2023
Different perspectives on women’s health, nutrition and endurance exercise
Charkoudian N, Lee J, Giersch G, DiPietro L, Stachenfeld N. Different perspectives on women’s health, nutrition and endurance exercise. Journal Of The International Society Of Sports Nutrition 2023, 20: 2286286. PMID: 38018805, PMCID: PMC11018311, DOI: 10.1080/15502783.2023.2286286.Peer-Reviewed Original ResearchGuidelines on the use of sex and gender in cardiovascular research
Usselman C, Lindsey M, Robinson A, Habecker B, Taylor C, Merryman W, Kimmerly D, Bender J, Regensteiner J, Moreau K, Pilote L, Wenner M, O'Brien M, Yarovinsky T, Stachenfeld N, Charkoudian N, Denfeld Q, Moreira-Bouchard J, Pyle W, DeLeon-Pennell K. Guidelines on the use of sex and gender in cardiovascular research. AJP Heart And Circulatory Physiology 2023, 326: h238-h255. PMID: 37999647, PMCID: PMC11219057, DOI: 10.1152/ajpheart.00535.2023.Peer-Reviewed Original ResearchThe Biological Basis of Sex Differences in Athletic Performance: Consensus Statement for the American College of Sports Medicine
HUNTER S, ANGADI S, BHARGAVA A, HARPER J, HIRSCHBERG A, LEVINE B, MOREAU K, NOKOFF N, STACHENFELD N, BERMON S. The Biological Basis of Sex Differences in Athletic Performance: Consensus Statement for the American College of Sports Medicine. Medicine & Science In Sports & Exercise 2023, 55: 2328-2360. PMID: 37772882, DOI: 10.1249/mss.0000000000003300.Peer-Reviewed Original ResearchConceptsSex steroid hormonesConsensus statementAthletic performanceEndogenous sex steroid hormonesSex differencesSteroid hormonesOnset of pubertyExercise trainingMuscle strengthAcute boutFundamental sex differencesSex hormonesAmerican CollegeParticular testosteroneAdult menSports medicineTraining statusHigh-impact studiesHormoneSimilar ageNonphysiological factorsPrimary determinantMalesBiological basisWomenComment on “Effects of biological sex and oral contraceptive pill use on cutaneous microvascular endothelial function and nitric oxide-dependent vasodilation in humans”
Colburn A, Stachenfeld N. Comment on “Effects of biological sex and oral contraceptive pill use on cutaneous microvascular endothelial function and nitric oxide-dependent vasodilation in humans”. Journal Of Applied Physiology 2023, 135: 485-486. PMID: 37541850, DOI: 10.1152/japplphysiol.00335.2023.Peer-Reviewed Original ResearchTestosterone-associated blood pressure dysregulation in women with androgen excess polycystic ovary syndrome
Stone T, Yanes Cardozo L, Oluwatade T, Leone C, Burgos M, Okifo F, Pal L, Reckelhoff J, Stachenfeld N. Testosterone-associated blood pressure dysregulation in women with androgen excess polycystic ovary syndrome. AJP Heart And Circulatory Physiology 2023, 325: h232-h243. PMID: 37327000, PMCID: PMC10393337, DOI: 10.1152/ajpheart.00164.2023.Peer-Reviewed Original ResearchConceptsSympathetic nervous system activityBlood pressure dysregulationPolycystic ovary syndromeBody mass indexBaroreflex gainOvary syndromeBlood pressureObese insulin-resistant womenPolycystic ovary syndrome phenotypesRenin-angiotensin system activationSerum angiotensin-converting enzymeGonadotropin-releasing hormone antagonistLower sympathetic nervous system activityLower body negative pressureHeightened cardiovascular riskInsulin-resistant womenSystolic blood pressureBody negative pressureNervous system activityAngiotensin-converting enzymeIR womenCardiovascular riskSerum aldosteroneBaroreflex controlDiastolic BPLetter to the Editor in response to: Microvascular endothelial function following the cessation of long‐term oral contraceptive pill use: A case report
