2008
Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia
Guller S, Buhimschi CS, Y Y, Huang T, Yang L, Kuczynski E, Zambrano E, Lockwood CJ, Buhimschi IA. Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia. Laboratory Investigation 2008, 88: 1057-1067. PMID: 18679377, PMCID: PMC2682720, DOI: 10.1038/labinvest.2008.74.Peer-Reviewed Original ResearchConceptsPlasminogen activator inhibitor-1Severe preeclampsiaReperfusion injurySFlt-1Soluble fms-like tyrosine kinase-1Fms-like tyrosine kinase-1Ischemia/reperfusion injuryLife-threatening syndromeSubsequent reperfusion injuryTerm control groupPathophysiology of preeclampsiaTyrosine kinase-1Activator inhibitor-1Release of factorsSignificant increasePresence of mRNAUpregulation of mRNACeruloplasmin mRNAPlacental damagePlacental factorsMicroarray gene profilingEndothelium dysfunctionPE placentasQuantitative real-time PCRIntervillous space
2007
Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line
Meade ES, Ma YY, Guller S. Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line. Placenta 2007, 28: 1012-1019. PMID: 17570486, PMCID: PMC2001228, DOI: 10.1016/j.placenta.2007.04.005.Peer-Reviewed Original ResearchConceptsPAI-1 expressionHTR-8/SVneo cellsPlasminogen activator inhibitorTrophoblast cell linePAI-1HIF-1alphaHIF-2alphaFirst trimester extravillous trophoblast cell lineHuman trophoblast cell linePlasmin-dependent fibrinolysisExtravillous trophoblast cell linePlasminogen activator inhibitor-1 expressionHypoxia-inducible transcription factor-1αHypoxic conditionsPAI-1 protein levelsCell linesPAI-1 mRNAEarly pregnancyExtracellular matrix degradationHypoxia-inducible transcription factorsQuantitative real-time PCRReal-time PCRActivator inhibitorFactor-1αWestern blottingContrasting effects of chronic hypoxia and nitric oxide synthase inhibition on circulating angiogenic factors in a rat model of growth restriction
Bahtiyar MO, Buhimschi C, Ravishankar V, Copel J, Norwitz E, Julien S, Guller S, Buhimschi IA. Contrasting effects of chronic hypoxia and nitric oxide synthase inhibition on circulating angiogenic factors in a rat model of growth restriction. American Journal Of Obstetrics And Gynecology 2007, 196: 72.e1-72.e6. PMID: 17240241, DOI: 10.1016/j.ajog.2006.07.048.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChronic DiseaseDisease Models, AnimalEnzyme InhibitorsFemaleFetal Growth RetardationHypoxiaNG-Nitroarginine Methyl EsterNitric Oxide SynthasePlacenta Growth FactorPregnancyPregnancy ProteinsRatsRats, Sprague-DawleyVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth FactorsConceptsVascular endothelial growth factorL-NAMESerum levelsChronic hypoxiaSoluble fms-like tyrosine kinase-1Nitro-L-arginine methyl esterFms-like tyrosine kinase-1Adult Sprague-Dawley ratsGrowth factorMaternal serum levelsFree serum levelsIntrauterine growth restrictionOnset of laborPlacental growth factorTyrosine kinase-1Subcutaneous osmotic pumpsSprague-Dawley ratsNitric oxide inhibitionEndothelial growth factorNonpregnant controlsSFlt-1Growth restrictionHypoxic chamberMaternal bloodOxide inhibition