2017
Dietary protein and bone health: a systematic review and meta-analysis from the National Osteoporosis Foundation 1 , 2
Shams-White MM, Chung M, Du M, Fu Z, Insogna KL, Karlsen MC, LeBoff MS, Shapses SA, Sackey J, Wallace TC, Weaver CM. Dietary protein and bone health: a systematic review and meta-analysis from the National Osteoporosis Foundation 1 , 2. American Journal Of Clinical Nutrition 2017, 105: 1528-1543. PMID: 28404575, DOI: 10.3945/ajcn.116.145110.Peer-Reviewed Original ResearchConceptsBone mineral densityHigh protein intakeProspective cohort studyProtein intakeSystematic reviewCohort studyBone healthTotal hipFemoral neckModerate evidenceLumbar spine bone mineral densityTotal body bone mineral densityTH bone mineral densityLS bone mineral densityBody bone mineral densitySpine bone mineral densityFN bone mineral densityBone health measuresBone health outcomesDietary proteinLow protein intakeFull-text screeningStrength of evidenceOverall RoBNonpharmacologic approaches
2015
Dietary Protein and Vitamin D Intake and Risk of Falls: A Secondary Analysis of Postmenopausal Women from the Study of Osteoporotic Fractures
Larocque SC, Kerstetter JE, Cauley JA, Insogna KL, Ensrud K, Lui LY, Allore HG. Dietary Protein and Vitamin D Intake and Risk of Falls: A Secondary Analysis of Postmenopausal Women from the Study of Osteoporotic Fractures. Journal Of Nutrition In Gerontology And Geriatrics 2015, 34: 305-318. PMID: 26267443, PMCID: PMC4547350, DOI: 10.1080/21551197.2015.1054574.Peer-Reviewed Original ResearchConceptsVitamin D intakeVitamin DD intakePostmenopausal womenOsteoporotic fracturesDietary proteinOdds ratioOlder adultsFood frequency questionnaireRisk of fallsOccurrence of fallsIncident fallsFrequency questionnaireProspective cohortHip fractureMusculoskeletal healthInadequate intakeAdequate intakeOlder womenProtein intakeMuscle massSecondary analysisLogistic regressionIntakeWomenThe effects of dietary protein and amino acids on skeletal metabolism
Bihuniak JD, Insogna KL. The effects of dietary protein and amino acids on skeletal metabolism. Molecular And Cellular Endocrinology 2015, 410: 78-86. PMID: 25843057, PMCID: PMC5852680, DOI: 10.1016/j.mce.2015.03.024.BooksConceptsDietary proteinDietary protein intakeOptimal skeletal growthRecent data bearingSkeletal healthCalcium economyMineral metabolismSkeletal metabolismProtein intakeDietary allowanceSkeletal homeostasisNutrient intakeSkeletal massIntakeSkeletal growthSite-specific effectsMolecular mechanismsMetabolismVariable levelsLife continuumProtein
2014
Supplementing a Low-Protein Diet with Dibasic Amino Acids Increases Urinary Calcium Excretion in Young Women 1,2
Bihuniak JD, Sullivan RR, Simpson CA, Caseria DM, Huedo-Medina TB, O’Brien K, Kerstetter JE, Insogna KL. Supplementing a Low-Protein Diet with Dibasic Amino Acids Increases Urinary Calcium Excretion in Young Women 1,2. Journal Of Nutrition 2014, 144: 282-288. PMID: 24431325, PMCID: PMC3927545, DOI: 10.3945/jn.113.185009.Peer-Reviewed Original ResearchConceptsIntestinal calcium absorptionLow-protein dietCalcium absorptionDual-stable calcium isotopesYoung womenUrinary calcium excretionDietary proteinControl diet periodAmino acid L-arginineHigh-protein dietAmino acid L-tryptophanCalcium excretionDiet periodClinical trialsSupplementation periodL-arginineDay 5Control dietRandom orderPhysiologic rangeDietTrialsWomenPossible benefitsL-tryptophan
2013
The effect of selective amino acid supplementation on calcium absorption during a low protein diet
