2004
The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells
Cook HL, Mischo HE, Steitz JA. The Herpesvirus saimiri Small Nuclear RNAs Recruit AU-Rich Element-Binding Proteins but Do Not Alter Host AU-Rich Element-Containing mRNA Levels in Virally Transformed T Cells. Molecular And Cellular Biology 2004, 24: 4522-4533. PMID: 15121869, PMCID: PMC400482, DOI: 10.1128/mcb.24.10.4522-4533.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceBase CompositionBase SequenceCallithrixCell LineCell Transformation, ViralELAV ProteinsELAV-Like Protein 1Herpesvirus 2, SaimiriineHeterogeneous-Nuclear Ribonucleoprotein DIn Vitro TechniquesMolecular Sequence DataMutationNucleic Acid ConformationProtein BindingRNA-Binding ProteinsRNA, MessengerRNA, Small NuclearRNA, ViralT-LymphocytesConceptsAU-rich elementsSmall nuclear RNAHSURs 1Herpesvirus saimiriNuclear RNAMRNA decay pathwayMarmoset T cellsHSUR 1HnRNP DPosttranscriptional regulationHost mRNAsHost proteinsMicroarray analysisUnknown functionProtein tristetraprolinVivo interactionDecay pathwaysHSURsMRNARNAMRNA levelsT cellsProteinCellsPathway
1997
Identification of HuR as a protein implicated in AUUUA‐mediated mRNA decay
Myer V, Fan X, Steitz J. Identification of HuR as a protein implicated in AUUUA‐mediated mRNA decay. The EMBO Journal 1997, 16: 2130-2139. PMID: 9155038, PMCID: PMC1169815, DOI: 10.1093/emboj/16.8.2130.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsAntigens, SurfaceBase CompositionCell ExtractsCross-Linking ReagentsELAV ProteinsELAV-Like Protein 1Gene Expression RegulationHeLa CellsHumansMiceMolecular Sequence DataMolecular WeightRegulatory Sequences, Nucleic AcidRNA-Binding ProteinsRNA, MessengerUltraviolet RaysConceptsAU-rich elementsMRNA decayUntranslated regionRNA-binding specificityARE-binding proteinsHeLa nuclear extractsGene familyMRNA degradationNuclear extractsEssential signalMessenger RNAProteinSequence's abilityHuRAUUUARapid degradationCritical roleHuR.RNAMachineryMRNADegradationRegulationSubsequent analysisExpression
1996
Highly Diverged U4 and U6 Small Nuclear RNAs Required for Splicing Rare AT-AC Introns
Tarn W, Steitz J. Highly Diverged U4 and U6 Small Nuclear RNAs Required for Splicing Rare AT-AC Introns. Science 1996, 273: 1824-1832. PMID: 8791582, DOI: 10.1126/science.273.5283.1824.Peer-Reviewed Original ResearchA Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro
Tarn W, Steitz J. A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro. Cell 1996, 84: 801-811. PMID: 8625417, DOI: 10.1016/s0092-8674(00)81057-0.Peer-Reviewed Original ResearchMeSH KeywordsBase CompositionBase SequenceBlotting, NorthernCell NucleusHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPlasmidsPolymerase Chain ReactionRibonuclease HRibonucleoprotein, U5 Small NuclearRibonucleoproteins, Small NuclearRNA PrecursorsRNA SplicingConceptsU5 small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinU12 small nuclear ribonucleoproteinsMinor class intronsProtein coding genesPre-mRNA substrateNative gel electrophoresisCoding genesBranch site sequenceSplicing complexesNuclear ribonucleoproteinPre-mRNAP120 geneLariat intermediateSite sequenceIntronsHeLa cellsEssential roleSplicingGel electrophoresisBranch siteGenesU12Minor classU11
1995
ENHANCED PERSPECTIVE: Small RNA Chaperones for Ribosome Biogenesis
Steitz J, Tycowski K. ENHANCED PERSPECTIVE: Small RNA Chaperones for Ribosome Biogenesis. Science 1995, 270: 1626-1626. PMID: 7502072, DOI: 10.1126/science.270.5242.1626.Peer-Reviewed Original Research
1991
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
Bond UM, Yario TA, Steitz JA. Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro. Genes & Development 1991, 5: 1709-1722. PMID: 1885007, DOI: 10.1101/gad.5.9.1709.Peer-Reviewed Original ResearchConceptsHistone downstream elementHistone pre-mRNAMammalian histone pre-mRNAsPre-mRNAHeLa cellsBase pair regionMammalian histonesU7 geneSm snRNPsU7 snRNPGenetic evidenceU7 snRNAUnexpected toleranceU7 RNANuclear extractsDownstream elementsSuppressor geneCompensatory changesGenesBlock substitutionsRNAVivoSnRNPsSnRNPCellsStructural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function.
