2023
Cytokinopathy with aberrant cytotoxic lymphocytes and profibrotic myeloid response in SARS-CoV-2 mRNA vaccine–associated myocarditis
Barmada A, Klein J, Ramaswamy A, Brodsky N, Jaycox J, Sheikha H, Jones K, Habet V, Campbell M, Sumida T, Kontorovich A, Bogunovic D, Oliveira C, Steele J, Hall E, Pena-Hernandez M, Monteiro V, Lucas C, Ring A, Omer S, Iwasaki A, Yildirim I, Lucas C. Cytokinopathy with aberrant cytotoxic lymphocytes and profibrotic myeloid response in SARS-CoV-2 mRNA vaccine–associated myocarditis. Science Immunology 2023, 8: eadh3455-eadh3455. PMID: 37146127, PMCID: PMC10468758, DOI: 10.1126/sciimmunol.adh3455.Peer-Reviewed Original ResearchConceptsMRNA vaccinesSARS-CoV-2 mRNA vaccinesSARS-CoV-2 mRNA vaccinationC-reactive protein levelsB-type natriuretic peptidePeripheral blood mononuclear cellsCardiac tissue inflammationDeep immune profilingSerum soluble CD163Vaccine-associated myocarditisCohort of patientsBlood mononuclear cellsCytotoxic T cellsLate gadolinium enhancementHypersensitivity myocarditisElevated troponinMRNA vaccinationImaging abnormalitiesNK cellsImmune profilingKiller cellsMyeloid responseNatriuretic peptideHumoral mechanismsInflammatory cytokines
2022
Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
Godoy-Tena G, Barmada A, Morante-Palacios O, de la Calle-Fabregat C, Martins-Ferreira R, Ferreté-Bonastre A, Ciudad L, Ruiz-Sanmartín A, Martínez-Gallo M, Ferrer R, Ruiz-Rodriguez J, Rodríguez-Ubreva J, Vento-Tormo R, Ballestar E. Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines. Genome Medicine 2022, 14: 134. PMID: 36443794, PMCID: PMC9706884, DOI: 10.1186/s13073-022-01137-4.Peer-Reviewed Original ResearchConceptsDNA methylation alterationsSevere COVID-19 patientsInterferon-related genesCOVID-19 patientsCell typesMethylation alterationsMyeloid differentiationSingle-cell transcriptomesSingle-cell transcriptomicsDNA methylation changesGene expression changesPeripheral blood monocytesImmune cell typesMethylationEPIC BeadChip arraySpecific DNA methylation alterationsTranscriptional reprogrammingDNA methylomeTranscriptomic reprogrammingDNA methylationInflammatory cytokinesMethylation changesEpigenetic alterationsBlood monocytesExpression changesBeadChip array
2021
Maximizing insights from monogenic immune disorders
Barmada A, Ramaswamy A, Lucas CL. Maximizing insights from monogenic immune disorders. Current Opinion In Immunology 2021, 73: 50-57. PMID: 34695727, PMCID: PMC8915947, DOI: 10.1016/j.coi.2021.09.008.Peer-Reviewed Original ResearchConceptsDisease gene discoveryGene discoverySingle geneIntegrative omicsMonogenic immune disordersHuman diseasesUnprecedented insightsMouse modelingFundamental immune functionsImmune disordersMechanistic studiesGenomeGenomicsImmune functionOmicsDisease discoveryDiscoveryGenesInsightsDisordersDisease
2020
Quantifying Sample Collection and Processing Impacts on Fiber-Based Tear Fluid Chemical Analysis
Barmada A, Shippy S. Quantifying Sample Collection and Processing Impacts on Fiber-Based Tear Fluid Chemical Analysis. Translational Vision Science & Technology 2020, 9: 23-23. PMID: 33024616, PMCID: PMC7521181, DOI: 10.1167/tvst.9.10.23.Peer-Reviewed Original ResearchTear analysis as the next routine body fluid test
Barmada A, Shippy S. Tear analysis as the next routine body fluid test. Eye 2020, 34: 1731-1733. PMID: 32376979, PMCID: PMC7608258, DOI: 10.1038/s41433-020-0930-0.Peer-Reviewed Original Research