Kirby N, Stachenfeld N, Alexander L. Letter to the Editor in response to: Microvascular endothelial function following the cessation of long‐term oral contraceptive pill use: A case report. Quarterly Journal Of Experimental Physiology And Cognate Medical Sciences 2023, 108: 1092-1094. PMID: 37098953, PMCID: PMC10836611, DOI: 10.1113/ep091174.Commentaries, Editorials and LettersEffects of androgen excess and body mass index on endothelial function in women with polycystic ovary syndrome
Berbrier D, Leone C, Adler T, Bender J, Taylor H, Stachenfeld N, Usselman C. Effects of androgen excess and body mass index on endothelial function in women with polycystic ovary syndrome. Journal Of Applied Physiology 2023, 134: 868-878. PMID: 36861670, DOI: 10.1152/japplphysiol.00583.2022.Peer-Reviewed Original ResearchConceptsPolycystic ovary syndromeEndothelial dysfunctionEndothelial functionOvary syndromeAndrogen excessLean womenObese womenEstradiol supplementationLow flow-mediated constrictionOW/OB groupOverweight/obese womenMarked endothelial dysfunctionSevere endothelial dysfunctionFree testosterone levelsBody mass indexFlow-mediated dilation (FMD) testFree testosteroneMass indexReactive hyperemiaOB groupTestosterone levelsHealth differEndothelial pathophysiologyDysfunctionAndrogens
2022
Correlations between salivary- and blood-derived gonadal hormone assessments and implications for inclusion of female participants in research studies
Huang T, Howse F, Stachenfeld N, Usselman C. Correlations between salivary- and blood-derived gonadal hormone assessments and implications for inclusion of female participants in research studies. AJP Heart And Circulatory Physiology 2022, 324: h33-h46. PMID: 36426884, DOI: 10.1152/ajpheart.00399.2022.Peer-Reviewed Original ResearchConceptsMenstrual cycleGonadal hormonesFemale participantsVenous blood samplesGonadal hormone fluctuationsPrimary gonadalVascular functionBlood concentrationsCardiovascular diseaseHormone assessmentSerial measurementsHormone fluctuationsHormone monitoringBlood samplesStudy designGold standard measurementHuman physiology researchHormoneInclusion of femalesSalivaryResearch studiesCardiovascular researchersParticipantsFemalesSeven days of statin treatment improves nitric-oxide mediated endothelial-dependent cutaneous microvascular function in women with endometriosis
Dillon GA, Stanhewicz AE, Serviente C, Flores VA, Stachenfeld N, Alexander LM. Seven days of statin treatment improves nitric-oxide mediated endothelial-dependent cutaneous microvascular function in women with endometriosis. Microvascular Research 2022, 144: 104421. PMID: 35970408, PMCID: PMC9527706, DOI: 10.1016/j.mvr.2022.104421.Peer-Reviewed Original ResearchConceptsEndothelium-dependent vasodilationLaser Doppler fluxEndothelial functionCardiovascular diseaseOral atorvastatinExerts potent anti-inflammatory effectsEndothelium-dependent agonist acetylcholineShort-term statin administrationPotent anti-inflammatory effectsIntradermal microdialysis perfusionNO-dependent dilationImpaired endothelial functionMicrovascular endothelial functionCutaneous microvascular functionCutaneous vascular conductanceAnti-inflammatory effectsNO-dependent vasodilationNO synthase inhibitorDose-response curveStatin therapyAdjunctive therapyAtorvastatin treatmentCVD riskEndothelial dysfunctionStatin administrationAre there sex differences in risk for exertional heat stroke? A translational approach
