2023
Mephedrone induces partial release at human dopamine transporters but full release at human serotonin transporters
Mayer F, Niello M, Bulling S, Zhang Y, Li Y, Kudlacek O, Holy M, Kooti F, Sandtner W, Rudnick G, Schmid D, Sitte H. Mephedrone induces partial release at human dopamine transporters but full release at human serotonin transporters. Neuropharmacology 2023, 240: 109704. PMID: 37703919, DOI: 10.1016/j.neuropharm.2023.109704.Peer-Reviewed Original ResearchSerotonin transporterCarrier-mediated releaseProtein kinase C inhibitor GF109203X.Human embryonic kidney 293Empathogenic effectsAbuse liabilityGreater efficacyElectrophysiological recordingsDopamine transporterHuman DATReverse transportEntactogenic effectsStimulating propertiesHuman serotonin transporterHuman dopamine transporterMephedroneDopamineMolecular mechanismsHDATReleaserTransportersHigh-affinity transporterDATReleasePrevious studies
2001
The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells
Gu H, Wu X, Giros B, Caron M, Caplan M, Rudnick G. The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells. Molecular Biology Of The Cell 2001, 12: 3797-3807. PMID: 11739781, PMCID: PMC60756, DOI: 10.1091/mbc.12.12.3797.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineCell PolarityDogsDopamine Plasma Membrane Transport ProteinsEpithelial CellsHumansMembrane GlycoproteinsMembrane Transport ProteinsMiceMicroscopy, ConfocalMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsNorepinephrine Plasma Membrane Transport ProteinsProtein Sorting SignalsSequence AlignmentSymportersConceptsBasolateral localization signalLocalization signalDileucine motifPlasma membraneBasolateral localizationOverall amino acid sequence identityAmino acid sequence identityTerminal regionMDCK cellsApical plasma membraneBasolateral membraneEpithelial cellsSequence identityApical localizationChimeric proteinTransport assaysTransporter localizationAmino acidsApical membraneNorepinephrine transporterTransportersCorresponding sequenceDopamine transporterSame mutationMembrane
1995
Biogenic amine flux mediated by cloned transporters stably expressed in cultured cell lines: amphetamine specificity for inhibition and efflux.
Wall S, Gu H, Rudnick G. Biogenic amine flux mediated by cloned transporters stably expressed in cultured cell lines: amphetamine specificity for inhibition and efflux. Molecular Pharmacology 1995, 47: 544-50. PMID: 7700252.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-4-phenylpyridiniumAmphetaminesBiogenic MonoaminesBiological TransportCarrier ProteinsCell MembraneCells, CulturedCloning, MolecularCocaineDNA, ComplementaryDopamineDopamine Plasma Membrane Transport ProteinsHumansMazindolMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNeurotransmitter Uptake InhibitorsNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsSerotoninSerotonin Plasma Membrane Transport ProteinsStimulation, ChemicalSubstrate SpecificitySymportersTransfectionConceptsBiogenic amine transportersCell linesAmine transportersRat serotonin transporterCultured cell linesInhibitor of transportRat dopamine transporterHuman norepinephrine transporterPlasma membraneLLC-PK1 cellsSubstrate effluxSubstrate influxDopamine transporterNorepinephrine transporterAmphetamine derivativesTransportersSerotonin transporterEffluxDistinct patternsP-chloroamphetamineAmine substratesCellsInhibited transportCDNAInhibitors
1993
Binding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding.
Wall S, Innis R, Rudnick G. Binding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding. Molecular Pharmacology 1993, 43: 264-70. PMID: 8429827.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveBlood PlateletsCarrier ProteinsCocaineCorpus StriatumDopamineDopamine Plasma Membrane Transport ProteinsHydrogen-Ion ConcentrationIn Vitro TechniquesIonsMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsOsmolar ConcentrationProtein BindingRadioligand AssayRatsSerotoninSerotonin Plasma Membrane Transport ProteinsNon-neurotoxic amphetamine derivatives release serotonin through serotonin transporters.
Rudnick G, Wall S. Non-neurotoxic amphetamine derivatives release serotonin through serotonin transporters. Molecular Pharmacology 1993, 43: 271-6. PMID: 8429828.Peer-Reviewed Original ResearchMeSH KeywordsAmphetaminesBlood PlateletsCarrier ProteinsCell MembraneChromaffin GranulesHumansImipramineIn Vitro TechniquesIndansMembrane GlycoproteinsMembrane Transport ProteinsMethamphetamineModels, BiologicalNerve EndingsNerve Tissue ProteinsRadioligand AssaySerotoninSerotonin Plasma Membrane Transport ProteinsConceptsChromaffin granule membrane vesiclesPlasma membrane vesiclesMembrane vesiclesPlatelet plasma membrane vesiclesPlasma membrane transportersSerotonin transportBiogenic amine transportersMembrane transportersAmine transportersTransportersVesiclesDopamine transporterSerotonin transporterModel systemHalf-maximal concentrationNerve terminalsTransmembraneCocaine analogBindingSimilar concentrationsHigh concentrationsCellsTransport