Bihuniak J, Sullivan R, Simpson C, Caseria D, O'Brien K, Kerstetter J, Insogna K. The effect of selective amino acid supplementation on calcium absorption during a low protein diet. The FASEB Journal 2013, 27: lb283-lb283. DOI: 10.1096/fasebj.27.1_supplement.lb283.Peer-Reviewed Original ResearchLow-protein dietIntestinal Ca absorptionCa absorptionProtein dietProtein intakeDietary protein intakeHigh-protein dietAmino acid supplementationCalcium absorptionAcid supplementationDay 5Dibasic amino acidsAA supplementationYoung womenControl dietRandom orderPhysiologic rangeDietary proteinIntakeDietSupplementationAmino acidsAromatic amino acidsCa economyLys supplementationIncreasing dietary protein selectively upregulates the DMT1 isoform that contains an iron responsive element
Thomas C, Yao G, O'Brien K, Kerstetter J, Insogna K. Increasing dietary protein selectively upregulates the DMT1 isoform that contains an iron responsive element. The FASEB Journal 2013, 27: lb284-lb284. DOI: 10.1096/fasebj.27.1_supplement.lb284.Peer-Reviewed Original ResearchIntestinal iron absorptionIron absorptionDietary proteinProtein dietMRNA expressionWhole-body iron homeostasisDuodenum of ratsDMT1 mRNA expressionBody iron homeostasisGenetic absenceExperimental animalsLevel of expressionResponsive elementBrush border membraneRatsIron-responsive elementRecent dataDietCritical regulatorIron homeostasisBorder membraneSignificant changesExpressionIron importerAnimalsIncreasing dietary protein acutely augments intestinal iron transporter expression and significantly increases iron absorption in rats
Thomas CE, Gaffney‐Stomberg E, Sun B, O'Brien KO, Kerstetter JE, Insogna KL. Increasing dietary protein acutely augments intestinal iron transporter expression and significantly increases iron absorption in rats. The FASEB Journal 2013, 27: 2476-2483. PMID: 23447582, DOI: 10.1096/fj.12-221945.Peer-Reviewed Original ResearchConceptsIntestinal Fe absorptionDietary proteinFe absorptionIron transporter expressionTranscript expressionDuodenal mucosaAcute effectsSprague-DawleyStable iron isotopesIron absorptionShort-term effectsIsocaloric dietsIron deficiencyTransporter expressionLittle dataTransferrin receptorExpression of genesSeparate studiesGroupCurrent studyBalance studiesExpressionWhole genome profilingDawleyMucosaClaudins, dietary milk proteins, and intestinal barrier regulation
Kotler BM, Kerstetter JE, Insogna KL. Claudins, dietary milk proteins, and intestinal barrier regulation. Nutrition Reviews 2013, 71: 60-65. PMID: 23282252, DOI: 10.1111/j.1753-4887.2012.00549.x.BooksConceptsDietary milk proteinsIntestinal barrier regulationClaudin expressionIntestinal barrier dysfunctionIntestinal barrier functionLimited clinical studiesClaudin-4 expressionGrowth factor βMechanism of actionBarrier dysfunctionIntestinal barrierTherapeutic roleClinical studiesBarrier functionDietary proteinFactor βTight junctionsBarrier regulationClaudin proteinsRecent studiesProtein componentsExpressionMilk protein componentsMilk proteinsProtein
2011
Dietary Protein’s Impact on Skeletal Health
Surdykowski A, Kenney A, Insogna K, Kerstetter J. Dietary Protein’s Impact on Skeletal Health. 2011, 237-246. DOI: 10.1201/b11228-18.Peer-Reviewed Original ResearchInsulin-like growth factor-1Skeletal healthLoss of calciumUrinary calcium excretionIntestinal calcium absorptionNegative calcium balanceGrowth factor-1Skeletal storesCalcium excretionVitamin DDietary calciumMuscle strengthCalcium absorptionCalcium metabolismSystemic acidosisBone balanceCalcium balanceHigh intakeDietary proteinFactor 1HealthIntense investigationCalciumProtein impactMetabolismDietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression
Cucchi C, Gaffney‐Stomberg E, Sun B, O'Brien K, Kerstetter J, Insogna K. Dietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression. The FASEB Journal 2011, 25: lb193-lb193. DOI: 10.1096/fasebj.25.1_supplement.lb193.Peer-Reviewed Original ResearchProtein dietDietary proteinProtein expressionCaco-2 BBe cellsIntestinal Fe absorptionFe absorption studiesIntestinal iron absorptionTranscript expressionAmino acid-free mediumDMT1 protein levelsAmino acid treatmentDMT1 protein expressionProtein doseIron absorptionBBE cellsIron deficiencyAcid-free mediumRatsProtein levelsDietDMT1 transcriptsAmino acidsFe absorptionWestern analysisKey regulatorDietary protein increases intestinal calcium absorption in part by increasing tight junction expression of Claudin‐2