Giersch GEW, Garcia CK, Stachenfeld N, Charkoudian N. Are there sex differences in risk for exertional heat stroke? A translational approach. Quarterly Journal Of Experimental Physiology And Cognate Medical Sciences 2022, 107: 1136-1143. PMID: 35598159, DOI: 10.1113/ep090402.Peer-Reviewed Original ResearchConceptsExertional heat strokeHeat strokeRisk factorsTranslational approachCentral nervous system dysfunctionNervous system dysfunctionFemale sex hormonesSex differencesHuman researchOrgan damageFatal conditionSystem dysfunctionSex hormonesPhysical activityStrokeTranslational modelOccupational workersRiskMilitary personnelAnimalsTreatmentHuman modelReviewFuture investigationsPhysiological differencesSubclinical cardiovascular disease and polycystic ovary syndrome
Gomez JMD, VanHise K, Stachenfeld N, Chan JL, Merz NB, Shufelt C. Subclinical cardiovascular disease and polycystic ovary syndrome. Fertility And Sterility 2022, 117: 912-923. PMID: 35512975, PMCID: PMC10322116, DOI: 10.1016/j.fertnstert.2022.02.028.Peer-Reviewed Original ResearchConceptsPolycystic ovary syndromeSubclinical cardiovascular diseaseCardiovascular diseaseOvary syndromeCoronary artery calcium scoreCarotid intima-media thicknessOvert cardiovascular diseaseArtery calcium scoreFlow-mediated dilationIntima-media thicknessCalcium scoreDiabetes mellitusMetabolic syndromeArterial stiffnessIrregular mensesInsulin resistanceEpicardial fatMetabolic disordersVascular endotheliumSyndromeHallmark featureDiseaseWomenHyperandrogenismDyslipidemiaPrecision medicine requires understanding how both sex and gender influence health
Stachenfeld NS, Mazure CM. Precision medicine requires understanding how both sex and gender influence health. Cell 2022, 185: 1619-1622. PMID: 35561661, DOI: 10.1016/j.cell.2022.04.012.Peer-Reviewed Original ResearchEndometriosis promotes atherosclerosis in a murine model
Mamillapalli R, Toffoloni N, Habata S, Qunhua H, Atwani R, Stachenfeld N, Taylor HS. Endometriosis promotes atherosclerosis in a murine model. American Journal Of Obstetrics And Gynecology 2022, 227: 248.e1-248.e8. PMID: 35351413, PMCID: PMC9308711, DOI: 10.1016/j.ajog.2022.03.040.Peer-Reviewed Original ResearchConceptsEffect of endometriosisOil Red O stainingVascular endothelial growth factorMurine modelCardiovascular diseaseRed O stainingEndothelial growth factorSham miceEndometriotic lesionsInflammatory cytokinesInterleukin-6Luminal areaInterferon gammaO stainingGrowth factorAortic luminal areasInflammatory-related cytokinesRoot wall thicknessUnderwent sham surgeryTreatment of endometriosisInflammation-related cytokinesDevelopment of atherosclerosisApolipoprotein E (ApoE<sup>-/-</sup>) miceLong-term riskWeeks of inductionConsequences of hyperandrogenemia during pregnancy in female offspring: attenuated response to angiotensin II
Shawky NM, Dalmasso C, Ojeda NB, Zuchowski Y, Stachenfeld N, Alexander BT, Reckelhoff JF. Consequences of hyperandrogenemia during pregnancy in female offspring: attenuated response to angiotensin II. Journal Of Hypertension 2022, 40: 712-722. PMID: 34980865, PMCID: PMC8897268, DOI: 10.1097/hjh.0000000000003067.Peer-Reviewed Original ResearchConceptsPolycystic ovary syndromeAttenuated pressor responsesPressor responseBlood pressureAng IIHyperandrogenemic femalesAngiotensin IIFemale offspringModel of PCOSNitrate/nitrite excretionSimilar baseline blood pressureIntrarenal Ang IIBaseline blood pressureElevated blood pressureExaggerated pressor responseHigh-salt dietLow birth weightFemale control offspringDihydrotestosterone pelletsMaternal hyperandrogenemiaMore proteinuriaPCOS womenOvary syndromeRenal injurySalt diet
2020
Last Word on Point:Counterpoint: Investigators should/should not control for menstrual cycle phase when performing studies of vascular control that include women
