1999
The Length of the Flexible SNAREpin Juxtamembrane Region Is a Critical Determinant of SNARE-Dependent Fusion
McNew J, Weber T, Engelman D, Söllner T, Rothman J. The Length of the Flexible SNAREpin Juxtamembrane Region Is a Critical Determinant of SNARE-Dependent Fusion. Molecular Cell 1999, 4: 415-421. PMID: 10518222, DOI: 10.1016/s1097-2765(00)80343-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, SurfaceCarrier ProteinsMembrane FusionMembrane ProteinsMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsPliabilityProlineProtein Structure, SecondaryRecombinant ProteinsR-SNARE ProteinsSNARE ProteinsSynaptosomal-Associated Protein 25Syntaxin 1Vesicular Transport ProteinsConceptsJuxtamembrane regionMembrane fusionSNARE-dependent membrane fusionSNARE-dependent fusionHelix-breaking proline residueSNARE proteinsTransmembrane domainSyntaxin 1ACoil domainProline residuesFlexible linkerLipid bilayersCritical determinantFusion efficiencyFusionVAMPDomainProteinRate of fusionSnareVesiclesResiduesLinkerSame changesRegion
1997
Assessment of the aggregation state of integral membrane proteins in reconstituted phospholipid vesicles using small angle neutron scattering11Edited by M. F. Moody
Hunt J, McCrea P, Zaccaı̈ G, Engelman D. Assessment of the aggregation state of integral membrane proteins in reconstituted phospholipid vesicles using small angle neutron scattering11Edited by M. F. Moody. Journal Of Molecular Biology 1997, 273: 1004-1019. PMID: 9367787, DOI: 10.1006/jmbi.1997.1330.Peer-Reviewed Original ResearchConceptsMembrane protein complexesIntegral membrane proteinsProtein complexesMembrane proteinsIntegral membrane protein complexPhospholipid vesiclesSmall unilamellar phospholipid vesiclesUnilamellar phospholipid vesiclesMolecular massF. MoodySpatial arrangementNon-ionic detergentIndividual complexesVesiclesModel systemMonomeric bacteriorhodopsinProteinUnknown scopeComplexesAggregation stateRadius of gyrationBacteriorhodopsinDetergentsBilayers
1987
Transmembrane topography of the nicotinic acetylcholine receptor delta subunit.
McCrea P, Popot J, Engelman D. Transmembrane topography of the nicotinic acetylcholine receptor delta subunit. The EMBO Journal 1987, 6: 3619-3626. PMID: 3428268, PMCID: PMC553829, DOI: 10.1002/j.1460-2075.1987.tb02693.x.Peer-Reviewed Original ResearchConceptsDisulfide bridgesAcetylcholine receptor delta subunitIntermolecular disulfide bridgesTransmembrane topographyTransmembrane segmentsTransmembrane crossingReceptor delta subunitCellular locationC-terminusN-terminusDelta subunitNicotinic acetylcholine receptorsSubunitsElectric organVesiclesPermeability barrierTorpedo marmorataVesicle systemAcetylcholine receptorsDiphtheria toxinAqueous spaceDimers