Gaffney‐Stomberg E, Cucchi C, Sun B, Bataille A, Renfro L, Kerstetter J, Insogna K. Dietary protein increases intestinal calcium absorption in part by increasing tight junction expression of Claudin‐2. The FASEB Journal 2011, 25: 983.30-983.30. DOI: 10.1096/fasebj.25.1_supplement.983.30.Peer-Reviewed Original ResearchHigh-protein dietCaco-2 BBe cellsClaudin-2 expressionCa absorptionCalcium absorptionProtein dietClaudin-2BBE cellsFemale Sprague-Dawley ratsIntestinal calcium absorptionDietary proteinSprague-Dawley ratsTight junction expressionCa transportTight junction proteinsTranscellular Ca transportDietary protein effectsCa-transporting proteinsDuodenal mucosaAcute effectsDay 7MRNA expressionJunction expressionJunction proteinsAmino acidsDietary protein and skeletal health: a review of recent human research
Kerstetter JE, Kenny AM, Insogna KL. Dietary protein and skeletal health: a review of recent human research. Current Opinion In Lipidology 2011, 22: 16-20. PMID: 21102327, PMCID: PMC4659357, DOI: 10.1097/mol.0b013e3283419441.BooksConceptsSkeletal healthBone healthDietary proteinBone resorptionCalcium retentionInsulin-like growth factor-1Recent human researchOsteoclastic bone resorptionBone mineral densityHigh protein intakeLean body massGrowth factor-1High-protein dietMeta-analysis studyVitamin DDietary calciumCalcium absorptionCalcium metabolismBone metabolismClinical trialsMineral densityBone balanceBone massMetabolic acid loadSystemic pH
2010
Optimizing bone health in older adults: the importance of dietary protein
Surdykowski AK, Kenny AM, Insogna KL, Kerstetter JE. Optimizing bone health in older adults: the importance of dietary protein. Aging Health 2010, 6: 345-357. PMID: 20657805, PMCID: PMC2907525, DOI: 10.2217/ahe.10.16.BooksBone healthBone lossDietary proteinAge-related bone lossDietary protein recommendationsSkeletal healthVitamin DDietary calciumMuscle strengthCalcium absorptionCalcium metabolismBone balanceIGF-1Protein intakeProtein recommendationsOlder adultsHealthModerate increasePivotal roleOsteoporosisProtein contributesProteinIntakeA High Protein Diet Induces Intestinal Iron Transporter Expression and Improves Iron Absorption in Rats
Cucchi C, Gaffney‐Stomberg E, Sun B, Kerstetter J, Insogna K. A High Protein Diet Induces Intestinal Iron Transporter Expression and Improves Iron Absorption in Rats. The FASEB Journal 2010, 24: 229.1-229.1. DOI: 10.1096/fasebj.24.1_supplement.229.1.Peer-Reviewed Original ResearchDietary proteinIron absorptionTransporter expressionHigh casein dietExpression of DMT1Iron transporter expressionFe absorptionVs. 5Kruskal-Wallis testMicroarray screenCasein dietMineral homeostasisLevel of expressionProtein dietRatsLittle dataDMT1 expressionQ-PCRDietBalance studiesExpressionHomeostasisGroupDMT1CYBRD1The Effect of Dietary Protein on Intestinal Calcium Absorption in Rats
Gaffney-Stomberg E, Sun BH, Cucchi CE, Simpson CA, Gundberg C, Kerstetter JE, Insogna KL. The Effect of Dietary Protein on Intestinal Calcium Absorption in Rats. Endocrinology 2010, 151: 1071-1078. PMID: 20147526, PMCID: PMC2840679, DOI: 10.1210/en.2009-0744.Peer-Reviewed Original ResearchConceptsIntestinal Ca absorptionCa absorptionHigh-protein dietUrinary calciumBrush border membrane vesiclesProtein intakeFemale Sprague-Dawley ratsProtein dietProtein intake resultsIntestinal calcium absorptionDietary protein intakeHigh protein intakeSprague-Dawley ratsLow-protein groupHigh protein groupDietary protein effectsCalcium absorptionAcute increaseDawley ratsDuodenal mucosaIntake resultsRatsCa balanceTranscellular CaDietary protein
2009
