2008
The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival. Journal Of Surgical Research 2008, 155: 125-131. PMID: 19059608, DOI: 10.1016/j.jss.2008.07.029.Peer-Reviewed Original ResearchConceptsERK 1/2P38 MAPKProtein kinaseKeratinocyte proliferation rateCell deathAkt inhibitorCyclic stretchDNA synthesisAktTransferase-mediated dUTP nick-end labeling stainingRepetitive stretchApoptosisMAPKDUTP nick end labeling stainingProliferation rateHuman dermalCell numberNick end labeling stainingTUNEL-positive cellsDermal keratinocytesStretchTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingBrdU stainingKeratinocyte proliferationProliferation
2007
Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival. Wound Repair And Regeneration 2007, 15: 646-656. PMID: 17971010, DOI: 10.1111/j.1524-475x.2007.00295.x.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMAPK/ERK kinaseExtracellular signal-regulated kinaseDominant-negative AktHuman dermal fibroblastsSignal-regulated kinaseCell numberERK kinaseProtein kinaseTransduction pathwaysDermal fibroblastsRelevant transduction pathwaysRelevant signal pathwaysDermal fibroblast proliferationHuman dermal fibroblast proliferationCultured human dermal fibroblastsSurvival of fibroblastsSignal pathwayKinaseNecrotic fibroblastsDNA synthesisCell proliferationAktTransferase-mediated dUTP nick-end labeling staining
2003
Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation
Haga M, Yamashita A, Paszkowiak J, Sumpio BE, Dardik A. Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation. Journal Of Vascular Surgery 2003, 37: 1277-1284. PMID: 12764276, DOI: 10.1016/s0741-5214(03)00329-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Physiological PhenomenaDisease Models, AnimalHemodynamicsIn Vitro TechniquesMyocytes, Smooth MuscleOscillometryPhosphatidylinositol 3-KinasesPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthSignal TransductionStress, PhysiologicalVascular DiseasesConceptsSignal transduction pathwaysAkt phosphorylationTransduction pathwaysPI3K-Akt signal transduction pathwaySmooth muscle cell proliferationMuscle cell proliferationCell proliferationControl cellsOscillatory shear stressMaximal phosphorylationPI3K inhibitorsCell numberSurvival pathwaysAkt activationBovine aortic SMCSMC survivalInhibitor LY294002Akt pathwayPhosphorylationWestern blot techniqueControl survivalDNA synthesisK inhibitorsAortic SMCsPathway
1993
Control of smooth muscle cell proliferation by psoralen photochemotherapy
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-1018. DOI: 10.1016/0741-5214(93)90671-8.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplastyControl of smooth muscle cell proliferation by psoralen photochemotherapy.
Sumpio B, Phan S, Gasparro F, Deckelbaum L. Control of smooth muscle cell proliferation by psoralen photochemotherapy. Journal Of Vascular Surgery 1993, 17: 1010-6; discussion 1016-8. PMID: 8505779, DOI: 10.1067/mva.1993.45747.Peer-Reviewed Original ResearchConceptsSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationPsoralen photochemotherapyPeripheral vascular diseaseLong-term therapyCell proliferationInhibits SMC proliferationTrypan blue exclusionBypass graftDistal anastomosisBalloon angioplastyUVA photochemotherapyVascular diseaseIntimal hyperplasiaGrowth of SMCsBlue exclusionPhotochemotherapySMC viabilityTime courseProliferationUVADNA synthesisDaysAngioplasty