2002
Chemical Modification Strategies for Structure‐Function Studies
Rudnick G. Chemical Modification Strategies for Structure‐Function Studies. 2002, 125-141. DOI: 10.1002/0471434043.ch8.ChaptersTransmembrane domainConformational changesPolytopic membrane proteinsStructure-function studiesMembrane proteinsTransporter proteinsReactive residuesLipid bilayersProteinTransportersExternal faceExternal loopChemical modification strategiesDomainChemical modificationSubstrateCysteineResiduesSitesMembraneModificationBilayers
1996
Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells*
Gu H, Ahn J, Caplan M, Blakely R, Levey A, Rudnick G. Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells*. Journal Of Biological Chemistry 1996, 271: 18100-18106. PMID: 8663573, DOI: 10.1074/jbc.271.30.18100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiogenic AminesBiological TransportCarrier ProteinsCell CompartmentationCell MembraneCell PolarityCells, CulturedDogsDopamineDopamine Plasma Membrane Transport ProteinsEpithelial CellsHumansImmunohistochemistryMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsRatsRecombinant ProteinsSerotoninSerotonin Plasma Membrane Transport ProteinsSymportersConceptsMadin-Darby canine kidneyMDCK cellsLLC-PK1 cellsNeurotransmitter transportersCell surface biotinylationConfocal immunofluorescence microscopyBasolateral membraneCell-specific mechanismsEpithelial cellsBiogenic amine transportersMembrane proteinsSurface biotinylationCDNA encodingHuman DA transporterAmine transportersImmunofluorescence microscopyBiotinylating reagentTransportersPermeable filter supportsApical surfaceImmunocytochemistry resultsBasolateral mediumSurface expressionApical sideDA transporter
1987
Hydrodynamic properties of the chromaffin granule hydrogen ion pumping adenosinetriphosphatase.
Dean G, Nelson P, Agnew W, Rudnick G. Hydrodynamic properties of the chromaffin granule hydrogen ion pumping adenosinetriphosphatase. Biochemistry 1987, 26: 2301-5. PMID: 2887199, DOI: 10.1021/bi00382a034.Peer-Reviewed Original ResearchConceptsGel permeation chromatographyCm3/Permeation chromatographyHydrodynamic propertiesSedimentation equilibrium centrifugationMixed micellesHydrogen ionsMicellesStokes radiusBovine adrenal chromaffin granulesIntrinsic membrane proteinsDetergentsParticlesParticle volumePropertiesIonsEquilibrium centrifugationChromatographyMitochondrial F1F0-ATPaseAdrenal chromaffin granulesDaltonsProtein componentsMembrane proteinsProtein V
1979
Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport.
Talvenheimo J, Nelson P, Rudnick G. Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport. Journal Of Biological Chemistry 1979, 254: 4631-4635. PMID: 438209, DOI: 10.1016/s0021-9258(17)30057-1.Peer-Reviewed Original Research
1976
Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli.
Rudnick G, Schuldiner S, Kaback H. Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli. Biochemistry 1976, 15: 5126-31. PMID: 791364, DOI: 10.1021/bi00668a028.Peer-Reviewed Original ResearchConceptsLac carrier proteinMembrane vesiclesCarrier proteinY gene productEscherichia coli ML 308P-nitrophenyl alphaD-lactateMembrane proteinsGene productsML 308Cryptic formVesicle membraneLactose transportElectrochemical gradientEscherichia coliEnergy couplingProteinVesiclesCarbonyl cyanideSimilar affinityCompetitive inhibitorHigh affinity formMembraneBindingFlow dialysis