Increasing Dietary Protein Requirements in Elderly People for Optimal Muscle and Bone Health
Gaffney‐Stomberg E, Insogna KL, Rodriguez NR, Kerstetter JE. Increasing Dietary Protein Requirements in Elderly People for Optimal Muscle and Bone Health. Journal Of The American Geriatrics Society 2009, 57: 1073-1079. PMID: 19460090, DOI: 10.1111/j.1532-5415.2009.02285.x.BooksConceptsRecommended Dietary AllowanceCurrent Recommended Dietary AllowanceDietary proteinImportant public health goalOlder individualsInsulin-like growth factorElderly peopleNormal calcium metabolismLoss of boneMuscle protein synthesisShort-term proteinMaintenance of musclePublic health goalsRenal functionBone healthSignificant morbidityCalcium absorptionCalcium metabolismSupplement trialAnabolic effectsClinical studiesProtein adequacyAnabolic responseProtein intakeMuscle massA Rodent Model to Evaluate the Effect of Dietary Protein on Intestinal Calcium Absorption
Gaffney‐Stomberg E, Cucchi C, Sun B, Simpson C, Kerstetter J, Insogna K. A Rodent Model to Evaluate the Effect of Dietary Protein on Intestinal Calcium Absorption. The FASEB Journal 2009, 23: 726.1-726.1. DOI: 10.1096/fasebj.23.1_supplement.726.1.Peer-Reviewed Original ResearchHigh-protein dietLow-protein groupCa absorptionProtein dietBone turnoverBrush border membrane vesiclesIntestinal Ca absorptionIntestinal calcium absorptionDietary proteinVitamin D metabolitesLow protein intakeCa transportLow-protein dietSerum PTHSerum osteocalcinHr urineCalcium absorptionD metabolitesSerum CaDuodenal mucosaUrine CaRodent modelsAnimal modelsProtein intakeCa metabolism
2007
Dietary protein increases intestinal calcium absorption and improves bone balance: An hypothesis
Kerstetter J, Gaffney E, O'Brien K, Caseria D, Insogna K. Dietary protein increases intestinal calcium absorption and improves bone balance: An hypothesis. International Congress Series 2007, 1297: 204-216. DOI: 10.1016/j.ics.2006.08.027.Peer-Reviewed Original ResearchBone mineral densityIntestinal calcium absorptionHigh-protein dietUrinary calciumBone lossCalcium absorptionProtein dietAccelerated bone lossMost cross-sectional studiesDietary proteinCross-sectional studyLow-protein dietBone turnoverBone resorptionMineral densityBone balanceCalcium studiesCalcium retentionSalutary effectsBone originPrincipal outcomeDietSignificant reductionCalciumResorption
2006
Meat and Soy Protein Affect Calcium Homeostasis in Healthy Women 1 , 2
Kerstetter JE, Wall DE, O’Brien K, Caseria DM, Insogna KL. Meat and Soy Protein Affect Calcium Homeostasis in Healthy Women 1 , 2. Journal Of Nutrition 2006, 136: 1890-1895. PMID: 16772455, DOI: 10.1093/jn/136.7.1890.Peer-Reviewed Original ResearchConceptsIntestinal calcium absorptionUrinary calciumCalcium absorptionLow-protein dietHealthy womenCalcium homeostasisHigher protein interventionNet acid excretionSubset of subjectsHigh-protein dietSoy interventionParathyroid hormoneExperimental periodSerum concentrationsAcid excretionSoy dietAcute declineWomen 1Calcium bioavailabilityRandom orderDietary proteinMeat dietDietSoy proteinIntervention
2004
The Impact of Dietary Protein on Calcium Absorption and Kinetic Measures of Bone Turnover in Women
Kerstetter JE, O’Brien K, Caseria DM, Wall DE, Insogna KL. The Impact of Dietary Protein on Calcium Absorption and Kinetic Measures of Bone Turnover in Women. The Journal Of Clinical Endocrinology & Metabolism 2004, 90: 26-31. PMID: 15546911, DOI: 10.1210/jc.2004-0179.Peer-Reviewed Original ResearchConceptsHigh-protein dietUrinary calciumBone turnoverCalcium absorptionDual-stable calcium isotopesAdditional urinary calciumIntestinal calcium absorptionHealthy womenStable calcium isotopesBone balanceNonsignificant trendSkeletal resorptionCalcium kineticsAcid loadBone originProtein-induced effectsLevels of proteinRandom orderDietary proteinDietHypercalciuriaWomenExperimental dietsSignificant reductionCalcium