Wassarman DA, Steitz JA. Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function. Molecular And Cellular Biology 1991, 11: 3432-3445. PMID: 1646389, PMCID: PMC361072, DOI: 10.1128/mcb.11.7.3432.Peer-Reviewed Original Research
1988
An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the α-sarcin site
Parker K, Bruzik J, Steitz J. An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the α-sarcin site. Nucleic Acids Research 1988, 16: 10493-10509. PMID: 2974535, PMCID: PMC338920, DOI: 10.1093/nar/16.22.10493.Peer-Reviewed Original ResearchAntibodiesBase CompositionBase SequenceEndoribonucleasesFungal ProteinsHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationPlasmidsProtein Synthesis InhibitorsRibonuclease T1RibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA, RibosomalRNA, Ribosomal, 28STranscription, GeneticFour novel U RNAs are encoded by a herpesvirus
Lee S, Murthy S, Trimble J, Desrosiers R, Steitz J. Four novel U RNAs are encoded by a herpesvirus. Cell 1988, 54: 599-607. PMID: 2842058, DOI: 10.1016/s0092-8674(88)80004-7.Peer-Reviewed Original ResearchConceptsHSUR 1U RNAU RNA genesTrimethylguanosine cap structureSmall nuclear ribonucleoproteinEnd formation signalsAAUAAA polyadenylation signalBase pairing interactionsRNA genesMarmoset T lymphocytesPolyadenylation signalNuclear ribonucleoproteinCap structureEnd sequencesPerfect complementarityViral genesSnRNP particlesLow abundanceHerpesvirus saimiriRNASm determinantsGenesFormation signalEnd regionSequence
1987
Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
Parker KA, Steitz JA. Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA. Molecular And Cellular Biology 1987, 7: 2899-2913. PMID: 2959855, PMCID: PMC367909, DOI: 10.1128/mcb.7.8.2899.Peer-Reviewed Original ResearchConceptsProtein-RNA interactionsSecondary structureSubsequent reverse transcriptionRNA secondary structureHuman U3Phosphorylated proteinsRibonucleoprotein particleProcessing eventsAlternative functionsBase pairsNucleotides -159Specific nucleasesHeLa cellsProtein constituentsRNase ARNPProteinNonphosphorylated proteinsRRNAReverse transcriptionSequenceKilodaltonsNucleotides 65Specific reagentsTranscription
1984
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle
Hashimoto C, Steitz J. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle. Nucleic Acids Research 1984, 12: 3283-3293. PMID: 6201826, PMCID: PMC318745, DOI: 10.1093/nar/12.7.3283.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU6 RNARNA complexIntact small nuclear ribonucleoproteinRNA/RNA complexesHuman small nuclear ribonucleoproteinsSmall nuclear ribonucleoprotein particleRNA-RNA interactionsNuclear ribonucleoprotein particleSequence complementarityMammalian cellsNuclear ribonucleoproteinRibonucleoprotein particleSnRNP particlesRNABiological implicationsPolyacrylamide gelsProteinase KU4ComplexesSodium dodecyl sulfateRibonucleoproteinDodecyl sulfateU5U1
1979
The 3′ terminus of 16S rRNA: secondary structure and interaction with ribosomal protein S1
Yuan R, Steitz J, Moore P, Crothers D. The 3′ terminus of 16S rRNA: secondary structure and interaction with ribosomal protein S1. Nucleic Acids Research 1979, 7: 2399-2418. PMID: 392471, PMCID: PMC342392, DOI: 10.1093/nar/7.8.2399.Peer-Reviewed Original Research