Wenner MM, Stachenfeld NS. Last Word on Point:Counterpoint: Investigators should/should not control for menstrual cycle phase when performing studies of vascular control that include women. Journal Of Applied Physiology 2020, 129: 1136-1137. PMID: 33197373, DOI: 10.1152/japplphysiol.00860.2020.Commentaries, Editorials and LettersPathophysiological effects of androgens on the female vascular system
Stone T, Stachenfeld NS. Pathophysiological effects of androgens on the female vascular system. Biology Of Sex Differences 2020, 11: 45. PMID: 32727622, PMCID: PMC7391603, DOI: 10.1186/s13293-020-00323-6.ChaptersConceptsFemale cardiovascular systemPolycystic ovary syndromeFemale vasculatureHormone therapyOvary syndromePathophysiological effectsCardiovascular systemTransgender menPrimary medical interventionSignificant medical risksHigh blood pressureCardiovascular disease statesEffects of testosteroneEndogenous androgen concentrationsSex differencesPotential causative linkChronic hyperandrogenismTestosterone dosesEndothelial dysfunctionBlood pressurePatient groupVascular functionCardiovascular regulationSex hormonesAndrogen receptorPoint: Investigators should control for menstrual cycle phase when performing studies of vascular control that include women
Wenner MM, Stachenfeld NS. Point: Investigators should control for menstrual cycle phase when performing studies of vascular control that include women. Journal Of Applied Physiology 2020, 129: 1114-1116. PMID: 32702264, DOI: 10.1152/japplphysiol.00443.2020.Commentaries, Editorials and LettersIntroducing a special series, sex as a variable in human research: A systems approach
Stachenfeld N. Introducing a special series, sex as a variable in human research: A systems approach. The FASEB Journal 2020, 34: 8776-8777. PMID: 32946158, DOI: 10.1096/fj.202001425.Commentaries, Editorials and LettersMenstrual cycle and thermoregulation during exercise in the heat: A systematic review and meta-analysis
Giersch GEW, Morrissey MC, Katch RK, Colburn AT, Sims ST, Stachenfeld NS, Casa DJ. Menstrual cycle and thermoregulation during exercise in the heat: A systematic review and meta-analysis. Journal Of Science And Medicine In Sport 2020, 23: 1134-1140. PMID: 32499153, DOI: 10.1016/j.jsams.2020.05.014.Peer-Reviewed Original ResearchConceptsSystematic reviewHeart rateSweat rateSkin temperatureMenstrual cycle phaseMenstrual cycle variationsAerobic exerciseFollicular phaseMenstrual cycleLuteal phaseInclusion criteriaMenstrual phaseLimited available dataMean skin temperatureEffect size estimatesSignificant differencesBody temperatureEffect sizeExerciseWomenCycle phaseInternal body temperatureReviewTotal numberA recent history of preeclampsia is associated with elevated central pulse wave velocity and muscle sympathetic outflow
Usselman CW, Adler TE, Coovadia Y, Leone C, Paidas MJ, Stachenfeld NS. A recent history of preeclampsia is associated with elevated central pulse wave velocity and muscle sympathetic outflow. AJP Heart And Circulatory Physiology 2020, 318: h581-h589. PMID: 32004082, DOI: 10.1152/ajpheart.00578.2019.Peer-Reviewed Original ResearchConceptsMuscle sympathetic nerve activitySympathetic nerve activityArterial stiffnessBaroreflex sensitivityPreeclamptic womenUncomplicated pregnanciesNerve activityBlood pressureCardiovascular diseaseCentral pulse wave velocityCardiovagal baroreflex sensitivityElevated arterial stiffnessMuscle sympathetic outflowElevated cardiovascular riskHistory of preeclampsiaNormal blood pressurePeripheral arterial stiffnessSympathetic baroreflex sensitivitySympathetic nervous systemPulse wave velocityHigher MSNACardiovascular dysregulationSympathetic outflowCardiovascular riskNormal pregnancy