1998
[16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry
Rudnick G. [16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry. Methods In Enzymology 1998, 296: 233-247. PMID: 9779452, DOI: 10.1016/s0076-6879(98)96018-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNumber of ionsKinetic determinationMolecular machinesCharge movesIonsLipid bilayersNeurotransmitter moleculesMoleculesInput of energySteady-state distributionLow concentrationsChargeElectrical potentialMembraneIon gradientsStoichiometryHigh concentrationsUltimate concentrationNaConcentrationEnergyBilayersNeurotransmitter transportersNumber of NaNeurotransmitter transport
1996
Ion Coupling Stoichiometry for the Norepinephrine Transporter in Membrane Vesicles from Stably Transfected Cells (∗)
Gu H, Wall S, Rudnick G. Ion Coupling Stoichiometry for the Norepinephrine Transporter in Membrane Vesicles from Stably Transfected Cells (∗). Journal Of Biological Chemistry 1996, 271: 6911-6916. PMID: 8636118, DOI: 10.1074/jbc.271.12.6911.Peer-Reviewed Original ResearchConceptsMembrane vesiclesTransmembrane ion gradientsLLC-PK1 cellsMajor substrateTransport substratesTransfected CellsStably Transfected CellsIon gradientsCoupling stoichiometryNet positive chargeDA accumulationVesiclesSubstrate moleculesTransportersNorepinephrine transporterAccumulationCellsGamma-aminobutyric acidCotransportDAGradientStoichiometrySubstrateAbsenceTransport processes