller G, Racz P, Jaslowski A, Metroka C, Brett-Smith H, Haase A. Cellular Tropism and Viral Interleukin-6 Expression Distinguish Human Herpesvirus 8 Involvement in Kaposi’s Sarcoma, Primary Effusion Lymphoma, and Multicentric Castleman’s Disease. Journal Of Virology 1999, 73: 4181-4187. PMID: 10196314, PMCID: PMC104197, DOI: 10.1128/jvi.73.5.4181-4187.1999.Peer-Reviewed Original ResearchConceptsViral gene expressionMulticentric Castleman's diseasePrimary effusion lymphomaLevel of expressionCell typesGene expressionViral genesKaposi's sarcomaInfected cell typesLytic cycle viral gene expressionHeterogeneous cell typesCastleman's diseaseInterleukin-6Lytic cycle genesEffusion lymphomaHuman immunodeficiency virus-positive individualsPathogenesis of PELPredominant infected cell typeHuman herpesvirus 8 infectionViral homologueVirus-positive individualsVIL-6B cell growthAltered expressionGenes
1998
The Epstein-Barr Virus Rta Protein Activates Lytic Cycle Genes and Can Disrupt Latency in B Lymphocytes
Ragoczy T, Heston L, Miller G. The Epstein-Barr Virus Rta Protein Activates Lytic Cycle Genes and Can Disrupt Latency in B Lymphocytes. Journal Of Virology 1998, 72: 7978-7984. PMID: 9733836, PMCID: PMC110133, DOI: 10.1128/jvi.72.10.7978-7984.1998.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesBase SequenceCell LineChloramphenicol O-AcetyltransferaseDNA PrimersDNA ReplicationDNA-Binding ProteinsGene Expression Regulation, ViralHerpesvirus 4, HumanHumansImmediate-Early ProteinsPromoter Regions, GeneticTrans-ActivatorsTranscription FactorsViral ProteinsVirus LatencyConceptsEpstein-Barr virusLytic cycle genesB lymphocytesEpstein–Barr virus Rta proteinEpithelial cellsLytic cycleDisruption of latencyViral lytic cycleB cell linesEBV entryImmediate early viral genesBZLF1LymphocytesCycle genesExpression of RTARTA functionBRLF1Rta proteinDownstream targetsViral genesViral DNA replicationExpressionCellsA viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus
Sun R, Lin S, Gradoville L, Yuan Y, Zhu F, Miller G. A viral gene that activates lytic cycle expression of Kaposi’s sarcoma-associated herpesvirus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10866-10871. PMID: 9724796, PMCID: PMC27987, DOI: 10.1073/pnas.95.18.10866.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCloning, MolecularDNA PrimersDNA, ComplementaryGene Expression Regulation, NeoplasticGenes, ViralHerpesvirus 4, HumanHerpesvirus 8, HumanHumansMolecular Sequence DataSarcoma, KaposiSequence Homology, Amino AcidTranscription, GeneticTumor Cells, CulturedVirus ActivationConceptsEpstein-Barr virusKaposi's sarcoma-associated herpesvirusSarcoma-associated herpesvirusSmall viral capsid antigenPathogenesis of KSHVLytic cycle gene expressionViral capsid antigenLatent viral genomeCapsid antigenLytic cycle genesInterleukin-6Immediate early genesViral cytokineEarly lytic genesLytic genesHerpesvirusVirusViral genesViral genomeExpressionGene expressionCytokinesPathogenesisCycle genesGenes