2011
Deficient CX3CR1 Signaling Promotes Recovery after Mouse Spinal Cord Injury by Limiting the Recruitment and Activation of Ly6Clo/iNOS+ Macrophages
Donnelly DJ, Longbrake EE, Shawler TM, Kigerl KA, Lai W, Tovar CA, Ransohoff RM, Popovich PG. Deficient CX3CR1 Signaling Promotes Recovery after Mouse Spinal Cord Injury by Limiting the Recruitment and Activation of Ly6Clo/iNOS+ Macrophages. Journal Of Neuroscience 2011, 31: 9910-9922. PMID: 21734283, PMCID: PMC3139517, DOI: 10.1523/jneurosci.2114-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAntigens, LyCD11 AntigensCells, CulturedChemokine CXCL1CX3C Chemokine Receptor 1Disease Models, AnimalFlow CytometryGene Expression RegulationGreen Fluorescent ProteinsMacrophagesMiceMice, Inbred C57BLMice, TransgenicMotor ActivityMyelin Basic ProteinNitric OxideNitric Oxide Synthase Type IIReceptors, ChemokineRecovery of FunctionSignal TransductionSpinal Cord InjuriesConceptsSpinal cord injuryMonocyte-derived macrophagesCord injuryMouse spinal cord injuryTraumatic spinal cord injuryBlockade of CX3CR1Novel monocyte subsetsCX3CR1-deficient miceAnti-inflammatory therapySelective anti-inflammatory therapyWild-type miceDistinct macrophage subsetsInflammatory cytokinesMonocyte subsetsChemokine receptorsFunctional improvementSpinal cordInflammatory signalingNeurotoxic effectsPromotes recoveryMacrophage subsetsLesion siteCX3CR1Oxidative metabolitesMice
2007
Characterization and modeling of monocyte‐derived macrophages after spinal cord injury
Longbrake EE, Lai W, Ankeny DP, Popovich PG. Characterization and modeling of monocyte‐derived macrophages after spinal cord injury. Journal Of Neurochemistry 2007, 102: 1083-1094. PMID: 17663750, DOI: 10.1111/j.1471-4159.2007.04617.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB7-1 AntigenCD11b AntigenCytokinesDisease Models, AnimalFemaleFlow CytometryGene Expression RegulationHistocompatibility Antigens Class IIIn Vitro TechniquesMacrophagesMiceMice, Inbred C57BLPhagocytosisReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSpinal Cord InjuriesTime FactorsConceptsSpinal cord injuryMonocyte-derived macrophagesBone marrow-derived macrophagesCord injurySpinal cordContext of SCIMarrow-derived macrophagesNeuroinflammatory reactionThioglycollate-elicited peritonealEffector cellsPeripheral macrophagesResident peritonealCytokine expressionLaser capture microdissectionReal-time PCRMacrophage subsetsMDM differentiationInjuryMacrophagesCapture microdissectionCordPeritonealMDM responseFunctional consequencesFuture studies