2008
Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3′‐end maturation
Kolev NG, Yario TA, Benson E, Steitz JA. Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3′‐end maturation. EMBO Reports 2008, 9: 1013-1018. PMID: 18688255, PMCID: PMC2572124, DOI: 10.1038/embor.2008.146.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceBase SequenceCell LineCleavage And Polyadenylation Specificity FactorConserved SequenceEndonucleasesEnzyme ActivationHeLa CellsHistonesHumansMolecular Sequence DataProtein Structure, TertiaryProtein SubunitsRNA 3' End ProcessingRNA PrecursorsRNA, MessengerConceptsPre-messenger RNAPolyadenylation specificity factorMammalian proteinsRNase ZConserved motifsHistone mRNASpecificity factorEndonucleolytic cleavageActive endonucleaseEndonuclease activityMBL familyComplex machineryMessenger RNAPoint mutationsCPSF73CPSF100Process of maturationMaturation processRNAProteinMotifMRNAMaturationEukaryotesCleavageMinor-class splicing occurs in the nucleus of the Xenopus oocyte
Friend K, Kolev NG, Shu MD, Steitz JA. Minor-class splicing occurs in the nucleus of the Xenopus oocyte. RNA 2008, 14: 1459-1462. PMID: 18567814, PMCID: PMC2491479, DOI: 10.1261/rna.1119708.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinMinor class intronsU12-type splicingXenopus oocytesU12-dependent intronsNuclear envelope breakdownCertain eukaryotesMinor spliceosomeVertebrate cellsSplicing substrateNuclear compartmentNuclear ribonucleoproteinRNA intronsAccurate splicingEnvelope breakdownSplicingIntronsCytoplasmOocytesEukaryotesSpliceosomeMeiosisRibonucleoproteinNucleusSmall fraction
1988
Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.
Montzka KA, Steitz JA. Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 8885-8889. PMID: 2973606, PMCID: PMC282611, DOI: 10.1073/pnas.85.23.8885.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU12 small nuclear ribonucleoproteinsSm small nuclear ribonucleoproteinsTrimethylguanosine cap structureTwo-dimensional gel fractionationLow-abundance RNAsLower eukaryotesSmall RNAsMRNA processingMammalian cellsMRNA splicingNuclear ribonucleoproteinAdditional speciesCap structureNuclease sensitivityNew RNASm classSm epitopesRNAU11Gel fractionationPotential roleU12EukaryotesSplicing